中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
834-835
,共2页
梁卫明%陈义雄%戴维%蔡良真
樑衛明%陳義雄%戴維%蔡良真
량위명%진의웅%대유%채량진
Dickkopf-1%癌,肝细胞%介入治疗%手术
Dickkopf-1%癌,肝細胞%介入治療%手術
Dickkopf-1%암,간세포%개입치료%수술
Dickkopf-1%Carcinoma,hepatocellular%Interventional therapy%Operation
目的 观察原发性肝癌(PLC)患者手术前后、介入治疗前后血清Dickkopf-1(DKK1)表达水平的变化.方法 酶联免疫吸附试验法(ELISA)检测30例正常人以及60例PLC患者血清DKK1水平,并比较20例Ⅰ期PLC患者手术前后以及18例Ⅱ期PLC患者介入治疗前后血清DKK1水平.结果 血清DKK1水平与PLC患者临床分期明显相关、与PLC患者性别、年龄无明显相关(P＞0.05)；PLC患者血清DKK1水平Ⅲ期(11.54±0.80) μg/L明显高于Ⅱ期(9.16±0.73) μg/L、Ⅱ期(9.16±0.73) μg/L明显高于Ⅰ期(6.24±1.12) μg/L、Ⅰ期(6.24 ±1.12) μg/L明显高于正常对照组(3.14 ±0.84) μg/L、两者比较差异有统计学意义(P＜0.01)；手术后PLC患者血清DKK1水平(5.58 ±1.09) μg/L明显低于手术前(6.24±1.12) μg/L、介入治疗后PLC患者血清DKK1水平(8.81 ±0.80) μg/L明显低于介入治疗前(9.16±0.79)μg/L,两者比较差异有统计学意义(P＜0.01).结论 DKK1可能在PLC发展过程中发挥一定的作用.
目的 觀察原髮性肝癌(PLC)患者手術前後、介入治療前後血清Dickkopf-1(DKK1)錶達水平的變化.方法 酶聯免疫吸附試驗法(ELISA)檢測30例正常人以及60例PLC患者血清DKK1水平,併比較20例Ⅰ期PLC患者手術前後以及18例Ⅱ期PLC患者介入治療前後血清DKK1水平.結果 血清DKK1水平與PLC患者臨床分期明顯相關、與PLC患者性彆、年齡無明顯相關(P＞0.05)；PLC患者血清DKK1水平Ⅲ期(11.54±0.80) μg/L明顯高于Ⅱ期(9.16±0.73) μg/L、Ⅱ期(9.16±0.73) μg/L明顯高于Ⅰ期(6.24±1.12) μg/L、Ⅰ期(6.24 ±1.12) μg/L明顯高于正常對照組(3.14 ±0.84) μg/L、兩者比較差異有統計學意義(P＜0.01)；手術後PLC患者血清DKK1水平(5.58 ±1.09) μg/L明顯低于手術前(6.24±1.12) μg/L、介入治療後PLC患者血清DKK1水平(8.81 ±0.80) μg/L明顯低于介入治療前(9.16±0.79)μg/L,兩者比較差異有統計學意義(P＜0.01).結論 DKK1可能在PLC髮展過程中髮揮一定的作用.
목적 관찰원발성간암(PLC)환자수술전후、개입치료전후혈청Dickkopf-1(DKK1)표체수평적변화.방법 매련면역흡부시험법(ELISA)검측30례정상인이급60례PLC환자혈청DKK1수평,병비교20례Ⅰ기PLC환자수술전후이급18례Ⅱ기PLC환자개입치료전후혈청DKK1수평.결과 혈청DKK1수평여PLC환자림상분기명현상관、여PLC환자성별、년령무명현상관(P＞0.05)；PLC환자혈청DKK1수평Ⅲ기(11.54±0.80) μg/L명현고우Ⅱ기(9.16±0.73) μg/L、Ⅱ기(9.16±0.73) μg/L명현고우Ⅰ기(6.24±1.12) μg/L、Ⅰ기(6.24 ±1.12) μg/L명현고우정상대조조(3.14 ±0.84) μg/L、량자비교차이유통계학의의(P＜0.01)；수술후PLC환자혈청DKK1수평(5.58 ±1.09) μg/L명현저우수술전(6.24±1.12) μg/L、개입치료후PLC환자혈청DKK1수평(8.81 ±0.80) μg/L명현저우개입치료전(9.16±0.79)μg/L,량자비교차이유통계학의의(P＜0.01).결론 DKK1가능재PLC발전과정중발휘일정적작용.
Objective To investigate the changes of serum Dickkopf-1 (DKK1) in patients with primary liver carcinoma (PLC) before and after operation and interventional therapy.Methods Enzyme linked immunosorbent assay (ELISA) was used to detect the serum DKK1 levels in 60 cases of PLC and 30 cases of normal control subjects.The serum DKK1 levels in 20 cases of stage Ⅰ PLC pre-and post-operation,and 18 cases of stage Ⅱ PLC pre-and post-interventional therapy were compared.Results Serum DKK1 was correlated with clinical stage of patients with PLC,but not with sex and age.The serum DKK1 levels in patients with stage Ⅲ of PLC (11.54 ± 0.80) μg/L were higher than in those with stage Ⅱ (9.16±0.73) μg/L,(P＜0.01),those in stage Ⅱ (9.16±0.73) μg/L higher than in stage Ⅰ (6.24 ± 1.12) μg/L,(P ＜ 0.01),and those in stage Ⅰ (6.24 ± 1.12) μg/L higher than in normal controls (3.14 ± 0.84) μg/L.(P ＜ 0.05).The serum DKK1 levels were remarkably reduced in patients with PLC after operation or interventional therapy (P ＜ 0.01).Conclusion These results suggest that the level of DKK1 might play a significant role in the progression of PLC.
