中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
741-742
,共2页
肺癌%分子病理学%组织学
肺癌%分子病理學%組織學
폐암%분자병이학%조직학
Lung carcinoma%Molecular pathology%Histology
目的 探讨肺癌在细胞病理学样本组织学分类及分子学检测解决方案.方法 120例肺癌细胞学样本在常规涂片做出定性诊断并部分予以组织学分类后,结合细胞蜡块切片进行甲状腺转录因子-1(TTF-1)、p63、E-钙黏蛋白(E-cad)、细胞角蛋白(CK5/6)、上皮膜抗原(EMA)、突触素(Syn)、神经细胞黏附分子(CD56)、嗜铬素A(CgA)、视网膜钙蛋白(CR)等8项的免疫组织化学染色后再分类并对两者进行比较；部分腺癌病例进行表皮生长因子受体(EGFR)突变检测.结果 常规涂片肺癌组织学总体分型率及非小细胞癌的分型率均显著低于细胞蜡块并结合免疫组织化学染色基础上的分型率(39.2％比88.3％；29.7％比85.1％),两者差异有统计学意义(X2=60.359、72.098,P＜0.01)；EGFR突变检测94.7％ (36/38)成功.结论 肺癌细胞学样本应在常规涂片、细胞蜡块切片并结合免疫组织化学染色再进行组织学分类；细胞蜡块提供了细胞学样本分子病理学检测的有效平台.
目的 探討肺癌在細胞病理學樣本組織學分類及分子學檢測解決方案.方法 120例肺癌細胞學樣本在常規塗片做齣定性診斷併部分予以組織學分類後,結閤細胞蠟塊切片進行甲狀腺轉錄因子-1(TTF-1)、p63、E-鈣黏蛋白(E-cad)、細胞角蛋白(CK5/6)、上皮膜抗原(EMA)、突觸素(Syn)、神經細胞黏附分子(CD56)、嗜鉻素A(CgA)、視網膜鈣蛋白(CR)等8項的免疫組織化學染色後再分類併對兩者進行比較；部分腺癌病例進行錶皮生長因子受體(EGFR)突變檢測.結果 常規塗片肺癌組織學總體分型率及非小細胞癌的分型率均顯著低于細胞蠟塊併結閤免疫組織化學染色基礎上的分型率(39.2％比88.3％；29.7％比85.1％),兩者差異有統計學意義(X2=60.359、72.098,P＜0.01)；EGFR突變檢測94.7％ (36/38)成功.結論 肺癌細胞學樣本應在常規塗片、細胞蠟塊切片併結閤免疫組織化學染色再進行組織學分類；細胞蠟塊提供瞭細胞學樣本分子病理學檢測的有效平檯.
목적 탐토폐암재세포병이학양본조직학분류급분자학검측해결방안.방법 120례폐암세포학양본재상규도편주출정성진단병부분여이조직학분류후,결합세포사괴절편진행갑상선전록인자-1(TTF-1)、p63、E-개점단백(E-cad)、세포각단백(CK5/6)、상피막항원(EMA)、돌촉소(Syn)、신경세포점부분자(CD56)、기락소A(CgA)、시망막개단백(CR)등8항적면역조직화학염색후재분류병대량자진행비교；부분선암병례진행표피생장인자수체(EGFR)돌변검측.결과 상규도편폐암조직학총체분형솔급비소세포암적분형솔균현저저우세포사괴병결합면역조직화학염색기출상적분형솔(39.2％비88.3％；29.7％비85.1％),량자차이유통계학의의(X2=60.359、72.098,P＜0.01)；EGFR돌변검측94.7％ (36/38)성공.결론 폐암세포학양본응재상규도편、세포사괴절편병결합면역조직화학염색재진행조직학분류；세포사괴제공료세포학양본분자병이학검측적유효평태.
Objective To study histological classification and molecular detection solutions of cytopathology samples in lung cancer.Methods 120 cases of lung cancer cytopathology samples have made qualitative diagnosis in conventional smear,and have made the histological classification in part,and then carried out 8 immunohistochemical staining of thyroid transcription factor 1 (TIF-1),p63,E-calcium mucins (E-CAD),creatine kinase 5/6 (CK5/6),epithelial membrane antigen (EMA),synaptic element (Syn),neural cell adhesion molecule 56 (CD56),chromogranin A (CgA) and Calretinin (CR) combined with cell block section,classification again and comparative study both;Part cases of adenocarcinoma have made epidermal growth factor receptor (EGFR) mutation detection.Results Overall parting rate of conventional smear lung cancer histologic and parting rate of non-small cell carcinoma were significantly lower than that in parting rate basis on cell block combined with immunohistochemical stains (39.2％ vs.88.3％ ;29.7％ vs.85.1％,x2 =60.359,72.098,P ＜0.01),EGFR mutation detection 94.7％ (36/38) success.Conclusion Lung cancer cell biology sample should be conducted conventional smear,cell blocksection and combined with immunohistochemical staining,and then make histologic classification;Cell blockprovides a effective platform for cytopathology samples detection.
