CAJ | 학술논문

目的探讨低剂量促红细胞生成素(EPO)减轻肾缺血性损伤后间质纤维化的作用及其机制.方法通过单侧肾缺血再灌注损伤模型造成小鼠患侧肾间质纤维化,小鼠随机分为假手术组、模型组和低剂量EPO给药组,术后28 d处死各组小鼠,取患侧肾做病理学检查,并检测肾组织中层粘连蛋白(LN)、纤维粘连蛋白(FN)、转化生长因子-β1(TGF-β1)的水平.结果给药组肾间质纤维化与模型组比较有所减轻；模型组LN、FN、TGF-β1升高(4.39±0.54、4.99±0.22、4.33±0.29、0.91 ±0.04),EPO给药组明显减低(3.60±0.46、3.85±0.44、3.44±0.52、0.77±0.05),两组间差异有统计学意义(P＜0.01).结论低剂量EPO可以通过影响TGF-β1表达、抑制细胞细胞传导过程以减轻细胞外基质的积聚,从而减轻间质纤维化,发挥肾保护作用.
목적 탐토저제량촉홍세포생성소(EPO)감경신결혈성손상후간질섬유화적작용급기궤제.방법 통과단측신결혈재관주손상모형조성소서환측신간질섬유화,소서수궤분위가수술조、모형조화저제량EPO급약조,술후28 d처사각조소서,취환측신주병이학검사,병검측신조직중층점련단백(LN)、섬유점련단백(FN)、전화생장인자-β1(TGF-β1)적수평.결과 급약조신간질섬유화여모형조비교유소감경；모형조LN、FN、TGF-β1승고(4.39±0.54、4.99±0.22、4.33±0.29、0.91 ±0.04),EPO급약조명현감저(3.60±0.46、3.85±0.44、3.44±0.52、0.77±0.05),량조간차이유통계학의의(P＜0.01).결론 저제량EPO가이통과영향TGF-β1표체、억제세포세포전도과정이감경세포외기질적적취,종이감경간질섬유화,발휘신보호작용.
Objective To evaluate the possible mechanism of low-dose erythropoietin (EPO) alleviating renal interstitial fibrosis after ischemia-reperfusion injury (IRI).Methods Renal interstitial fibrosis was induced by unilateral renal IRI in mice.Mice were randomly divided into three groups:sham-operated,ischemic control and low-dose EPO treatment.They were sacrificed at 28th day after operation.The kidneys were taken for pathological analysis.The expression of laminin (LN),fibronectin (FN) and transforming growth factor-β1 (TGF-β1) in the kidneys was detected by using immunohistochemistry,and TGF-β1 by Western blotting.Results There was remarkable renal interstitial fibrosis in the control group as compared to the EPO group.The levels of LN,FN,and TGF-β1 mRNA and protein were significantly reduced in the EPO group (3.60 ± 0.46,3.85 ± 0.44,3.44 ± 0.52,0.77 ± 0.05) compared to the control group (4.39±0.54,4.99±0.22,4.33 ±0.29,0.91 ±0.04).Conclusion The results suggested low-dose EPO can reduce the accumulation of extracellular matrix through regulating TGF-β1 expression,thereby alleviating renal interstitial ftbrosis and protecting the kidney.