中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
3期
567-570
,共4页
朱智能%朱朝辉%庞自力%兰东阳%王海鹏
硃智能%硃朝輝%龐自力%蘭東暘%王海鵬
주지능%주조휘%방자력%란동양%왕해붕
上皮间质转化%膀胱移行细胞癌%核转录因子%叉头框蛋白Q1
上皮間質轉化%膀胱移行細胞癌%覈轉錄因子%扠頭框蛋白Q1
상피간질전화%방광이행세포암%핵전록인자%차두광단백Q1
Epithelial mesenchymal transition%Bladder transitional cell carcinoma%Transcription factor%Forkhead box protein Q1
目的 探讨膀胱移行细胞癌(BTCC)上皮间质转化(EMT)与核转录因子叉头框蛋白Q1(FOXQ1)的相关性,以及FOXQ1与膀胱癌临床病理特征之间的关系.方法 选用膀胱移行细胞癌手术切除标本65例,每例标本均选用癌组织中心、癌旁组织及其远端正常黏膜组织对照.采用免疫组织化学、逆转录-聚合酶链反应(RT-PCR)和Western blot技术检测各例标本中转化生长因子(TGF)-β1、核转录因子FOXQ1及上皮间质标志物E-钙黏蛋白(E-cadhefin)和波形蛋白(Vimentin)的表达,并分析与临床病理因素之间的关系.结果 (1) FOXQ1在BTCC组织中的表达高于正常黏膜组织(89.2％比13.8％,P＜0.05),其在肌层浸润性癌中的表达高于浅表性癌(97.3％比78.6％),在有淋巴结转移癌中的表达明显高于无淋巴结转移癌(100.0％比86.8％)；TGF-β1在BTCC组织中的表达高于正常黏膜(76.9％比26.2％,P＜0.05)；E-cadherin在BTCC组织中的表达低于正常黏膜(27.7％比89.2％,P＜0.05)；Vimentin在BTCC组织中的表达高于正常黏膜(83.1％比20.0％,P＜0.05).(2) FOXQ1与E-cadherin表达呈负相关,与TGF-β1、Vimentin表达呈正相关,是EMT的正向调控因子.结论 核转录因子FOXQ1与膀胱移行细胞癌分化和转移有关,可能通过调控EMT来促进膀胱移行细胞癌的侵袭转移.
目的 探討膀胱移行細胞癌(BTCC)上皮間質轉化(EMT)與覈轉錄因子扠頭框蛋白Q1(FOXQ1)的相關性,以及FOXQ1與膀胱癌臨床病理特徵之間的關繫.方法 選用膀胱移行細胞癌手術切除標本65例,每例標本均選用癌組織中心、癌徬組織及其遠耑正常黏膜組織對照.採用免疫組織化學、逆轉錄-聚閤酶鏈反應(RT-PCR)和Western blot技術檢測各例標本中轉化生長因子(TGF)-β1、覈轉錄因子FOXQ1及上皮間質標誌物E-鈣黏蛋白(E-cadhefin)和波形蛋白(Vimentin)的錶達,併分析與臨床病理因素之間的關繫.結果 (1) FOXQ1在BTCC組織中的錶達高于正常黏膜組織(89.2％比13.8％,P＜0.05),其在肌層浸潤性癌中的錶達高于淺錶性癌(97.3％比78.6％),在有淋巴結轉移癌中的錶達明顯高于無淋巴結轉移癌(100.0％比86.8％)；TGF-β1在BTCC組織中的錶達高于正常黏膜(76.9％比26.2％,P＜0.05)；E-cadherin在BTCC組織中的錶達低于正常黏膜(27.7％比89.2％,P＜0.05)；Vimentin在BTCC組織中的錶達高于正常黏膜(83.1％比20.0％,P＜0.05).(2) FOXQ1與E-cadherin錶達呈負相關,與TGF-β1、Vimentin錶達呈正相關,是EMT的正嚮調控因子.結論 覈轉錄因子FOXQ1與膀胱移行細胞癌分化和轉移有關,可能通過調控EMT來促進膀胱移行細胞癌的侵襲轉移.
목적 탐토방광이행세포암(BTCC)상피간질전화(EMT)여핵전록인자차두광단백Q1(FOXQ1)적상관성,이급FOXQ1여방광암림상병리특정지간적관계.방법 선용방광이행세포암수술절제표본65례,매례표본균선용암조직중심、암방조직급기원단정상점막조직대조.채용면역조직화학、역전록-취합매련반응(RT-PCR)화Western blot기술검측각례표본중전화생장인자(TGF)-β1、핵전록인자FOXQ1급상피간질표지물E-개점단백(E-cadhefin)화파형단백(Vimentin)적표체,병분석여림상병리인소지간적관계.결과 (1) FOXQ1재BTCC조직중적표체고우정상점막조직(89.2％비13.8％,P＜0.05),기재기층침윤성암중적표체고우천표성암(97.3％비78.6％),재유림파결전이암중적표체명현고우무림파결전이암(100.0％비86.8％)；TGF-β1재BTCC조직중적표체고우정상점막(76.9％비26.2％,P＜0.05)；E-cadherin재BTCC조직중적표체저우정상점막(27.7％비89.2％,P＜0.05)；Vimentin재BTCC조직중적표체고우정상점막(83.1％비20.0％,P＜0.05).(2) FOXQ1여E-cadherin표체정부상관,여TGF-β1、Vimentin표체정정상관,시EMT적정향조공인자.결론 핵전록인자FOXQ1여방광이행세포암분화화전이유관,가능통과조공EMT래촉진방광이행세포암적침습전이.
Objective To investigate the relationship between transcription factor forkhead box protein Q1 (FOXQ1) and epithelial mesenchymal transition (EMT) in bladder transitional cell carcinoma (BTCC),and the association between FOXQ1 and clinicopathologic features.Methods Sixty-five BTCC specimens surgically resected were selected.For each specimen,the cancerous tissue,peri-cancerous tissue and the remote normal mucosa were analyzed and compared.The expression of FOXQ1,transforming growth factor (TGF)-βl,E-cadherin and Vimentin genes in resected cancer tissues was detected by using reverse transcription-polymerase chain reaction (RT-PCR),Western blotting and immunohistochemistry,and their association with clinicopathologic data was analyzed.Results (1) The expression rate of FOXQ1 was significantly higher in BTCC tissues than in normal tissues (89.2％ vs 13.8％,P ＜0.05),and that in deeply infiltrating group was significantly higher than that in superficially infiltrating group (97.3％ vs 78.6％,P ＜0.05).The level of TGF-β1 was significantly higher in BTCC tissues than in normal tissues (76.9％ vs 26.2％,P ＜0.05).The expression level of E-cadherin in BTCC tissues was significantly lowered in comparison with that in normal bladder epithelium (27.7％ vs 89.2％,P ＜0.05).The expression rate of Vimentin was higher in BTCC tissues than in normal tissues (83.1％ vs 20.0％,P ＜ 0.05) ; (2)The expression of FOXQ1 serving as one new positive control factor of EMT was inversely correlated to Ecadherin,but positively to TGF-β1 and Vimentin.Conclusion FOXQ1 is related to the differentiation and metastasis of BTCC,and may induce EMT to promote BTCC metastasis.
