CAJ | 학술논문

目的检测人肝细胞癌(HCC)患者分泌型的磷脂酶A2-ⅡA(PLA2G2A) mRNA表达,探讨PLA2G2A与HCC发生发展的关系.方法采用SYBR Green I荧光实时定量聚合酶链反应(FQ-PCR)方法分别检测51例HCC组织和配对的癌旁肝组织,以及9例正常肝组织中PLA2G2AmRNA表达,以β-肌动蛋白(β-actin)为内参,根据相对定量公式(2-△△CT)计算PLA2G2A mRNA相对表达量,并分析HCC组织中PLA2G2A mRNA表达水平与临床病理特征之间的关系.结果肝癌组织中PLA2G2A mRNA表达量明显低于配对的癌旁肝组织和正常肝组织,仅是癌旁肝组织的0.2446倍(P＜0.01),是正常肝组织的0.2339倍(P＜0.01)；PLA2G2A mRNA的表达水平和HCC组织的分化程度呈正相关(P＜0.05),与HCC患者血清的AFP蛋白的表达量呈负相关(P＜0.05)；有肝硬化背景的患者HCC组织中PLA2G2A mRNA表达量明显高于无肝硬化背景的患者(P＜0.05).结论 PLA2G2A mRNA的表达缺失可能与肝细胞癌的发生和进展有关.
목적 검측인간세포암(HCC)환자분비형적린지매A2-ⅡA(PLA2G2A) mRNA표체,탐토PLA2G2A여HCC발생발전적관계.방법 채용SYBR Green I형광실시정량취합매련반응(FQ-PCR)방법분별검측51례HCC조직화배대적암방간조직,이급9례정상간조직중PLA2G2AmRNA표체,이β-기동단백(β-actin)위내삼,근거상대정량공식(2-△△CT)계산PLA2G2A mRNA상대표체량,병분석HCC조직중PLA2G2A mRNA표체수평여림상병리특정지간적관계.결과 간암조직중PLA2G2A mRNA표체량명현저우배대적암방간조직화정상간조직,부시암방간조직적0.2446배(P＜0.01),시정상간조직적0.2339배(P＜0.01)；PLA2G2A mRNA적표체수평화HCC조직적분화정도정정상관(P＜0.05),여HCC환자혈청적AFP단백적표체량정부상관(P＜0.05)；유간경화배경적환자HCC조직중PLA2G2A mRNA표체량명현고우무간경화배경적환자(P＜0.05).결론 PLA2G2A mRNA적표체결실가능여간세포암적발생화진전유관.
Objective To investigate the correlations between hepatocellular carcinoma (HCC) and phospholipase A2 group ⅡA (PLA2G2A) mRNA expression level.Methods SYBR Green I real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect PLA2G2A mRNA expression in 51 HCC tissues and their paired noncancerous liver tissues and 9 normal liver tissues.β-actin gene was used as an internal control and the relative expression level of PLA2G2A mRNA was analyzed using the method of 2-△△CT.The correlation between PLA2G2A mRNA levels in HCC tissues and clinicopathological features was analyzed.Results PLA2G2A mRNA levels in HCC tissues was 0.2446 times lower than that in their paired noncancerous liver tissues (P ＜ 0.01) and 0.2339 times lower than that in normal liver tissues (P ＜ 0.01).PLA2G2A mRNA was positively correlated with tumor differentiation (P ＜ 0.05) and negatively with AFP level (P ＜ 0.05) of HCC.PLA2G2A mRNA levels in HCC tissues underlying liver cirrhosis was higher than those without liver cirrhosis (P ＜ 0.05).Conclusion The downregulation of PLA2G2A may get involved in the tumorgenesis and may be help for diagnosis of HCC.