中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
691-693
,共3页
谢松涛%白晓智%胡晓龙%石继红%朱雄翔%汤朝武%胡大海
謝鬆濤%白曉智%鬍曉龍%石繼紅%硃雄翔%湯朝武%鬍大海
사송도%백효지%호효룡%석계홍%주웅상%탕조무%호대해
乳香%成纤维细胞%创面愈合%细胞增殖
乳香%成纖維細胞%創麵愈閤%細胞增殖
유향%성섬유세포%창면유합%세포증식
Frankincense%Fibroblasts%Wound healing%Cytoproliferation
目的 观察乳香活性提取物对人皮肤成纤维细胞(FB)生物学特性的影响,探讨其促进创面愈合的机制.方法 分离培养人正常FB,按照随机数字表法分为乳香活性提取物不同浓度组(5×10-4、5×10-3、5×10-2、5×10-1g/L),含0.25％新生小牛血清的DMEM培养液为对照组.采用噻唑蓝(MTT)比色法检测不同浓度乳香活性提取物对体外培养的FB增殖作用的影响.通过流式细胞仪、实时定量聚合酶链反应(Real-time PCR)分别检测乳香活性提取物最适浓度培养条件下FB的细胞周期变化以及Ⅰ、Ⅲ型胶原mRNA的表达.结果 乳香活性提取物在5×10-3、5×10-2 g/L浓度范围,可显著促进FB增殖,测得吸光度(A)值分别为0.312±0.035、0.366±0.051,与对照组(0.257±0.029)比较差异有统计学意义(t值分别为2.67、3.89,P＜0.01),其中在5×10-2 g/L浓度时测得A值最高,促增殖作用最为显著,为乳香活性提取物促进FB增殖的最适浓度.此浓度条件下乳香活性提取物可明显促进FB通过G1/S及S/G2期限制点,S期(18.30±3.44)及G2/M (9.60±1.26)期细胞与对照组(10.60±1.72、5.30 ±0.68)比较明显增多,G0/G1期细胞(71.80±3.92)与对照组(83.60±6.85)比较明显减少(t值分别为:14.44、8.97、3.52,P＜0.05).5×10-2 g/L乳香活性提取物组与对照组比较Ⅰ、Ⅲ型胶原mRNA表达明显上调分别为2.416±0.247比1.000±0.081和4.681±0.126比1.000±0.072,差异有统计学意义(t=4.87、15.31,P＜0.01).结论 乳香活性提取物能显著促进FB增殖,加快 FB 周期进程,同时可上调Ⅰ、Ⅲ型胶原 mRNA 的表达,可能与乳香促进创面愈合的机制有关.
目的 觀察乳香活性提取物對人皮膚成纖維細胞(FB)生物學特性的影響,探討其促進創麵愈閤的機製.方法 分離培養人正常FB,按照隨機數字錶法分為乳香活性提取物不同濃度組(5×10-4、5×10-3、5×10-2、5×10-1g/L),含0.25％新生小牛血清的DMEM培養液為對照組.採用噻唑藍(MTT)比色法檢測不同濃度乳香活性提取物對體外培養的FB增殖作用的影響.通過流式細胞儀、實時定量聚閤酶鏈反應(Real-time PCR)分彆檢測乳香活性提取物最適濃度培養條件下FB的細胞週期變化以及Ⅰ、Ⅲ型膠原mRNA的錶達.結果 乳香活性提取物在5×10-3、5×10-2 g/L濃度範圍,可顯著促進FB增殖,測得吸光度(A)值分彆為0.312±0.035、0.366±0.051,與對照組(0.257±0.029)比較差異有統計學意義(t值分彆為2.67、3.89,P＜0.01),其中在5×10-2 g/L濃度時測得A值最高,促增殖作用最為顯著,為乳香活性提取物促進FB增殖的最適濃度.此濃度條件下乳香活性提取物可明顯促進FB通過G1/S及S/G2期限製點,S期(18.30±3.44)及G2/M (9.60±1.26)期細胞與對照組(10.60±1.72、5.30 ±0.68)比較明顯增多,G0/G1期細胞(71.80±3.92)與對照組(83.60±6.85)比較明顯減少(t值分彆為:14.44、8.97、3.52,P＜0.05).5×10-2 g/L乳香活性提取物組與對照組比較Ⅰ、Ⅲ型膠原mRNA錶達明顯上調分彆為2.416±0.247比1.000±0.081和4.681±0.126比1.000±0.072,差異有統計學意義(t=4.87、15.31,P＜0.01).結論 乳香活性提取物能顯著促進FB增殖,加快 FB 週期進程,同時可上調Ⅰ、Ⅲ型膠原 mRNA 的錶達,可能與乳香促進創麵愈閤的機製有關.
목적 관찰유향활성제취물대인피부성섬유세포(FB)생물학특성적영향,탐토기촉진창면유합적궤제.방법 분리배양인정상FB,안조수궤수자표법분위유향활성제취물불동농도조(5×10-4、5×10-3、5×10-2、5×10-1g/L),함0.25％신생소우혈청적DMEM배양액위대조조.채용새서람(MTT)비색법검측불동농도유향활성제취물대체외배양적FB증식작용적영향.통과류식세포의、실시정량취합매련반응(Real-time PCR)분별검측유향활성제취물최괄농도배양조건하FB적세포주기변화이급Ⅰ、Ⅲ형효원mRNA적표체.결과 유향활성제취물재5×10-3、5×10-2 g/L농도범위,가현저촉진FB증식,측득흡광도(A)치분별위0.312±0.035、0.366±0.051,여대조조(0.257±0.029)비교차이유통계학의의(t치분별위2.67、3.89,P＜0.01),기중재5×10-2 g/L농도시측득A치최고,촉증식작용최위현저,위유향활성제취물촉진FB증식적최괄농도.차농도조건하유향활성제취물가명현촉진FB통과G1/S급S/G2기한제점,S기(18.30±3.44)급G2/M (9.60±1.26)기세포여대조조(10.60±1.72、5.30 ±0.68)비교명현증다,G0/G1기세포(71.80±3.92)여대조조(83.60±6.85)비교명현감소(t치분별위:14.44、8.97、3.52,P＜0.05).5×10-2 g/L유향활성제취물조여대조조비교Ⅰ、Ⅲ형효원mRNA표체명현상조분별위2.416±0.247비1.000±0.081화4.681±0.126비1.000±0.072,차이유통계학의의(t=4.87、15.31,P＜0.01).결론 유향활성제취물능현저촉진FB증식,가쾌 FB 주기진정,동시가상조Ⅰ、Ⅲ형효원 mRNA 적표체,가능여유향촉진창면유합적궤제유관.
Objective To observe the effects of Frankincense extracts on the biological characteristics of human dermal fibroblasts (FBs) in vitro and explore the possible therapeutic mechanism for wound healing.Methods The optimal concentration of Frankincense extracts was identified by analysing proliferation activity of human normal FBs that treated with different concentration of Frankincense extracts through thiazole blue (MTT) colorimetric assay.Cell cycle,collagen Ⅰ and collagen Ⅲ mRNA levels in FBs treated with the optimal concentration of Frankincense extracts were detected by using flow cytometry (FCM) and real-time polymerase chain reaction (PCR).Results At concentrations of 5 × 10-3 g/L to 5 × 10-2 g/L,the Frankincense extracts significantly enhanced the proliferation of FBs.The most significant concentration was 5 × 10-2 g/L(t =3.89,P ＜0.01),at which an increased percentage of G1 to S and S to G2 phase cells as well as an increased level of collagen Ⅰ and collagen Ⅲ mRNA were obtained compared to the control group.Conclusion Frankincense extracts can notably promote the proliferation of FB and accelerating the cell cycle of FB as well as up-regulating the expression of collagen Ⅰ and collagen Ⅲ,which may enhance the process of wound healing.
