中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
4期
836-838
,共3页
成德雷%徐浩%华荣%徐新建%仇焕%杜洪涛%张庆桥
成德雷%徐浩%華榮%徐新建%仇煥%杜洪濤%張慶橋
성덕뢰%서호%화영%서신건%구환%두홍도%장경교
布加综合征%淤血性肝硬化%缺氧诱导因子-1α%核因子-κB%肿瘤坏死因子-α
佈加綜閤徵%淤血性肝硬化%缺氧誘導因子-1α%覈因子-κB%腫瘤壞死因子-α
포가종합정%어혈성간경화%결양유도인자-1α%핵인자-κB%종류배사인자-α
Budd-Chiari syndrome%Stasis cirrhosis%Hypoxia inducible factor-1α%Nuclear factor-kappaB%Tumor necrosis factor-α
目的 观察缺氧在布加综合征(BCS)导致淤血性肝硬化形成过程中的作用.方法 选取2012年5月至2012年7月在徐州医学院附属医院收治的30例BCS致淤血性肝硬化患者作为实验组,选取10名健康志愿者作为对照组.采用酶联免疫法检测受试对象血清中缺氧诱导因子-1α(HIF-1α)、核因子-κB(NF-κB)及肿瘤坏死因子-α(TNF-α)的含量.采用独立样本t检验统计分析3个指标组间差异性.结果 实验组血清中HIF-1α、NF-κB及TNF-α的含量分别为:(2.49±0.70) μg/L、(307.37±140.65) ng/L及(48.16±8.57) ng/L；对照组血清中3个指标的含量分别为:(1.72 ±0.10) μg/L、(211.16±6.05) ng/L及(38.97±3.84)ng/L.实验组各个指标含量均高于对照组,组间差异有统计学意义(P＜0.05).结论 缺氧以及HIF-1α、NF-κB 2种缺氧敏感性转录因子的活化与BCS导致淤血性肝硬化形成有关；早期对BCS患者进行血管再通治疗,可能会减慢(甚至逆转)缺氧导致淤血性肝硬化进程.
目的 觀察缺氧在佈加綜閤徵(BCS)導緻淤血性肝硬化形成過程中的作用.方法 選取2012年5月至2012年7月在徐州醫學院附屬醫院收治的30例BCS緻淤血性肝硬化患者作為實驗組,選取10名健康誌願者作為對照組.採用酶聯免疫法檢測受試對象血清中缺氧誘導因子-1α(HIF-1α)、覈因子-κB(NF-κB)及腫瘤壞死因子-α(TNF-α)的含量.採用獨立樣本t檢驗統計分析3箇指標組間差異性.結果 實驗組血清中HIF-1α、NF-κB及TNF-α的含量分彆為:(2.49±0.70) μg/L、(307.37±140.65) ng/L及(48.16±8.57) ng/L；對照組血清中3箇指標的含量分彆為:(1.72 ±0.10) μg/L、(211.16±6.05) ng/L及(38.97±3.84)ng/L.實驗組各箇指標含量均高于對照組,組間差異有統計學意義(P＜0.05).結論 缺氧以及HIF-1α、NF-κB 2種缺氧敏感性轉錄因子的活化與BCS導緻淤血性肝硬化形成有關；早期對BCS患者進行血管再通治療,可能會減慢(甚至逆轉)缺氧導緻淤血性肝硬化進程.
목적 관찰결양재포가종합정(BCS)도치어혈성간경화형성과정중적작용.방법 선취2012년5월지2012년7월재서주의학원부속의원수치적30례BCS치어혈성간경화환자작위실험조,선취10명건강지원자작위대조조.채용매련면역법검측수시대상혈청중결양유도인자-1α(HIF-1α)、핵인자-κB(NF-κB)급종류배사인자-α(TNF-α)적함량.채용독립양본t검험통계분석3개지표조간차이성.결과 실험조혈청중HIF-1α、NF-κB급TNF-α적함량분별위:(2.49±0.70) μg/L、(307.37±140.65) ng/L급(48.16±8.57) ng/L；대조조혈청중3개지표적함량분별위:(1.72 ±0.10) μg/L、(211.16±6.05) ng/L급(38.97±3.84)ng/L.실험조각개지표함량균고우대조조,조간차이유통계학의의(P＜0.05).결론 결양이급HIF-1α、NF-κB 2충결양민감성전록인자적활화여BCS도치어혈성간경화형성유관；조기대BCS환자진행혈관재통치료,가능회감만(심지역전)결양도치어혈성간경화진정.
Objective To investigate whether hypoxia plays an important role in the formation of stasis cirrhosis caused by Budd-Chiari syndrome (BCS).Methods 30 consecutive BCS patients who were diagnosed with cirrhosis through Magnetic resonance imaging and Doppler ultrasound were selected as the experimental group between May 2012 and July 2012.Over the same period 10 healthy volunteers were selected as control group from the Medical Examination Center of our Hospital,all of them were found no cirrhosis by Doppler ultrasound.And then the serum concentrations of hypoxia inducible factor-1α (HIF-1α),nuclear factor-kappaB (NF-κB) and tumor necrosis factor-α (TNF-α) were defined by enzyme-linked-immunosorbent-assay.The results were analyzed with independent sample t-test.Results In the experimental group,the serum concentrations of HIF-1 α,NF-κB and TNF-α [(2.49 ± 0.70) μg/L,(307.37 ± 140.65) ng/L and (48.16 ± 8.57) ng/L] were higher than those in control group [(1.72 ± 0.10) μg/L,(211.16 ± 6.05) ng/L and (38.97 ± 3.84) ng/L,t =5.87,2.14,3.26,P ＜ 0.05 respectively].Conclusion Hypoxia and the activation of NF-κB and HIF-1α were related to the formation of stasis cirrhosis caused by BCS; if conducted recanalization therapy on BCS patients in early phase,the process of stasis cirrhosis caused by hypoxia could slow down or even reverse.
