中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
799-801
,共3页
杨玉宝%刘省臣%宋扬%高忠礼%李林%阚金庆
楊玉寶%劉省臣%宋颺%高忠禮%李林%闞金慶
양옥보%류성신%송양%고충례%리림%감금경
酒精缺血坏死性股骨头%基因芯片
酒精缺血壞死性股骨頭%基因芯片
주정결혈배사성고골두%기인심편
Alcohol avascular necrosis of femoral head%Gene chip
目的 观察微小RNA(miRNA)在酒精缺血坏死性与正常股骨头骨组织的表达变化,探讨miRNA基因在两者中的变化关系.方法 分别对5例酒精性股骨头与5例正常股骨头骨组织进行miRNA基因芯片检测,可见有上调基因has-miR-29a-st和下调基因has-miR-486-5p-st,分别对上调和下调基因行逆转录-聚合酶链反应(RT-PCR).结果 miRNA基因芯片共检测酒精缺血坏死性与正常股骨头骨组织400条基因,对明显上调has-miR-29a-st和下调has-miR-486-5p-st基因片段行RT-PCR检测,正常股骨头和酒精坏死性股骨头上调基因的2-△△Ct平均值为1.06±0.09和1.93±1.12,下调基因的2-△△Ct平均值为1.88±25.37和1.70 ±0.98(变化＞2倍且P＜0.05).结论 酒精缺血坏死性与正常股骨头骨组织存在明显miRNA变化,其可能在股骨头坏死发生过程中可能起着重要的调控作用.
目的 觀察微小RNA(miRNA)在酒精缺血壞死性與正常股骨頭骨組織的錶達變化,探討miRNA基因在兩者中的變化關繫.方法 分彆對5例酒精性股骨頭與5例正常股骨頭骨組織進行miRNA基因芯片檢測,可見有上調基因has-miR-29a-st和下調基因has-miR-486-5p-st,分彆對上調和下調基因行逆轉錄-聚閤酶鏈反應(RT-PCR).結果 miRNA基因芯片共檢測酒精缺血壞死性與正常股骨頭骨組織400條基因,對明顯上調has-miR-29a-st和下調has-miR-486-5p-st基因片段行RT-PCR檢測,正常股骨頭和酒精壞死性股骨頭上調基因的2-△△Ct平均值為1.06±0.09和1.93±1.12,下調基因的2-△△Ct平均值為1.88±25.37和1.70 ±0.98(變化＞2倍且P＜0.05).結論 酒精缺血壞死性與正常股骨頭骨組織存在明顯miRNA變化,其可能在股骨頭壞死髮生過程中可能起著重要的調控作用.
목적 관찰미소RNA(miRNA)재주정결혈배사성여정상고골두골조직적표체변화,탐토miRNA기인재량자중적변화관계.방법 분별대5례주정성고골두여5례정상고골두골조직진행miRNA기인심편검측,가견유상조기인has-miR-29a-st화하조기인has-miR-486-5p-st,분별대상조화하조기인행역전록-취합매련반응(RT-PCR).결과 miRNA기인심편공검측주정결혈배사성여정상고골두골조직400조기인,대명현상조has-miR-29a-st화하조has-miR-486-5p-st기인편단행RT-PCR검측,정상고골두화주정배사성고골두상조기인적2-△△Ct평균치위1.06±0.09화1.93±1.12,하조기인적2-△△Ct평균치위1.88±25.37화1.70 ±0.98(변화＞2배차P＜0.05).결론 주정결혈배사성여정상고골두골조직존재명현miRNA변화,기가능재고골두배사발생과정중가능기착중요적조공작용.
Objective To observe expression variation of microRNA (miRNA) between bone tissue of alcohol avascular necrosis femoral head and normal femoral head and discuss miRNA change relation between them.Methods It is tested for miRNA for five normal femoral head tissue and alcohol avascular necrosis femoral head tissue.it is thus clear that there are up regulation has-miR-29a-st and downregulation genes has-miR-486-5p-st.they are went on reverse transcriptase-polymerase chain reaction (RT-PCR).Results Four hundred genes were analysed between between bone tissue of alcohol avascular necrosis femoral head and normal femoral head with miRNA gene chip.clear down regulation has-miR-486-5p-st and up regulation has-miR-29a-st were went on PCR,the average value of has-miR-29a-st 2-~Ct is 1.06 ±0.09 and 1.93 ± 1.12 for bone tissue of alcohol avascular necrosis femoral head and normal femoral head; the average value of has-miR-486-5p-st2-~Ct is 1.88 ± 25.37 and 1.70 ± 0.98 (variation ＞ 2 times moreover P ＜ 0.05).Conclusion There is obvious up regulation and down regulation miRNA genes between bone tissue of normal femoral head and alcohol avascular necrosis femoral head.which is possible to take on main regulation action to speeding up necrosis femoral head.
