CAJ | 학술논문

目的制备1种高分子材料聚乙烯亚胺(PEI)修饰的磁性金纳米粒子作为新型基因载体,观察其表征及结合DNA的能力.方法应用原位合成法合成稳定的复合磁性金纳米微粒(Au-MP-PEI),将适量FeCl3和FeSO4制备纳米磁颗粒(MP-CA)分散液,加入适量PEI溶液及氯金酸(Au-Cl4·3H2O)溶液沸腾制得Au-MP-PEI;通过透射电镜观察磁性金纳米粒形态,并检测该纳米粒的粒径及电位分析；采用原液及稀释1倍的纳米载体进行磁性金纳米载体-DNA的结合实验.结果对PEI包敷的纳米磁颗粒(MP-PEI)做磷钨酸染色后电镜扫描可见每1个团聚体都有数个纳米颗粒构成,笼罩在薄薄一层灰黑色分子下,大小在90 nm左右,而在磁性金纳米颗粒电镜照片中磁颗粒表面可见较多黑色点状颗粒,此即为合成的胶体金.通过不同浓度的金磁纳米载体凝胶阻滞电泳证明磁性金纳米原液能将DNA完全结合.结论成功制备粒径均一的磁性金纳米粒子作为新型基因载体,并且凝胶电泳实验证明该基因载体对DNA具有完全结合的能力.
목적 제비1충고분자재료취을희아알(PEI)수식적자성금납미입자작위신형기인재체,관찰기표정급결합DNA적능력.방법 응용원위합성법합성은정적복합자성금납미미립(Au-MP-PEI),장괄량FeCl3화FeSO4제비납미자과립(MP-CA)분산액,가입괄량PEI용액급록금산(Au-Cl4·3H2O)용액비등제득Au-MP-PEI;통과투사전경관찰자성금납미립형태,병검측해납미립적립경급전위분석；채용원액급희석1배적납미재체진행자성금납미재체-DNA적결합실험.결과 대PEI포부적납미자과립(MP-PEI)주린오산염색후전경소묘가견매1개단취체도유수개납미과립구성,롱조재박박일층회흑색분자하,대소재90 nm좌우,이재자성금납미과립전경조편중자과립표면가견교다흑색점상과립,차즉위합성적효체금.통과불동농도적금자납미재체응효조체전영증명자성금납미원액능장DNA완전결합.결론 성공제비립경균일적자성금납미입자작위신형기인재체,병차응효전영실험증명해기인재체대DNA구유완전결합적능력.
Objective To synthesize macromolecule materials polyethyleneimine (PEl) modified magnetic gold nano-particles,then study their characteristics and DNA binding ability.Methods The stable magnetic gold nano-particles (Au-MP-PEI) were synthesized with in situ synthesis,and observed under a transmission electron microscope (TEM).The particle size and potential were analyzed.DNA binding test was done with magnetic gold nano-particle vector in original nano-vector fluid and nano-vector fluid diluted one time.Results The magnetic nano-particles wraped by PEI (MP-PEI) were scanned under the TEM after phosphotungstic acid stain,and it was found that every aggregate was formed by several nanoparticles,enveloped by a thin layer of greyblack molecules,with a diameter of about 90 nm,and electron microscopic scanning of magnetic gold nano-particle showed more black punctiform particles,synthesized colloidal gold.Gel retardation electrophoresis with different concentrations of magnetic gold nano-particles revealed that the original fluid of magnetic gold nano-particles could completely bind DNA.Conclusion A new gene carrier which is uniform particle-size magnetic gold nano-particle is successfully,and gel electrophoresis proves its complete DNA binding ability as well.