CAJ | 학술논문

目的探讨周期性应力下大鼠髓核细胞中整合素的表达和意义.方法体外分离培养大鼠髓核细胞,接种于玻片上,随机分为对照组和加压组.加压组在0～ 200 kPa,0.1 Hz的周期性应力下培养6h.使用荧光实时定量聚合酶链反应(FQ-PCR)检测两组细胞中整合素不同亚基、Ⅱ型胶原和蛋白多糖的基因表达.结果加压组Ⅱ型胶原和蛋白多糖的基因表达明显增加,分别为对照组的5.04和4.52倍,同时整合素α1亚基的表达为对照组的1.71倍(P＜0.05).结论周期性压力对髓核细胞的代谢具有促进作用,整合素α1亚基在压力传导中起信号传递作用.
목적 탐토주기성응력하대서수핵세포중정합소적표체화의의.방법 체외분리배양대서수핵세포,접충우파편상,수궤분위대조조화가압조.가압조재0～ 200 kPa,0.1 Hz적주기성응력하배양6h.사용형광실시정량취합매련반응(FQ-PCR)검측량조세포중정합소불동아기、Ⅱ형효원화단백다당적기인표체.결과 가압조Ⅱ형효원화단백다당적기인표체명현증가,분별위대조조적5.04화4.52배,동시정합소α1아기적표체위대조조적1.71배(P＜0.05).결론 주기성압력대수핵세포적대사구유촉진작용,정합소α1아기재압력전도중기신호전체작용.
Objective To evaluate the gene expression of different integrinsubunits in rat nucleus pulposus cells under periodic mechanical stress.Methods Nucleus pulposus cells obtained from intervetebral disc cells of 4-week-old rats were planted on glass slides.All slides were randomized into two groups:the control group and the stress group.The cells in the stress group were cultured under periodic mechanical stress (0-200 kPa,0.1 Hz) for 6 h,then the gene expression of type Ⅱ collagen,aggrecan and different integrin subunits was detected by using real-time fluorescent quantitative polymerase chain reaction (FQ-PCR).Results The gene expression levels of typeⅡ collagen,aggrecan and integin α1 were upgraded to 5.04,4.52 and 1.71 times higher in stress group than in control group respectively (P ＜ 0.05).Conclusion Periodic mechanical stress can promote the metabolism of rat nucleus pulposus cells.Integrin α1 may play important roles in the mechanical transductions.