CAJ | 학술논문

目的观察5-氟尿嘧啶(5-Fu)和顺铂(CP)联合使用对肿瘤细胞是否具有协同抗增殖的作用.方法利用蛋白组织学分析,包括双向凝胶电泳及基质辅助激光解吸/离子化-飞行时间质谱来探索5-Fu或/和CP治疗后与抗增殖相关的蛋白.结果 5-Fu处理后有25种蛋白上调节和16种蛋白下调,CP处理后有20种蛋白上调和11种蛋白下调.5-Fu和CP联合使用的最佳浓度为5-Fu:0.5 μmol/L和CP:1.0 μmol/L,两者联合使用72 h后出现协同效应,其协同效应是通过调节膜凋亡受体通路和线粒体凋亡通路而实现的,同时联合使用2种药物后宫颈癌细胞凋亡的敏感性提高.结论 5-Fu和CP联合使用可通过协同诱导细胞凋亡而抑制宫颈癌细胞的增殖.
목적 관찰5-불뇨밀정(5-Fu)화순박(CP)연합사용대종류세포시부구유협동항증식적작용.방법 이용단백조직학분석,포괄쌍향응효전영급기질보조격광해흡/리자화-비행시간질보래탐색5-Fu혹/화CP치료후여항증식상관적단백.결과 5-Fu처리후유25충단백상조절화16충단백하조,CP처리후유20충단백상조화11충단백하조.5-Fu화CP연합사용적최가농도위5-Fu:0.5 μmol/L화CP:1.0 μmol/L,량자연합사용72 h후출현협동효응,기협동효응시통과조절막조망수체통로화선립체조망통로이실현적,동시연합사용2충약물후궁경암세포조망적민감성제고.결론 5-Fu화CP연합사용가통과협동유도세포조망이억제궁경암세포적증식.
Objective To investigate the synergistic effect of 5-fluorouracil (5-Fu) and cisplatin on cervical carcinoma cells in vitro.Methods Using proteomics analyses,including two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDITOF-MS),we investigated the antiproliferative effect-related proteins after treatment with 5-Fu and/or CP.Results It was found 25 proteins were up-regulated and 16 down-regulated after 5-Fu treatment as determined by spot volume,and 20 proteins were up-regulated and 11 down-regulated after CP treatment as determined by spot volume.The optimal concentrations for combined use of 5-Fu and CP were as follows:5-Fu:0.5 μ mol/L,and CP:1.0 μmol/L,and the synergistic effects occurred after 72 h.The combination of 5-Fu and CP engaged two apoptotic pathways including the membrane death receptor-mediated apoptosis pathway and the mitochondrial apoptotic pathway.Moreover,the combination of 5-Fu and CP resulted in a remarkably increasing susceptibility to apoptosis.Conluusion The combination of 5-Fu and CP suppresses the growth of cervical carcinoma cells by synergistic effect with the induction of apoptosis.In vitro the synergistic effect of 5-Fu and CP may provide the theoretical guidance for clinical treatment of cervical cancer patients.