CAJ | 학술논문

目的探讨新型强化型抗菌磷酸钙骨水泥(CPC)的机械及抗菌性能.方法使用经过季铵盐基团改性的壳聚糖丝强化CPC骨水泥,通过涂板技术及激光共聚焦显微镜观察其抗菌效果；通过噻唑蓝(MTT)比色法及4’,6-二脒基-2-苯基吲哚(DAPI)染色观察其生物相容性；通过三点弯曲试验检测其力学性能.结果通过激光共聚焦可见新材料具有较好的抗菌效果,其细菌菌落比例为(0.10±0.09)％；三点弯曲试验表明经过壳聚糖丝改进后的机械性能[(17.3±4.5) MPa与(16.9±3.9)MPa]较之普通骨水泥有明显改善[(5.3±1.4)MPa]；并且这样的改进对材料的生物相容性没有影响,其毒性试验显示新材料相容性比值为(97.5±3.3)％,细胞增殖实验3天吸光度值分别为0.237±0.025、0.503 ±0.015、0.726±0.032.结论新型的季铵盐壳聚糖强化型骨水泥无细胞毒性,具有良好的机械性能及抗菌效果.
목적 탐토신형강화형항균린산개골수니(CPC)적궤계급항균성능.방법 사용경과계안염기단개성적각취당사강화CPC골수니,통과도판기술급격광공취초현미경관찰기항균효과；통과새서람(MTT)비색법급4’,6-이미기-2-분기신타(DAPI)염색관찰기생물상용성；통과삼점만곡시험검측기역학성능.결과 통과격광공취초가견신재료구유교호적항균효과,기세균균락비례위(0.10±0.09)％；삼점만곡시험표명경과각취당사개진후적궤계성능[(17.3±4.5) MPa여(16.9±3.9)MPa]교지보통골수니유명현개선[(5.3±1.4)MPa]；병차저양적개진대재료적생물상용성몰유영향,기독성시험현시신재료상용성비치위(97.5±3.3)％,세포증식실험3천흡광도치분별위0.237±0.025、0.503 ±0.015、0.726±0.032.결론 신형적계안염각취당강화형골수니무세포독성,구유량호적궤계성능급항균효과.
Objective To evaluation the mechanical behavior and the ability of antibacterial of the new calcium phosphate bone cement (CPC).Methods The CPC was reinforced by chitosan fiber with hydroxypropyltrimethyl ammonium chloride chitosan (HACC).The spread plate analysis and confocal laser scanning microscopy were used for bacterial adherence assay.The strength of the samples was investigated by a standard three-point bending test.Cell toxicity and proliferation of cells were also invested.Results The new bone cement showed a good antibacterial activity which had the least amount of surviving bacterial as (0.10 ± 0.09) ％.The results of three-point bending test also showed the incorporation of chitosan fiber [(17.3 ± 4.5) MPa and (16.9 ± 3.9) MPa] significantly increased the mechanical strength of CPC [(5.3 ± 1.4) MPa].And the reinforcement has no harmful to the biocompatibility of CPC.Extracts of new bone cement showed non-cytotoxic (97.5 ± 3.3)％.The methyl thiazol tetrazolium (MTT) activity of cells is 0.237 ± 0.025,0.503 ± 0.015,0.726 ± 0.032 during 3 days cultured.Conclusion CPC combined with chitosan fiber with HACC can meet the needs of strength,biocompatibility and antibacterial activity.