中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
3期
612-614
,共3页
胶质细胞源性神经营养因子%神经病理性疼痛%Fos蛋白
膠質細胞源性神經營養因子%神經病理性疼痛%Fos蛋白
효질세포원성신경영양인자%신경병이성동통%Fos단백
Glial cell line-derived neurotrophic factor%Neuropathic pain%Fos protein
目的 观察蛛网膜下腔注射胶质细胞源性神经营养因子(GDNF)对脊神经结扎(SNL)大鼠脊髓背角Fos蛋白的影响.方法 将L5-6脊神经结扎SD雄性大鼠随机分为对照组、假手术组、单纯SNL组(蛛网膜下腔注射生理盐水)、GDNF治疗组(蛛网膜下腔注射GDNF).每组又根据处死大鼠的时间不同各分为3个亚组:术后3、7、14 d.于大鼠脊神经结扎后不同时间点取脊神经结扎侧脊髓,测定脊髓背角处Fos蛋白的表达.结果 与对照组和假手术组比较,SNL组脊髓背角Fos蛋白表达在术后3d(分别为1.00±0.00、1.08±0.12、4.62±0.52)开始增高,一直持续至术后14 d(分别为1.00±0.00、1.15 ±0.21、4.43±0.84)(P＜0.01或P＜0.05)；GDNF组Fos蛋白表达(1.82±0.51)明显低于SNL组,持续至术后14 d(1.61±0.26)(P＜0.01或P＜0.05).结论 大鼠脊髓背角中Fos蛋白参与了SNL所致神经病理性疼痛的发生机制,蛛网膜下腔注射GDNF大鼠减轻神经病理性疼痛机制与其抑制脊髓背角Fos蛋白表达有关.
目的 觀察蛛網膜下腔註射膠質細胞源性神經營養因子(GDNF)對脊神經結扎(SNL)大鼠脊髓揹角Fos蛋白的影響.方法 將L5-6脊神經結扎SD雄性大鼠隨機分為對照組、假手術組、單純SNL組(蛛網膜下腔註射生理鹽水)、GDNF治療組(蛛網膜下腔註射GDNF).每組又根據處死大鼠的時間不同各分為3箇亞組:術後3、7、14 d.于大鼠脊神經結扎後不同時間點取脊神經結扎側脊髓,測定脊髓揹角處Fos蛋白的錶達.結果 與對照組和假手術組比較,SNL組脊髓揹角Fos蛋白錶達在術後3d(分彆為1.00±0.00、1.08±0.12、4.62±0.52)開始增高,一直持續至術後14 d(分彆為1.00±0.00、1.15 ±0.21、4.43±0.84)(P＜0.01或P＜0.05)；GDNF組Fos蛋白錶達(1.82±0.51)明顯低于SNL組,持續至術後14 d(1.61±0.26)(P＜0.01或P＜0.05).結論 大鼠脊髓揹角中Fos蛋白參與瞭SNL所緻神經病理性疼痛的髮生機製,蛛網膜下腔註射GDNF大鼠減輕神經病理性疼痛機製與其抑製脊髓揹角Fos蛋白錶達有關.
목적 관찰주망막하강주사효질세포원성신경영양인자(GDNF)대척신경결찰(SNL)대서척수배각Fos단백적영향.방법 장L5-6척신경결찰SD웅성대서수궤분위대조조、가수술조、단순SNL조(주망막하강주사생리염수)、GDNF치료조(주망막하강주사GDNF).매조우근거처사대서적시간불동각분위3개아조:술후3、7、14 d.우대서척신경결찰후불동시간점취척신경결찰측척수,측정척수배각처Fos단백적표체.결과 여대조조화가수술조비교,SNL조척수배각Fos단백표체재술후3d(분별위1.00±0.00、1.08±0.12、4.62±0.52)개시증고,일직지속지술후14 d(분별위1.00±0.00、1.15 ±0.21、4.43±0.84)(P＜0.01혹P＜0.05)；GDNF조Fos단백표체(1.82±0.51)명현저우SNL조,지속지술후14 d(1.61±0.26)(P＜0.01혹P＜0.05).결론 대서척수배각중Fos단백삼여료SNL소치신경병이성동통적발생궤제,주망막하강주사GDNF대서감경신경병이성동통궤제여기억제척수배각Fos단백표체유관.
Objective To study the effect of glial cell line-derived neurotrophic factor (GDNF)on Fos protein expression in the spinal dorsal horn of neuropathic pain rat model.Methods Adult Sprague-Dawley male rats were randomly divided into normal control group,sham operation group,spinal nerve ligation (SNL) group and SNL combined with treatment of GDNF group.Every group was divided into three sub-groups:SNL 3,7 and 14 days (n =10 in each group).Fos protein expression in the spinal dorsal horn was examined by using inununohistochemistry and Western blotting.Results Compared with normal control and sham operation groups,the Fos protein expression in the spinal dorsal horn of the rats in SNL group was increased significantly (1.00 ± 0.00,1.08 ± 0.12,and 4.62 ± 0.52 respectively).As compared with the rats in SNL group,there was an apparent decrease in GDNF treatment group three days later (1.82 ± 0.51).Conclusion Fos protein in the spinal dorsal horn of rats was involved in the mechanism of neuropathic pain.Intrathecal administration of GDNF could inhibit Fos protein expression in the spinal dorsal horn.
