中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
661-663
,共3页
赵向旺%刘春萍%彭功玲%田野%郭雅文
趙嚮旺%劉春萍%彭功玲%田野%郭雅文
조향왕%류춘평%팽공령%전야%곽아문
乳腺癌%侵袭%转移%免疫
乳腺癌%侵襲%轉移%免疫
유선암%침습%전이%면역
Breast carcinoma%Invasiveness%Metastasis%Immune
目的 观察乳腺癌细胞株MCF-7和MDA-MB-231侵袭能力和免疫逃逸能力的差异.方法 以Transwell小室进行人工基底膜侵袭实验检测1.0×105个/孔MCF-7细胞和MDA-MB-231细胞侵袭能力的差异；不同浓度混合淋巴细胞与5.0×103个/孔乳腺癌细胞株共培养检测MCF-7细胞和MDA-MB-231细胞免疫逃逸能力的差异.结果 MDA-MB-231细胞侵袭细胞数量高于MCF-7细胞侵袭细胞数量(86.64±7.13比12.27 ±2.53),差异有统计学意义(P＜O.05)；与2.5×104个/孔、5.0×104个/孔、1.O×1 05个/孔、2.0×1O5个/孔混合淋巴细胞共培养后,MDA-MB-231细胞存活率高于MCF-7细胞存活率[(1.31±0.04)％比(0.97±0.01)％；(1.31±0.03)％比(0.80±0.01)％；(1.43±0.00)％比(0.60±0.01)％；(1.57±0.09)％比(0.59±0.01)％],差异有统计学意义(P＜0.05).结论 MDA-MB-231细胞株较MCF-7细胞株有更强的侵袭能力和免疫逃逸能力.
目的 觀察乳腺癌細胞株MCF-7和MDA-MB-231侵襲能力和免疫逃逸能力的差異.方法 以Transwell小室進行人工基底膜侵襲實驗檢測1.0×105箇/孔MCF-7細胞和MDA-MB-231細胞侵襲能力的差異；不同濃度混閤淋巴細胞與5.0×103箇/孔乳腺癌細胞株共培養檢測MCF-7細胞和MDA-MB-231細胞免疫逃逸能力的差異.結果 MDA-MB-231細胞侵襲細胞數量高于MCF-7細胞侵襲細胞數量(86.64±7.13比12.27 ±2.53),差異有統計學意義(P＜O.05)；與2.5×104箇/孔、5.0×104箇/孔、1.O×1 05箇/孔、2.0×1O5箇/孔混閤淋巴細胞共培養後,MDA-MB-231細胞存活率高于MCF-7細胞存活率[(1.31±0.04)％比(0.97±0.01)％；(1.31±0.03)％比(0.80±0.01)％；(1.43±0.00)％比(0.60±0.01)％；(1.57±0.09)％比(0.59±0.01)％],差異有統計學意義(P＜0.05).結論 MDA-MB-231細胞株較MCF-7細胞株有更彊的侵襲能力和免疫逃逸能力.
목적 관찰유선암세포주MCF-7화MDA-MB-231침습능력화면역도일능력적차이.방법 이Transwell소실진행인공기저막침습실험검측1.0×105개/공MCF-7세포화MDA-MB-231세포침습능력적차이；불동농도혼합림파세포여5.0×103개/공유선암세포주공배양검측MCF-7세포화MDA-MB-231세포면역도일능력적차이.결과 MDA-MB-231세포침습세포수량고우MCF-7세포침습세포수량(86.64±7.13비12.27 ±2.53),차이유통계학의의(P＜O.05)；여2.5×104개/공、5.0×104개/공、1.O×1 05개/공、2.0×1O5개/공혼합림파세포공배양후,MDA-MB-231세포존활솔고우MCF-7세포존활솔[(1.31±0.04)％비(0.97±0.01)％；(1.31±0.03)％비(0.80±0.01)％；(1.43±0.00)％비(0.60±0.01)％；(1.57±0.09)％비(0.59±0.01)％],차이유통계학의의(P＜0.05).결론 MDA-MB-231세포주교MCF-7세포주유경강적침습능력화면역도일능력.
Objective To study the differences of invasiveness and immune evasion between MCF-7 and MDA-MB-231 in vitro.Methods Transwell was used to analyze the difference of invasiveness between 100 thousands per hole MDA-MB-231 and MCF-7; Co-culturing with 5 thousands per hole breast cells mixed by various concentrations of lymphocytes was used to observe the difference of immune evasion between MDA-MB-231 and MCF-7.Results MDA-MB-231 invaded more cells than MCF-7 (86.64 ±7.13 vs 12.27 ±2.53,P＜0.05) ; The survival rate of MDA-MB-231 was higher than that of MCF-7 coculturing with 25 thousands per hole,50 thousands per hole,100 thousands per hole,200 thousands per holelymphocytes [(1.31 ±0.04)％ vs (0.97 ±0.01)％,P＜0.05; (1.31 ±0.03)％ vs (0.80 ±0.01)％,P＜0.05; (1.43 ±0.00)％ vs (0.60 ±0.01)％,P＜0.05; (1.57 ±0.09)％ vs (0.59 ±0.01) ％,P ＜ 0.05].Conclusion Our results suggest that MDA-MB-231 cells display stronger invasiveness and immune evasion.
