中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
738-740,封3
,共4页
杨欣%胡静雯%张治%邱满堂%徐磊%钱以淳%游庆军%尹荣%许林
楊訢%鬍靜雯%張治%邱滿堂%徐磊%錢以淳%遊慶軍%尹榮%許林
양흔%호정문%장치%구만당%서뢰%전이순%유경군%윤영%허림
肺鳞癌%转移%磷脂酰肌醇蛋白聚糖-5
肺鱗癌%轉移%燐脂酰肌醇蛋白聚糖-5
폐린암%전이%린지선기순단백취당-5
Lung squamous cell carcinoma%Metastasis%Glypican-5
目的 探讨磷脂酰肌醇蛋白聚糖-5(GPC-5)在肺鳞癌中的表达变化及其临床意义.方法 应用实时定量聚合酶链反应(Real-time PCR)的方法检测64例肺鳞癌组织及其对应的肺正常组织中的GPC-5基因的表达.应用免疫组织化学方法验证GPC-5在肺鳞癌组织芯片(包含75例鳞癌组织标本)中的表达.结果 GPC-5 mRNA和蛋白表达水平在肺鳞癌组织中均较正常组织显著下降(P＜0.01)；有淋巴结转移组较无淋巴结转移组GPC-5 mRNA和蛋白表达水平显著降低(29.0074±6.4013比11.4849±2.5066,P＜0.05)；病理分期ⅡA～B组的GPC-5 mRNA表达水平较ⅠA～B组表达显著下降(28.0566±3.8333比11.9384±6.6651,P＜0.05)；Spearman相关分析显示GPC-5 mRNA表达水平与淋巴结转移呈显著正相关(r=0.270,P＜O.05).结论 GPC-5可能是1种新的抑癌基因,且与肺鳞癌的淋巴结转移密切相关.
目的 探討燐脂酰肌醇蛋白聚糖-5(GPC-5)在肺鱗癌中的錶達變化及其臨床意義.方法 應用實時定量聚閤酶鏈反應(Real-time PCR)的方法檢測64例肺鱗癌組織及其對應的肺正常組織中的GPC-5基因的錶達.應用免疫組織化學方法驗證GPC-5在肺鱗癌組織芯片(包含75例鱗癌組織標本)中的錶達.結果 GPC-5 mRNA和蛋白錶達水平在肺鱗癌組織中均較正常組織顯著下降(P＜0.01)；有淋巴結轉移組較無淋巴結轉移組GPC-5 mRNA和蛋白錶達水平顯著降低(29.0074±6.4013比11.4849±2.5066,P＜0.05)；病理分期ⅡA～B組的GPC-5 mRNA錶達水平較ⅠA～B組錶達顯著下降(28.0566±3.8333比11.9384±6.6651,P＜0.05)；Spearman相關分析顯示GPC-5 mRNA錶達水平與淋巴結轉移呈顯著正相關(r=0.270,P＜O.05).結論 GPC-5可能是1種新的抑癌基因,且與肺鱗癌的淋巴結轉移密切相關.
목적 탐토린지선기순단백취당-5(GPC-5)재폐린암중적표체변화급기림상의의.방법 응용실시정량취합매련반응(Real-time PCR)적방법검측64례폐린암조직급기대응적폐정상조직중적GPC-5기인적표체.응용면역조직화학방법험증GPC-5재폐린암조직심편(포함75례린암조직표본)중적표체.결과 GPC-5 mRNA화단백표체수평재폐린암조직중균교정상조직현저하강(P＜0.01)；유림파결전이조교무림파결전이조GPC-5 mRNA화단백표체수평현저강저(29.0074±6.4013비11.4849±2.5066,P＜0.05)；병리분기ⅡA～B조적GPC-5 mRNA표체수평교ⅠA～B조표체현저하강(28.0566±3.8333비11.9384±6.6651,P＜0.05)；Spearman상관분석현시GPC-5 mRNA표체수평여림파결전이정현저정상관(r=0.270,P＜O.05).결론 GPC-5가능시1충신적억암기인,차여폐린암적림파결전이밀절상관.
Objective To investigate glypican-5 (GPC-5) expression in lung squamous cell carcinomas and its clinical significance.Methods GPC-5 was detected in 64 cases of lung squamous cell carcinoma and corresponding normal lung tissue by real-time quantitative polymerase chain reaction (PCR),which was also validated in human lung squamous cell carcinoma tissue microarrays (TMA,containing 75 cases of squamous cell carcinoma tissue and adjacent tissue) by using immunohistochemistry.Results The expression levels of GPC-5 mRNA and protein in squamous cell carcinomas were significantly lower than in matched normal lung tissues (P ＜ 0.001).The expression levels of GPC-5 mRNA and protein in the lymph node metastasis group were lower than those in the non-metastasis group (29.0074 ±6.4013 vs 11.4849 ±2.5066 folder,P ＜0.05).The expression levels of GPC-5 mRNA in the pTNM Ⅱ A-B group were lower than those in the pTNM Ⅰ A-B group (28.0566 ± 3.8333 vs 11.9384 ± 6.6651 folder,P ＜ 0.05).Spearman analysis revealed that the expression levels of GPC-5 mRNA were significantly correlated with lymph node metastasis (r =0.270,P ＜ 0.05).Conclusion GPC-5 may be a new tumor suppressor gene,and is closely related with the lymph node metastasis of lung squamous cell carcinoma.
