CAJ | 학술논문

目的观察微小RNA-143(miR-143)对肾细胞癌细胞株A498增殖及凋亡的影响.方法化学合成miR-143模拟物(miR-143 mimics)及阴性对照转染对照序列,用LipofectamineTM 2000脂质体瞬时转染miR-143模拟物及阴性对照转染对照序列至肾细胞癌株A498中,miR-143 mimics和转染对照序列浓度为60 nmol/L,实验分为3组:miR-143 mimics转染组、阴性对照转染组(NC组)、空白对照组(Opti-MEMI无血清培养基培养的A498细胞).采用细胞计数试剂盒(CCK-8)和流式细胞技术等方法检测转染后不同分组肾癌细胞株生长、增殖与凋亡的变化.结果与对照组比较,CCK-8检测结果表明miR-143 mimics转染组细胞增殖抑制明显,且抑制效应于转染48 h后呈现.流式细胞术检测结果显示miR-143 mimics转染组细胞凋亡率增加[(9.10±2.31)％],显著高于阴性对照转染组[(4.23±1.52)％]与空白对照组[(3.43±1.61)％],组间差异有统计学意义(P＜0.05).结论 miR-143模拟物可抑制肾癌细胞的增殖,诱导肾癌细胞凋亡,具有明显的肿瘤抑制效应.
목적 관찰미소RNA-143(miR-143)대신세포암세포주A498증식급조망적영향.방법 화학합성miR-143모의물(miR-143 mimics)급음성대조전염대조서렬,용LipofectamineTM 2000지질체순시전염miR-143모의물급음성대조전염대조서렬지신세포암주A498중,miR-143 mimics화전염대조서렬농도위60 nmol/L,실험분위3조:miR-143 mimics전염조、음성대조전염조(NC조)、공백대조조(Opti-MEMI무혈청배양기배양적A498세포).채용세포계수시제합(CCK-8)화류식세포기술등방법검측전염후불동분조신암세포주생장、증식여조망적변화.결과 여대조조비교,CCK-8검측결과표명miR-143 mimics전염조세포증식억제명현,차억제효응우전염48 h후정현.류식세포술검측결과현시miR-143 mimics전염조세포조망솔증가[(9.10±2.31)％],현저고우음성대조전염조[(4.23±1.52)％]여공백대조조[(3.43±1.61)％],조간차이유통계학의의(P＜0.05).결론 miR-143모의물가억제신암세포적증식,유도신암세포조망,구유명현적종류억제효응.
Objective To observe the effect of microRNA-143 (miR-143) on proliferation and apoptosis of human renal cell carcinoma cell line A498.Methods The miR-143 mimics and negative control transfection sequence were transfected into A498 cells with Lipo2000 liposome,and the concentrations of both miR-143 mimics and negative control transfection sequence were 60 nmol/L.The experiment was divided into three groups:miR-143 mimics-transfected group,negative control-transfected group,blank control group which was cultured with opti-MEMI serum-free culture medium only.After transfection,the cell proliferation and apoptosis were detected by cell counting kit-8 (CCK-8) assay and flow cytometry respectively.Results After transfection of miR-143 mimics,as compared with control group,CCK-8 assay revealed a significant inhibition of proliferation,and inhibition effect occurred at 48th h after transfection.Flow cytometry demonstrated that miR-143 mimics transfection increased apotosis rate [(9.10 ±2.31)％],significantly higher than the negative control group [(4.23 ± 1.52)％] and blank control group [(3.43 ± 1.61) ％],and there was significant difference among the groups (P ＜ 0.05).Conclusion miR-143 could inhibit proliferation and induce apoptosis of renal cell carcinoma cells,suggesting that miR-143 was a tumor suppressor gene in renal cell carcinoma.