中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
3期
554-556
,共3页
粒细胞集落刺激因子%心功能%脑钠肽%重塑
粒細胞集落刺激因子%心功能%腦鈉肽%重塑
립세포집락자격인자%심공능%뇌납태%중소
Granulocyte colony stimulating factor%Cardiac function%Brain natriuretic peptide%Remodeling
目的 观察粒细胞集落刺激因子(G-CSF)对兔心肌梗死后心脏功能及结构重塑的影响.方法 38只新西兰大白兔随机分为两组,于左心耳与心尖连线1/2处结扎左前降支,制备心肌梗死模型.术后24h,实验组G-CSF 10 μg/(kg·),对照组生理盐水0.5ml/(kg·d),共5d均皮下注射.在术前、术后60d二维超声测量心功能,酶联免疫吸附试验(ELISA)检测血浆脑钠肽(BNP)水平.结果 梗后60d,实验组与对照组超声结果分别为左室射血分数(LVEF)[(73.6±3.7)％、(60.1±12.0)％]、短轴缩短率(FS)[(43.5±3.7)％、(33.1±9.3)％]、左室收缩末期内径(LVESD)[(7.9±0.3) mm、(10.1±1.6) mm]、左室舒张末期内径(LVEDD)[(14.1±0.8)mm、(15.1±1.0) mm]、左房内径(LAD)[(7.14±0.24) mm、(15.1±1.0) mm]、左室后壁厚度(LVPWT) [(2.98±0.25) mm、(3.37±0.44) mm、室间隔厚度(IVST)[(2.91±0.19) mm、(3.28 ±0.42) mm],两组各参数之间差异有统计学意义(P＜0.05).BNP梗后第10天,实验组(113.38±39.40) ng/L,对照组(325.35±96.14) ng/L(P ＜0.05)；梗后60d,实验组:(34.90±13.90) ng/L,对照组:(150.14±37.15) ng/L(P＜0.05).结论 G-CSF可改善急性心肌梗死后心功能,并维持至慢性期,同时能改善心肌结构重塑.
目的 觀察粒細胞集落刺激因子(G-CSF)對兔心肌梗死後心髒功能及結構重塑的影響.方法 38隻新西蘭大白兔隨機分為兩組,于左心耳與心尖連線1/2處結扎左前降支,製備心肌梗死模型.術後24h,實驗組G-CSF 10 μg/(kg·),對照組生理鹽水0.5ml/(kg·d),共5d均皮下註射.在術前、術後60d二維超聲測量心功能,酶聯免疫吸附試驗(ELISA)檢測血漿腦鈉肽(BNP)水平.結果 梗後60d,實驗組與對照組超聲結果分彆為左室射血分數(LVEF)[(73.6±3.7)％、(60.1±12.0)％]、短軸縮短率(FS)[(43.5±3.7)％、(33.1±9.3)％]、左室收縮末期內徑(LVESD)[(7.9±0.3) mm、(10.1±1.6) mm]、左室舒張末期內徑(LVEDD)[(14.1±0.8)mm、(15.1±1.0) mm]、左房內徑(LAD)[(7.14±0.24) mm、(15.1±1.0) mm]、左室後壁厚度(LVPWT) [(2.98±0.25) mm、(3.37±0.44) mm、室間隔厚度(IVST)[(2.91±0.19) mm、(3.28 ±0.42) mm],兩組各參數之間差異有統計學意義(P＜0.05).BNP梗後第10天,實驗組(113.38±39.40) ng/L,對照組(325.35±96.14) ng/L(P ＜0.05)；梗後60d,實驗組:(34.90±13.90) ng/L,對照組:(150.14±37.15) ng/L(P＜0.05).結論 G-CSF可改善急性心肌梗死後心功能,併維持至慢性期,同時能改善心肌結構重塑.
목적 관찰립세포집락자격인자(G-CSF)대토심기경사후심장공능급결구중소적영향.방법 38지신서란대백토수궤분위량조,우좌심이여심첨련선1/2처결찰좌전강지,제비심기경사모형.술후24h,실험조G-CSF 10 μg/(kg·),대조조생리염수0.5ml/(kg·d),공5d균피하주사.재술전、술후60d이유초성측량심공능,매련면역흡부시험(ELISA)검측혈장뇌납태(BNP)수평.결과 경후60d,실험조여대조조초성결과분별위좌실사혈분수(LVEF)[(73.6±3.7)％、(60.1±12.0)％]、단축축단솔(FS)[(43.5±3.7)％、(33.1±9.3)％]、좌실수축말기내경(LVESD)[(7.9±0.3) mm、(10.1±1.6) mm]、좌실서장말기내경(LVEDD)[(14.1±0.8)mm、(15.1±1.0) mm]、좌방내경(LAD)[(7.14±0.24) mm、(15.1±1.0) mm]、좌실후벽후도(LVPWT) [(2.98±0.25) mm、(3.37±0.44) mm、실간격후도(IVST)[(2.91±0.19) mm、(3.28 ±0.42) mm],량조각삼수지간차이유통계학의의(P＜0.05).BNP경후제10천,실험조(113.38±39.40) ng/L,대조조(325.35±96.14) ng/L(P ＜0.05)；경후60d,실험조:(34.90±13.90) ng/L,대조조:(150.14±37.15) ng/L(P＜0.05).결론 G-CSF가개선급성심기경사후심공능,병유지지만성기,동시능개선심기결구중소.
Objective To observe the effect of granulocyte colony stimulating factor (G-CSF) on cardiac structure and function post myocardial infarction.Methods Left ventricular ejection fraction (LVEF),fractional shortening (FS),left ventricular end systolic diameter (LVESD),left ventricular end diastolic diameter (LVEDD),left atrial diameter (LAD),left ventricular posterior wall thickness (LVPWT),interventricular septal thickness (IVST) were measured by echocardiography before and post myocardial infarction (MI).Brain natriuretic peptide (BNP) was measured by enzyme linked immunosorbent assay (ELISA)double-antibodyaphy sandwich methods.Results All the parameters have no difference between two group before MI,At the 60 days post MI,the parameters in treatment group and control group are as follows:(73.6±3.7)％ via (60.1 ±12.0)％ for LVEF,(43.5 ±3.7)％ via (33.1 ±9.3)％ for FS,(7.9 ±0.3)mm via (10.1 ±1.6) mm for LVESD,(14.1 ±0.8) mm via (15.1 ±1.0) mm for LVEDD,(7.14±0.24) mm via (8.90 ±0.91) mm for LAD,(2.98 ±0.25) mm via (3.37 ±0.44) mm for LVPWT,(2.91 ±0.19) mm via (3.28 ±0.42) mm for IVST (P ＜0.05).The results of BNP in treatment group and control group are as follows:Both increased to the top at two hours post MI (P ＞ 0.05),(113.38 ± 39.40) ng/L via (325.35 ± 96.14) ng/L at the 10days post MI (P ＜ 0.05) ; (34.90 ±13.90) ng/L via (150.14 ± 37.15) ng/L at the 60 days post MI (P ＜ 0.05).Conclusion G-CSF can improve cardiac function and reduce heart structure remodeling post MI.
