中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
3期
587-589
,共3页
伍信阳%毕学成%何慧婵%江福能%冯自卫%付欣%邓业瀚%钟惟德
伍信暘%畢學成%何慧嬋%江福能%馮自衛%付訢%鄧業瀚%鐘惟德
오신양%필학성%하혜선%강복능%풍자위%부흔%산업한%종유덕
非转移性细胞蛋白1基因%前列腺癌%诊断
非轉移性細胞蛋白1基因%前列腺癌%診斷
비전이성세포단백1기인%전렬선암%진단
Non metastatic cell protein 1 gene%Prostate carcinoma%Diagnosis
目的 检测非转移性细胞蛋白1基因(NME1基因)在前列腺癌组织中的表达,探讨NME1在前列腺癌中的分子机制和临床意义.方法 应用基因芯片筛选出在前列腺癌与前列腺增生组织特异性表达的基因NME1,运用蛋白组学检测前列腺癌组织标本54例、癌旁组织29例、组织中NME1基因结合蛋白差异表达程度.免疫组织化学结合NME1免疫组织化学评分对前列腺癌患者的临床病理参数进行分析.结果 通过基因芯片筛选,可见NME1在前列腺癌组织中表达上调,达2.24倍(P＜0.01).双向差异凝胶电泳(2D-DIGE)技术亦发现相对于癌旁组织NME1基因结合蛋白在前列腺癌组织中表达上调,达2.36倍(P＜0.01).通过免疫组织化学染色,可见NME1在前列腺癌组织中的表达明显强于癌旁组织(P＜0.01),在前列腺癌并转移组织中的表达亦明显强于原位前列腺癌组织(P＜0.01).NME1表达与前列腺癌患者血清前列腺特异抗原(PSA)水平(P＜0.05)、Gleason评分(P＜0.01)和肿瘤TNM分期(P＜0.05)、是否转移(P＜0.01)呈正相关,而与患者年龄无明显相关(P＞0.05).NME1阳性的前列腺癌患者预后不良(Log Rank=11.117,P＜0.01).结论 NME1低表达与肿瘤的恶性程度相关,NME1缺失可能导致恶性肿瘤细胞迅速生长,可结合血清PSA水平对前列腺癌疑似患者进行早期诊断及初步判断肿瘤恶性程度.
目的 檢測非轉移性細胞蛋白1基因(NME1基因)在前列腺癌組織中的錶達,探討NME1在前列腺癌中的分子機製和臨床意義.方法 應用基因芯片篩選齣在前列腺癌與前列腺增生組織特異性錶達的基因NME1,運用蛋白組學檢測前列腺癌組織標本54例、癌徬組織29例、組織中NME1基因結閤蛋白差異錶達程度.免疫組織化學結閤NME1免疫組織化學評分對前列腺癌患者的臨床病理參數進行分析.結果 通過基因芯片篩選,可見NME1在前列腺癌組織中錶達上調,達2.24倍(P＜0.01).雙嚮差異凝膠電泳(2D-DIGE)技術亦髮現相對于癌徬組織NME1基因結閤蛋白在前列腺癌組織中錶達上調,達2.36倍(P＜0.01).通過免疫組織化學染色,可見NME1在前列腺癌組織中的錶達明顯彊于癌徬組織(P＜0.01),在前列腺癌併轉移組織中的錶達亦明顯彊于原位前列腺癌組織(P＜0.01).NME1錶達與前列腺癌患者血清前列腺特異抗原(PSA)水平(P＜0.05)、Gleason評分(P＜0.01)和腫瘤TNM分期(P＜0.05)、是否轉移(P＜0.01)呈正相關,而與患者年齡無明顯相關(P＞0.05).NME1暘性的前列腺癌患者預後不良(Log Rank=11.117,P＜0.01).結論 NME1低錶達與腫瘤的噁性程度相關,NME1缺失可能導緻噁性腫瘤細胞迅速生長,可結閤血清PSA水平對前列腺癌疑似患者進行早期診斷及初步判斷腫瘤噁性程度.
목적 검측비전이성세포단백1기인(NME1기인)재전렬선암조직중적표체,탐토NME1재전렬선암중적분자궤제화림상의의.방법 응용기인심편사선출재전렬선암여전렬선증생조직특이성표체적기인NME1,운용단백조학검측전렬선암조직표본54례、암방조직29례、조직중NME1기인결합단백차이표체정도.면역조직화학결합NME1면역조직화학평분대전렬선암환자적림상병리삼수진행분석.결과 통과기인심편사선,가견NME1재전렬선암조직중표체상조,체2.24배(P＜0.01).쌍향차이응효전영(2D-DIGE)기술역발현상대우암방조직NME1기인결합단백재전렬선암조직중표체상조,체2.36배(P＜0.01).통과면역조직화학염색,가견NME1재전렬선암조직중적표체명현강우암방조직(P＜0.01),재전렬선암병전이조직중적표체역명현강우원위전렬선암조직(P＜0.01).NME1표체여전렬선암환자혈청전렬선특이항원(PSA)수평(P＜0.05)、Gleason평분(P＜0.01)화종류TNM분기(P＜0.05)、시부전이(P＜0.01)정정상관,이여환자년령무명현상관(P＞0.05).NME1양성적전렬선암환자예후불량(Log Rank=11.117,P＜0.01).결론 NME1저표체여종류적악성정도상관,NME1결실가능도치악성종류세포신속생장,가결합혈청PSA수평대전렬선암의사환자진행조기진단급초보판단종류악성정도.
Objective To explore the role of non metastatic cell protein 1 (NME1) in diagnosing prostate cancer (PCa) and judging early metastasis of PCa.Methods The gene chip was used to screen NME1 in PCa and benign prostatic hyperplasia (BPH) tissues,the differential expression of NME1 prostein in PCa and adjacent tissues was analyzed by using fluorescence 2-D difference gel electrophoresis (2DDIGE) technology,and validated by using immunohistochemistry (IHC).The NME1 IHC score and clinicopathological parameters of PCa patients were analyzed.Results Relative to BPH tissues the expression of NME1 was down-regulated in PCa tissues by gene chip screening (P ＜ 0.01),and relative to adjacent tissues the same phenomenon was observed in PCa tissues by using 3D-DIGE technology (P ＜0.01).The expression of NME1 was negatively correlated with serum PSA levels (P ＜ 0.05),Gleason score (P ＜0.01) and TNM stage (P ＜ 0.05),positively correlated with metastasis (P ＜ 0.01),but not correlated with age (P ＞ 0.05).NME1-positive PCa patients had a lower overall survival rate (Log Rank =11.117,P ＜0.01),but there was no significant difference in biochemical recurrence rate between NMEl-negative and NMEl-positive PCa patients (Log Rank =0.247,P ＞ 0.05).Conclusion The low expression of NME1 is closely related with the malignancy of PCa,and the loss of NME1 may promote tumor cell growth.Serum PSA levels in combination with NME1 levels can be used to diagnose PCa early and judge the degree of the malignancy of PCa.The high expression of NME1 may promote PCa metastasis.
