CAJ | 학술논문

目的观察丝裂霉素C(MMC)体外预处理供体脾细胞和器官的联合方案延长小鼠心脏移植物存活,探讨其作用机制.方法 MMC体外处理供体脾细胞后输注给受体,并用MMC灌注供体心脏.结果经供体脾细胞输注联合器官预处理,小鼠移植物存活从7.0d延至28.5 d.脾细胞输注或联合方案可使脾脏CD4+ CD25 +/CD4+比例从15.0％降至7.4％、8.6％,CD4+ Foxp3 +/CD4+ 比例从15.5％升至18.2％、18.6％.供体脾细胞经MMC处理后细胞凋亡从15.5％增至23.2％.结论静脉输注MMC处理的供体脾细胞或联合供体器官预处理,可明显延长小鼠心脏移植物存活.MMC可能通过供体细胞凋亡诱导调节性T细胞而发挥作用.
목적 관찰사렬매소C(MMC)체외예처리공체비세포화기관적연합방안연장소서심장이식물존활,탐토기작용궤제.방법 MMC체외처리공체비세포후수주급수체,병용MMC관주공체심장.결과 경공체비세포수주연합기관예처리,소서이식물존활종7.0d연지28.5 d.비세포수주혹연합방안가사비장CD4+ CD25 +/CD4+비례종15.0％강지7.4％、8.6％,CD4+ Foxp3 +/CD4+ 비례종15.5％승지18.2％、18.6％.공체비세포경MMC처리후세포조망종15.5％증지23.2％.결론 정맥수주MMC처리적공체비세포혹연합공체기관예처리,가명현연장소서심장이식물존활.MMC가능통과공체세포조망유도조절성T세포이발휘작용.
Objective To investigate the effect of pretreatment of donor splenocytes and grafts with mitomycin C (MMC) on heart allograft survival,and to demonstrate the mechanism of function.Methods Donor splenocytes from donor mice were incubated with MMC in vitro and were then transfused into recipient mice.The heart allograft was perfused with MMC prior to harvest.Results Donor splenocyte transfusion (DST) combined with MMC-graft pretreatment prolonged heart allograft survival from 7.0 to 28.5 days.DST or combination strategy significantly decreased CD4 + CD25 + population in CD4 + T cells from 15.0％ to 7.4％ and 8.6％,and meanwhile increased CD4 + Foxp3 + T cells from 15.5％ to 18.2％ and 18.6％.MMC incubation in vitro induced apoptosis of donor splenocytes from 15.5％ to 23.2％.Conclusion Transfusion of MMC-treated donor splenocytes either alone or combined with graft pretreatment with MMC could significantly prolong heart allograft survival in mice.MMC pretreatment could induce apoptosis of donor cells,increase production of regulatory T cells,and thus achieve donor-specific immunosuppression.