中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
725-727
,共3页
王育斌%赵运海%严若华%刘智明
王育斌%趙運海%嚴若華%劉智明
왕육빈%조운해%엄약화%류지명
血管性痴呆%白细胞介素-32%认知功能障碍
血管性癡呆%白細胞介素-32%認知功能障礙
혈관성치태%백세포개소-32%인지공능장애
Vascular dementia%Interleukin-32%Cognitive impairment
目的 观察血管性痴呆模型大鼠白细胞介素-32(IL-32)水平的变化及其对大鼠认知功能的影响.方法 SD雄性大鼠30只,随机分为模型组和对照组,每组15只,模型组大鼠双侧颈总动脉永久性结扎(2VO),对照组大鼠仅分离双侧颈总动脉而不结扎；酶联免疫吸附试验(ELISA)检测术后第3、7、14、28天大鼠血清中IL-32的水平,采用Morris水迷宫评价大鼠的认知功能；术后第28天处死大鼠,取脑组织HE染色后镜下观察海马CA1区的变化并用免疫组织化学方法检测IL-32表达.结果 术后第3、7、14、28天大鼠血清中IL-32水平,模型组分别为(93.44 ±25.53)、(121.69 ±23.14)、(171.69±21.65)和(121.66±23.24) ng/L,对照组分别为(51.69±12.16)、(54.29±11.12)、(55.19 ±10.08)和(54.79±11.18) ng/L,模型组均显著高于对照组(P＜0.05)；水迷宫定位航行实验中潜伏期,模型组比对照组明显延长[(14.88 ±4.23)比(7.15±1.31)s,P＜0.05],空间探索实验,模型组较对照组穿越目标次数明显减少[(6.0±1.2)比(8.0±1.5),P＜0.05]；苏木素-伊红(HE)染色后可见模型组大鼠海马CA1区神经细胞数目减少、排列紊乱,神经细胞核深染、固缩,并可见大量不规则形胶质细胞；免疫组织化学检测结果显示模型组阳性面积率高于对照组[(0.4447 ±0.0555)％比(0.1175 ±0.0114)％,P＜0.05].结论 IL-32在血管性痴呆模型大鼠高表达且水平与认知损伤的程度相关,其机制可能与其触发炎性反应有关.
目的 觀察血管性癡呆模型大鼠白細胞介素-32(IL-32)水平的變化及其對大鼠認知功能的影響.方法 SD雄性大鼠30隻,隨機分為模型組和對照組,每組15隻,模型組大鼠雙側頸總動脈永久性結扎(2VO),對照組大鼠僅分離雙側頸總動脈而不結扎；酶聯免疫吸附試驗(ELISA)檢測術後第3、7、14、28天大鼠血清中IL-32的水平,採用Morris水迷宮評價大鼠的認知功能；術後第28天處死大鼠,取腦組織HE染色後鏡下觀察海馬CA1區的變化併用免疫組織化學方法檢測IL-32錶達.結果 術後第3、7、14、28天大鼠血清中IL-32水平,模型組分彆為(93.44 ±25.53)、(121.69 ±23.14)、(171.69±21.65)和(121.66±23.24) ng/L,對照組分彆為(51.69±12.16)、(54.29±11.12)、(55.19 ±10.08)和(54.79±11.18) ng/L,模型組均顯著高于對照組(P＜0.05)；水迷宮定位航行實驗中潛伏期,模型組比對照組明顯延長[(14.88 ±4.23)比(7.15±1.31)s,P＜0.05],空間探索實驗,模型組較對照組穿越目標次數明顯減少[(6.0±1.2)比(8.0±1.5),P＜0.05]；囌木素-伊紅(HE)染色後可見模型組大鼠海馬CA1區神經細胞數目減少、排列紊亂,神經細胞覈深染、固縮,併可見大量不規則形膠質細胞；免疫組織化學檢測結果顯示模型組暘性麵積率高于對照組[(0.4447 ±0.0555)％比(0.1175 ±0.0114)％,P＜0.05].結論 IL-32在血管性癡呆模型大鼠高錶達且水平與認知損傷的程度相關,其機製可能與其觸髮炎性反應有關.
목적 관찰혈관성치태모형대서백세포개소-32(IL-32)수평적변화급기대대서인지공능적영향.방법 SD웅성대서30지,수궤분위모형조화대조조,매조15지,모형조대서쌍측경총동맥영구성결찰(2VO),대조조대서부분리쌍측경총동맥이불결찰；매련면역흡부시험(ELISA)검측술후제3、7、14、28천대서혈청중IL-32적수평,채용Morris수미궁평개대서적인지공능；술후제28천처사대서,취뇌조직HE염색후경하관찰해마CA1구적변화병용면역조직화학방법검측IL-32표체.결과 술후제3、7、14、28천대서혈청중IL-32수평,모형조분별위(93.44 ±25.53)、(121.69 ±23.14)、(171.69±21.65)화(121.66±23.24) ng/L,대조조분별위(51.69±12.16)、(54.29±11.12)、(55.19 ±10.08)화(54.79±11.18) ng/L,모형조균현저고우대조조(P＜0.05)；수미궁정위항행실험중잠복기,모형조비대조조명현연장[(14.88 ±4.23)비(7.15±1.31)s,P＜0.05],공간탐색실험,모형조교대조조천월목표차수명현감소[(6.0±1.2)비(8.0±1.5),P＜0.05]；소목소-이홍(HE)염색후가견모형조대서해마CA1구신경세포수목감소、배렬문란,신경세포핵심염、고축,병가견대량불규칙형효질세포；면역조직화학검측결과현시모형조양성면적솔고우대조조[(0.4447 ±0.0555)％비(0.1175 ±0.0114)％,P＜0.05].결론 IL-32재혈관성치태모형대서고표체차수평여인지손상적정도상관,기궤제가능여기촉발염성반응유관.
Objective To observe the expression of interleukin (IL)-32 and its effects on cognitive function in rats with vascular dementia (VD).Methods Thirty male SD rats were divided randomly into model group and control group (n =15 each).The bilateral common carotid artery of model group was occluded (two-vessel occlusion,2VO),and rats in control group were subjected to sham-operation but without 2VO.At 1st,3rd,7th,14th,and 28th day after operation,the IL-32 levels were determined by using enzyme linked immunosorbent assay (ELISA),and the cognitive function was evaluated.Results The IL-32 levels in model group were significantly higher than those in control group at 3rd,7th,14th,and 28th day after operation [(93.44 ± 25.53),(121.69 ± 23.14),(171.69 ± 21.65),(121.66 ± 23.24) vs.(51.69±12.16),(54.29±11.12),(55.19±10.08),(54.79±11.18) ng/L,P＜0.05].Histologically,neurocytes in the hippocampus CA1 region in VD rats were decreased,arranged irregularly and had small hyperchromatic nuclei.Moreover,a number of the irregular glial cells were found.Immunohistochemically,the positive expression rate in model group was significantly greater than in control group [(0.4447 ±0.0555)％ vs.(0.1175 ±0.0114)％,P＜0.05].Conclusion The IL-32 was highly expressed in VD model rats and its level was associated with the extent of cognitive impairment,which may be related to inflammatory response.
