中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
735-737
,共3页
陈钢%刘相燕%王洲%刘凡英
陳鋼%劉相燕%王洲%劉凡英
진강%류상연%왕주%류범영
CC趋化因子受体7%血管内皮生长因子-C%非小细胞肺癌%淋巴结转移
CC趨化因子受體7%血管內皮生長因子-C%非小細胞肺癌%淋巴結轉移
CC추화인자수체7%혈관내피생장인자-C%비소세포폐암%림파결전이
CC chemokine receptor 7%Vascular endothelial growth factor-C%Non-small cell lung cancer%Lymph node metastasis
目的 观察CC趋化因子受体7(CCR7)及血管内皮生长因子-C(VEGF-C)的表达对非小细胞肺癌(NSCLC)淋巴结转移潜能的影响.方法 临床收集行肺癌完全切除加系统淋巴结清扫的55例NSCLC患者的肺癌组织、淋巴结组织及正常肺组织标本.逆转录-聚合酶链反应(RT-PCR)检测CCR7及VEGF-C mRNA的表达；免疫组织化学检测CCR7及VEGF-C蛋白的表达.应用x2检验比较CCR7及VEGF-C表达差异,并应用Logistic回归分析判定肺癌淋巴结转移的独立相关因素.结果 肺癌组织中有39例(70.9％) CCR7 mRNA及32例(58.2％) VEGF-C mRNA表达阳性,有38例(69.1％) CCR7蛋白及29例(52.7％) VEGF-C蛋白表达阳性,且均与淋巴结转移密切相关(P＜0.05).33例有转移的淋巴结中CCR7、VEGF-C mRNA的阳性表达率分别为84.8％、66.7％,分别明显高于无转移的淋巴结(27.3％、18.2％,P＜0.05).Logistic多因素回归分析结果显示:肺癌组织中CCR7 mRNA的阳性表达[比值比(OR)=10.275,P＜0.01]及VEGF-C mRNA的阳性表达(OR=5.550,P＜0.05)是NSCLC患者淋巴结转移的独立危险因素.结论 CCR7及VEGF-C在NSCLC患者的肺癌组织及淋巴结转移灶中表达均明显增高,并与肺癌淋巴结转移密切相关；CCR7及VEGF-C的表达可能促进了NSCLC淋巴结转移.
目的 觀察CC趨化因子受體7(CCR7)及血管內皮生長因子-C(VEGF-C)的錶達對非小細胞肺癌(NSCLC)淋巴結轉移潛能的影響.方法 臨床收集行肺癌完全切除加繫統淋巴結清掃的55例NSCLC患者的肺癌組織、淋巴結組織及正常肺組織標本.逆轉錄-聚閤酶鏈反應(RT-PCR)檢測CCR7及VEGF-C mRNA的錶達；免疫組織化學檢測CCR7及VEGF-C蛋白的錶達.應用x2檢驗比較CCR7及VEGF-C錶達差異,併應用Logistic迴歸分析判定肺癌淋巴結轉移的獨立相關因素.結果 肺癌組織中有39例(70.9％) CCR7 mRNA及32例(58.2％) VEGF-C mRNA錶達暘性,有38例(69.1％) CCR7蛋白及29例(52.7％) VEGF-C蛋白錶達暘性,且均與淋巴結轉移密切相關(P＜0.05).33例有轉移的淋巴結中CCR7、VEGF-C mRNA的暘性錶達率分彆為84.8％、66.7％,分彆明顯高于無轉移的淋巴結(27.3％、18.2％,P＜0.05).Logistic多因素迴歸分析結果顯示:肺癌組織中CCR7 mRNA的暘性錶達[比值比(OR)=10.275,P＜0.01]及VEGF-C mRNA的暘性錶達(OR=5.550,P＜0.05)是NSCLC患者淋巴結轉移的獨立危險因素.結論 CCR7及VEGF-C在NSCLC患者的肺癌組織及淋巴結轉移竈中錶達均明顯增高,併與肺癌淋巴結轉移密切相關；CCR7及VEGF-C的錶達可能促進瞭NSCLC淋巴結轉移.
목적 관찰CC추화인자수체7(CCR7)급혈관내피생장인자-C(VEGF-C)적표체대비소세포폐암(NSCLC)림파결전이잠능적영향.방법 림상수집행폐암완전절제가계통림파결청소적55례NSCLC환자적폐암조직、림파결조직급정상폐조직표본.역전록-취합매련반응(RT-PCR)검측CCR7급VEGF-C mRNA적표체；면역조직화학검측CCR7급VEGF-C단백적표체.응용x2검험비교CCR7급VEGF-C표체차이,병응용Logistic회귀분석판정폐암림파결전이적독립상관인소.결과 폐암조직중유39례(70.9％) CCR7 mRNA급32례(58.2％) VEGF-C mRNA표체양성,유38례(69.1％) CCR7단백급29례(52.7％) VEGF-C단백표체양성,차균여림파결전이밀절상관(P＜0.05).33례유전이적림파결중CCR7、VEGF-C mRNA적양성표체솔분별위84.8％、66.7％,분별명현고우무전이적림파결(27.3％、18.2％,P＜0.05).Logistic다인소회귀분석결과현시:폐암조직중CCR7 mRNA적양성표체[비치비(OR)=10.275,P＜0.01]급VEGF-C mRNA적양성표체(OR=5.550,P＜0.05)시NSCLC환자림파결전이적독립위험인소.결론 CCR7급VEGF-C재NSCLC환자적폐암조직급림파결전이조중표체균명현증고,병여폐암림파결전이밀절상관；CCR7급VEGF-C적표체가능촉진료NSCLC림파결전이.
Objective To explore the effects of CC chemokine receptor 7 (CCR7) and vascular endothelial growth factor-C (VEGF-C) expression on lymph node metastatic potentiality of non-small cell lung cancer (NSCLC).Methods The samples of cancer tissues,lymph nodes and normal lung tissues from 55 patients with NSCLC who underwent complete resection were examined by using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry to detect CCR7 and VEGF-C expression.The difference in the CCR7 and VEGF-C expression was compared by x2 test.Logistic regression analysis was performed to determine the independent risk factors of lymph node metastasis of NSCLC.Re sults In lung cancer tissues,CCR7 mRNA was expressed in 39 cases (70.9％) and VEGF-C mRNA in 32 cases (58.2％),and CCR7 protein was expressed in 38 cases (69.1％) and VEGF-C protein in 29 cases (52.7％),which was positively correlated to lymph node metastasis (P ＜ 0.05).The expression rate of CCR7 and VEGF-C in metastatic lymph nodes (27.3％ and 18.2％ respectively) was higher than in non-metastatic lymph nodes (P ＜ 0.05).Logistic regression analysis revealed that CCR7 mRNA expression [odds ratio (OR) =10.275,P ＜ 0.01] and VEGF-C mRNA expression in tumors (OR =5.550,P ＜0.05) were independent risk factors of lymph node metastasis of NSCLC.Conclusion CCR7 and VEGF-C were highly expressed in lung cancer and the metastatic lymph nodes,with significant correlation with lymph node metastasis of NSCLC.The expression of CCR7 and VEGF-C promotes lymphatic metastasis of lung cancer and might be a clinical predictor of lymph node metastasis of patients with NSCLC.
