CAJ | 학술논문

目的测定缺血再灌注损伤模型肾组织中肾损伤分子-1(KIM-1)表达,探讨其在反映肾脏热缺血损伤方面的应用价值.方法建立肾缺血再灌注损伤模型,根据热缺血时间长短随机将大鼠分为对照组(热缺血0 min组)、热缺血15 min组、热缺血30 min组、热缺血45 min组和热缺血60 min组.再灌注8h后,苏木素-伊红(HE)染色观察肾脏病理变化,检测血清肌酐(Cr)值,免疫组织化学法检测肾组织KIM-1表达、Western blot法检测胞质中KIM-1的含量.结果随着热缺血时间的延长,肾小管肿胀、坏死程度加重,血清Cr值增高,由(32.1±5.7) μmol/L升高至(96.8±9.5) μmol/L,肾组织KIM-1表达增强,累积吸光度(IA)值由372±62升高至40 928±238.45 min热缺血时间内,肾组织KIM-1表达与Cr值呈正相关(r=0.91,P＜0.05).结论大鼠肾组织KIM-1随热缺血时间延长而表达增强,可联合病理学检查,从形态学和功能学两方面反映热缺血损伤对肾脏功能的影响程度.
목적 측정결혈재관주손상모형신조직중신손상분자-1(KIM-1)표체,탐토기재반영신장열결혈손상방면적응용개치.방법 건립신결혈재관주손상모형,근거열결혈시간장단수궤장대서분위대조조(열결혈0 min조)、열결혈15 min조、열결혈30 min조、열결혈45 min조화열결혈60 min조.재관주8h후,소목소-이홍(HE)염색관찰신장병리변화,검측혈청기항(Cr)치,면역조직화학법검측신조직KIM-1표체、Western blot법검측포질중KIM-1적함량.결과 수착열결혈시간적연장,신소관종창、배사정도가중,혈청Cr치증고,유(32.1±5.7) μmol/L승고지(96.8±9.5) μmol/L,신조직KIM-1표체증강,루적흡광도(IA)치유372±62승고지40 928±238.45 min열결혈시간내,신조직KIM-1표체여Cr치정정상관(r=0.91,P＜0.05).결론 대서신조직KIM-1수열결혈시간연장이표체증강,가연합병이학검사,종형태학화공능학량방면반영열결혈손상대신장공능적영향정도.
Objective To verify weather kidney injury molecular-1 (KIM-1 could reflect the function of rat kidney undergoing warm ischemia injury (WII).Methods An ischemia reperfusion model was established.Based on the length of warm ischemia time,rats were divided into control group (no WII),15-min WII group,30-min WII group,45-min WII group and 60-min WII group.After reperfusion for 8 h,allograft samples were collected and cut into sections for hematoxylin and eosin (HE) staining,immunohistochemistry and Western blotting,respectively.Blood was collected for further tests.Results With the extension of the warm ischemia time,the kidney tubules became swollen and necrotic.Cr went from (32.1 ±5.7) μmol/L to (96.8 ±9.5) μmol/L,but cumulative absorbance value (IA) of KIM-1 went from 372 ± 62 to 40 928 ± 238.The KIM-1 expression was correlated with Cr (r =0.91,P ＜ 0.01).Conclusion As a biomarker,the expression of KIM-1 get stronger and stranger with the the extension of warm ischemia time.In combination with pathological examination,KIM-1 could reflect function of the kidney under WII.