CAJ | 학술논문

目的观察汉族与维吾尔族肾癌患者之间血清蛋白表达谱的变化,建立维吾尔族、汉族不同民族肾癌血清诊断模型并筛查2个不同民族肾癌患者之间的差异蛋白.方法采用表面增强激光解吸离子化飞行时间质谱技术和弱阳离子交换蛋白芯片(CM10)检测血清标本,对新疆地区24例维吾尔族肾癌患者与24例维吾尔族健康对照血清蛋白表达谱进行检测分析；对36例汉族肾癌患者与36例汉族健康对照血清蛋白质指纹图谱进行检测分析.结果维吾尔族肾癌组与维吾尔族健康对照组组间得到差异有统计学意义的6个蛋白质峰,分别为5935、5945、5911、13 766、13 965、6887,建立的诊断模型的敏感度为100.00％(24/24),特异度为91.67％ (22/24)；汉族肾癌组与汉族健康对照组组间得到的差异有统计学意义的4个蛋白质峰分别为5937、5345、5947、5912,建立的诊断模型的敏感度为91.67％(33/36),特异度为94.44％(34/36)；维吾尔族与汉族两个不同民族肾癌之间的血清蛋白质指纹图谱差异有统计学意义(P＜0.05).质荷比为6887、13 766、13 965的3个蛋白峰为维吾尔族肾癌诊断模型所独有,而质荷比为5345的蛋白峰为汉族肾癌诊断模型所独有.结论新疆地区维吾尔族肾癌与汉族肾癌患者之间血清蛋白质指纹图谱差异有统计学意义,质荷比为6887、13 766、13 965、5345的蛋白峰可能是维汉不同民族肾癌的差异蛋白.其中M/Z为6887、13 766、13 965蛋白峰对应的可能蛋白质分别为为角蛋白、甲状腺素转运蛋白及干扰素诱导的跨膜蛋白-1.
목적 관찰한족여유오이족신암환자지간혈청단백표체보적변화,건립유오이족、한족불동민족신암혈청진단모형병사사2개불동민족신암환자지간적차이단백.방법 채용표면증강격광해흡리자화비행시간질보기술화약양리자교환단백심편(CM10)검측혈청표본,대신강지구24례유오이족신암환자여24례유오이족건강대조혈청단백표체보진행검측분석；대36례한족신암환자여36례한족건강대조혈청단백질지문도보진행검측분석.결과 유오이족신암조여유오이족건강대조조조간득도차이유통계학의의적6개단백질봉,분별위5935、5945、5911、13 766、13 965、6887,건립적진단모형적민감도위100.00％(24/24),특이도위91.67％ (22/24)；한족신암조여한족건강대조조조간득도적차이유통계학의의적4개단백질봉분별위5937、5345、5947、5912,건립적진단모형적민감도위91.67％(33/36),특이도위94.44％(34/36)；유오이족여한족량개불동민족신암지간적혈청단백질지문도보차이유통계학의의(P＜0.05).질하비위6887、13 766、13 965적3개단백봉위유오이족신암진단모형소독유,이질하비위5345적단백봉위한족신암진단모형소독유.결론 신강지구유오이족신암여한족신암환자지간혈청단백질지문도보차이유통계학의의,질하비위6887、13 766、13 965、5345적단백봉가능시유한불동민족신암적차이단백.기중M/Z위6887、13 766、13 965단백봉대응적가능단백질분별위위각단백、갑상선소전운단백급간우소유도적과막단백-1.
Objective To observe serum protein change between Uygur with renal cell cancer (RCC) and Han with RCC and to construct different ethnic(including Han and Uygur) diagnostic model of RCC in XinJiang and screen serum differentially expressed protein between Han with renal cancer and Uygur with renal cancer in Xinjiang;Methods To use weak cation exchange and hydrophobic surface protein chip (CM10) and Surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) to detect the serum protein.To detected serum specimens from patients (36 Han and 24 Uygur)with RCC and 60 healthy controls so as to construct different ethnic RCC diagnostic model and screen out disparate serum proteins between Han with RCC and Uygur with RCC.Results Obvious disparity existed between healthy controls and RCC patients in Uygur.Diagnostic model was composed and established by six proteins (5935,5945,5911,13 766,13 965,6887).The specificity and sensitivity were 91.67％ (22/24) and 100.00％ (24/24) ; Obvious disparity existed between healthy controls and RCC patients in Han.Total were four proteins peaks (5937,5345,5947,5912),and diagnostic model composed and established.The specificity and sensitivity were 94.44％ (34/36)and 91.67％ (33/36) ;disparity existed between Uygur RCC patients and Han RCC patients in terms of serum protein finger prints.Three proteins peaks M/Z 6887,13 766,13 965 existed diagnostic model of Uygur patients group while lacked in Han patients group,meanwhile,M/Z 5345 existed diagnostic model of Han patients group while lacked in Uygur patients group.Conclusion It has difference for protein finger prints between Han with RCC and Uygur with RCC in Xinjing.M/Z 6887,13 766,13 965,5345 may be differential markers between Uygur with RCC and Han with RCC and M/Z 6887,13 766,13 965 may be Keratin,transthyretin and interferon-inducedtransmembrane protein-1.