中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
772-774,封3
,共4页
於文丽%李晓宁%吴小燕%王行环%刘同族%丁国华
於文麗%李曉寧%吳小燕%王行環%劉同族%丁國華
어문려%리효저%오소연%왕행배%류동족%정국화
白细胞介素-17%糖尿病,肾小管-间质%α-平滑肌肌动蛋白%核因子-κB
白細胞介素-17%糖尿病,腎小管-間質%α-平滑肌肌動蛋白%覈因子-κB
백세포개소-17%당뇨병,신소관-간질%α-평활기기동단백%핵인자-κB
Interleukin-17%Diabetes,renal tubular-interstitial%α-smooth muscle actin%Nuclear factor-kappa B
目的 观察白细胞介素-17 (IL-17)在糖尿病大鼠肾组织的表达,探讨其参与肾小管-间质病变的机制.方法 55只雄性SD大鼠,随机选择35只以50 mg/kg链脲佐菌素(STZ)制备糖尿病大鼠模型,剩余20只为正常对照组.于2、4周末采用酶联免疫吸附试验(ELISA)方法检测血、尿中IL-17.4周末采用免疫组织化学染色及Western blot检测肾组织中IL-17、α-平滑肌肌动蛋白(α-SMA)及核因子-κB p65(NF-κB p65).结果 糖尿病组大鼠2周末血、尿中IL-17水平分别为(11.51 ±5.62)、(79.21±14.53)ng/L,与对照组比较差异有统计学意义[(8.87±3.35)、(6.75±1.13) ng/L,P＜0.01],4周末升高更明显；IL-17在正常对照组肾组织无表达,仅在糖尿病大鼠肾小管和肾间质中高表达.糖尿病大鼠肾组织中IL-17表达与尿中IL-17(r =0.715,P＜0.05)及肾组织中α-SMA(r =0.708,P＜0.05)、NF-κBp65(r=0.638,P＜0.01)表达均呈正相关.结论 IL-17参与糖尿病肾病早期肾小管-间质病变的可能机制为促进α-SMA表达增加.
目的 觀察白細胞介素-17 (IL-17)在糖尿病大鼠腎組織的錶達,探討其參與腎小管-間質病變的機製.方法 55隻雄性SD大鼠,隨機選擇35隻以50 mg/kg鏈脲佐菌素(STZ)製備糖尿病大鼠模型,剩餘20隻為正常對照組.于2、4週末採用酶聯免疫吸附試驗(ELISA)方法檢測血、尿中IL-17.4週末採用免疫組織化學染色及Western blot檢測腎組織中IL-17、α-平滑肌肌動蛋白(α-SMA)及覈因子-κB p65(NF-κB p65).結果 糖尿病組大鼠2週末血、尿中IL-17水平分彆為(11.51 ±5.62)、(79.21±14.53)ng/L,與對照組比較差異有統計學意義[(8.87±3.35)、(6.75±1.13) ng/L,P＜0.01],4週末升高更明顯；IL-17在正常對照組腎組織無錶達,僅在糖尿病大鼠腎小管和腎間質中高錶達.糖尿病大鼠腎組織中IL-17錶達與尿中IL-17(r =0.715,P＜0.05)及腎組織中α-SMA(r =0.708,P＜0.05)、NF-κBp65(r=0.638,P＜0.01)錶達均呈正相關.結論 IL-17參與糖尿病腎病早期腎小管-間質病變的可能機製為促進α-SMA錶達增加.
목적 관찰백세포개소-17 (IL-17)재당뇨병대서신조직적표체,탐토기삼여신소관-간질병변적궤제.방법 55지웅성SD대서,수궤선택35지이50 mg/kg련뇨좌균소(STZ)제비당뇨병대서모형,잉여20지위정상대조조.우2、4주말채용매련면역흡부시험(ELISA)방법검측혈、뇨중IL-17.4주말채용면역조직화학염색급Western blot검측신조직중IL-17、α-평활기기동단백(α-SMA)급핵인자-κB p65(NF-κB p65).결과 당뇨병조대서2주말혈、뇨중IL-17수평분별위(11.51 ±5.62)、(79.21±14.53)ng/L,여대조조비교차이유통계학의의[(8.87±3.35)、(6.75±1.13) ng/L,P＜0.01],4주말승고경명현；IL-17재정상대조조신조직무표체,부재당뇨병대서신소관화신간질중고표체.당뇨병대서신조직중IL-17표체여뇨중IL-17(r =0.715,P＜0.05)급신조직중α-SMA(r =0.708,P＜0.05)、NF-κBp65(r=0.638,P＜0.01)표체균정정상관.결론 IL-17삼여당뇨병신병조기신소관-간질병변적가능궤제위촉진α-SMA표체증가.
Objective To investigate the association between the expression of interleukin-17 (IL-17) in renal tubuleinterstitium and diabetic nephropathy.Methods 55 rats were randomly divided into three groups:control group,diabetic rat group.Diabetic rat group was injected 50 mg/kg streptozoticin (STZ).In the 2th,4th week,serum and urine IL-17 were measured with enzyme-linked immunosorbent assay (ELISA).The expression of IL-17,α-smooth muscle actin (α-SMA) and nuclear factor-κB p65 (NF-κB p65) in the kidney were assessed by immunohistochemisty staining and Western blotting.Results Serum and urine IL-17 in diabetic rats were [(11.51 ±5.62),(79.21 ± 14.53) ng/L],which were significantly upreguated as compared with those in control group rats (P ＜ 0.01),which were [(8.87 ± 3.35),(6.75 ± 1.13) ng/L].The expression of IL-17 was only observed in renal tubuleinterstitium of diabetic rats,the expression of IL-17 in renal tubuleinterstitium of diabetic rats was positively correlated with urine IL-17 (r =0.715,P ＜ 0.05),and the expression of α-SMA (r =0.708,P ＜ 0.05)、NF-κB p65 (r =0.638,P ＜ 0.01) in renal.Conclusion The effect of IL-17 on pathogenesis and progression of diabetic nephropathy in diabetic rat might be up-regulating the expression of α-SMA.
