中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
778-780
,共3页
前列腺癌%微小RNA-26b%细胞迁移%细胞侵袭
前列腺癌%微小RNA-26b%細胞遷移%細胞侵襲
전렬선암%미소RNA-26b%세포천이%세포침습
Prostate cancer%microRNA-26b%Cell migration%Cell invasion
目的 观察上调微小RNA(miRNA,miR)-26b表达对前列腺癌细胞生物学行为的影响.方法 实时荧光定量聚合酶链反应(FQ-PCR)检测miR-26b在5种前列腺癌细胞株和良性前列腺组织中的表达.将miR-26b模拟物和阴性对照miRNA分别转染至miR-26b表达最低的LNCaP细胞.噻唑蓝(MTT)法检测miR-26b表达对LNCaP细胞的增殖能力,流式细胞仪检测其细胞凋亡率,细胞划痕实验和Transwell小室侵袭实验分别检测其细胞迁移和侵袭能力.结果 与良性前列腺组织比较,miR-26b在5种前列腺癌细胞株中均呈低表达,以LNCaP细胞株(0.214±0.019)最为明显.同阴性对照组比较,转染miR-26b的LNCaP细胞miR-26b表达(65.597±11.426)增强310倍,其迁移细胞数[(29±3)个]和细胞穿膜数[(30±2)个]均较对照组的(158±16)个和(147±15)个显著减少,差异有统计学意义(P＜0.05),而其细胞增殖能力、细胞凋亡率和细胞周期则变化不明显.结论 miR-26b在前列腺癌细胞株低表达,上调前列腺癌细胞miR-26b的表达能够抑制前列腺癌细胞的迁移和侵袭能力.
目的 觀察上調微小RNA(miRNA,miR)-26b錶達對前列腺癌細胞生物學行為的影響.方法 實時熒光定量聚閤酶鏈反應(FQ-PCR)檢測miR-26b在5種前列腺癌細胞株和良性前列腺組織中的錶達.將miR-26b模擬物和陰性對照miRNA分彆轉染至miR-26b錶達最低的LNCaP細胞.噻唑藍(MTT)法檢測miR-26b錶達對LNCaP細胞的增殖能力,流式細胞儀檢測其細胞凋亡率,細胞劃痕實驗和Transwell小室侵襲實驗分彆檢測其細胞遷移和侵襲能力.結果 與良性前列腺組織比較,miR-26b在5種前列腺癌細胞株中均呈低錶達,以LNCaP細胞株(0.214±0.019)最為明顯.同陰性對照組比較,轉染miR-26b的LNCaP細胞miR-26b錶達(65.597±11.426)增彊310倍,其遷移細胞數[(29±3)箇]和細胞穿膜數[(30±2)箇]均較對照組的(158±16)箇和(147±15)箇顯著減少,差異有統計學意義(P＜0.05),而其細胞增殖能力、細胞凋亡率和細胞週期則變化不明顯.結論 miR-26b在前列腺癌細胞株低錶達,上調前列腺癌細胞miR-26b的錶達能夠抑製前列腺癌細胞的遷移和侵襲能力.
목적 관찰상조미소RNA(miRNA,miR)-26b표체대전렬선암세포생물학행위적영향.방법 실시형광정량취합매련반응(FQ-PCR)검측miR-26b재5충전렬선암세포주화량성전렬선조직중적표체.장miR-26b모의물화음성대조miRNA분별전염지miR-26b표체최저적LNCaP세포.새서람(MTT)법검측miR-26b표체대LNCaP세포적증식능력,류식세포의검측기세포조망솔,세포화흔실험화Transwell소실침습실험분별검측기세포천이화침습능력.결과 여량성전렬선조직비교,miR-26b재5충전렬선암세포주중균정저표체,이LNCaP세포주(0.214±0.019)최위명현.동음성대조조비교,전염miR-26b적LNCaP세포miR-26b표체(65.597±11.426)증강310배,기천이세포수[(29±3)개]화세포천막수[(30±2)개]균교대조조적(158±16)개화(147±15)개현저감소,차이유통계학의의(P＜0.05),이기세포증식능력、세포조망솔화세포주기칙변화불명현.결론 miR-26b재전렬선암세포주저표체,상조전렬선암세포miR-26b적표체능구억제전렬선암세포적천이화침습능력.
Objective To investigate the effects of the up-regulated expression of microRNA (miRNA,miR)-26b on the biological properties of prostate cancer cells.Methods The expression of miR-26b in PC23,PC-3M,DU-145,LNCaP,22RV1 cell lines and benign prostate tissue were detected by Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR).miR-26b mimics and control miRNA were transfected into LNCaP cells by Lipofectamine 2000.The cellular growth activity was assayed by methyl thiazol tetrazolium (MTT) assay,the apoptosis and cell cycles was tested by flow cytometry.Wound healing assay and Transwell assay were applied to measure cell migration and invasion.Results The expression of miR-26b in prostate cancer cell lines was lower than that in benign prostate tissue.Compared to the control,the expression of miR-26b was increased by 310 folds in LNCaP transfected with miR 26b,which numbers of migrated and penetrated reduce significantly from (158 ± 16) and (147 ± 15) to (29 ±3) and (30 ±2) respectively (P ＜0.05),whereas,cellular growth activity,apoptosis and cell cycles were not changed significantly.Conclusion The expression of miR-26b is down-regulation in prostate cancer cell lines.Up-regulation of miR-26b inhibits LNCaP cell' s migration and invasion.
