CAJ | 학술논문

目的建立一种改良的Wistar大鼠梗阻性黄疸肝切除术后肝衰竭模型.方法通过切断胆总管建立梗阻性黄疸模型,探索最佳梗阻时间.在梗阻14 d后,行胆道内引流,分离出70％肝脏分别阻断0、15、30、45 min,切除未行阻断的余肝,建立肝衰模型,观察生化指标、病理改变及生存率.结果梗阻14d组更加适合下一步造模.随着阻断时间的延长肝功能进行性下降,各组间差异有统计学意义(P＜0.05),其中30 min组24h死亡率达80％,有典型肝衰症状,组织病理学符合肝衰特点.结论对梗阻性黄疸大鼠行肝脏血流阻断及肝切除可建立能较大程度模拟临床过程的外科型肝衰竭模型.
목적 건립일충개량적Wistar대서경조성황달간절제술후간쇠갈모형.방법 통과절단담총관건립경조성황달모형,탐색최가경조시간.재경조14 d후,행담도내인류,분리출70％간장분별조단0、15、30、45 min,절제미행조단적여간,건립간쇠모형,관찰생화지표、병리개변급생존솔.결과 경조14d조경가괄합하일보조모.수착조단시간적연장간공능진행성하강,각조간차이유통계학의의(P＜0.05),기중30 min조24h사망솔체80％,유전형간쇠증상,조직병이학부합간쇠특점.결론 대경조성황달대서행간장혈류조단급간절제가건립능교대정도모의림상과정적외과형간쇠갈모형.
Objective To establish a modified operative method-induced postoperative liver failure in Wistar rats.Methods An obstructive jaundice model with bile duct cut off in rats was established to study the optimum obstruction time.Besides,a rat model of liver failure was established.After obstruction for 14 days,the rats were subjected to internal biliary drainage.Afterwards,the vessel of the left and middle lobe of liver(occupied 70％ of the total volume) for different time,then resected the other lobes of liver without ischemia.To observe and compare the biochemical indexes,the histopathology,and the survival time.Results 14 d group is more suitable for the next treatment.The liver function of the rats were decreased with prolonging the time of ischemia,the differences between groups were significant (P ＜ 0.05).The mortality was as high as 80％ after 24 hours in 30 min group,and this group had the typical symptoms of liver failure and the pathology was significant Conclusion The experimental model of postoperative liver failure could be established by partially hepatectomized after hepatic devascularization in Wistar rats with obstructive jaundice.It showed similar clinical pathophysiological changes in hunman beings.