CAJ | 학술논문

目的观察Ras同源类似物C(RhoC) mRNA和蛋白在皮肤恶性黑色素瘤组织中的表达,探讨与临床病理特征的关系.方法应用逆转录聚合酶链反应和Western blot检测32例皮肤恶性黑色素瘤组织中RhoC mRNA和蛋白的表达,以皮肤交界痣和正常皮肤组织作为对照.结果在皮肤恶性黑色素瘤组织中,RhoC mRNA和蛋白的相对含量分别为0.468±0.230及0.769±0.411,较交界痣及正常皮肤明显升高(P＜0.05),在临床Ⅰ、Ⅱ、Ⅲ和Ⅳ期,RhoC mRNA和蛋白的相对含量分别为0.196±0.042、0.411±0.049、0.765±0.091和0.422±0.050、0.763±0.093、1.215±0.076,随临床分期及淋巴结和远处转移RhoC mRNA和蛋白的相对含量逐级升高(P＜0.05),但与年龄、性别无明显相关(P＞0.05).结论肿瘤转移相关因子RhoC在皮肤恶性黑色素瘤组织中高表达,且与临床分期及淋巴结和远处转移有关,RhoC可能参与皮肤恶性黑色素瘤的发生发展,且与其侵袭转移过程密切相关.
목적 관찰Ras동원유사물C(RhoC) mRNA화단백재피부악성흑색소류조직중적표체,탐토여림상병리특정적관계.방법 응용역전록취합매련반응화Western blot검측32례피부악성흑색소류조직중RhoC mRNA화단백적표체,이피부교계지화정상피부조직작위대조.결과 재피부악성흑색소류조직중,RhoC mRNA화단백적상대함량분별위0.468±0.230급0.769±0.411,교교계지급정상피부명현승고(P＜0.05),재림상Ⅰ、Ⅱ、Ⅲ화Ⅳ기,RhoC mRNA화단백적상대함량분별위0.196±0.042、0.411±0.049、0.765±0.091화0.422±0.050、0.763±0.093、1.215±0.076,수림상분기급림파결화원처전이RhoC mRNA화단백적상대함량축급승고(P＜0.05),단여년령、성별무명현상관(P＞0.05).결론 종류전이상관인자RhoC재피부악성흑색소류조직중고표체,차여림상분기급림파결화원처전이유관,RhoC가능삼여피부악성흑색소류적발생발전,차여기침습전이과정밀절상관.
Objective To investigate the expression of Ras homology C (RhoC) mRNA and protein in cutaneous malignant melanoma and its relationship with clinical pathological features of cutaneous malignant melanoma.Methods The expression of RhoC mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting in 32 cases of cutaneous malignant melanoma,as well as cutaneous junctional nevus and normal skin tissue were selected as controls.Results The expressions of RhoC mRNA and protein in cutaneous malignant melanoma were 0.468 ± 0.230 and 0.769 ± 0.411,which were higher than those in cutaneous junctional nevus and normal skin tissue (P ＜0.05).Furthermore,The expressions of RhoC mRNA and protein were 0.196 ± 0.042,0.411 ± 0.049,0.765 ±0.091 and 0.422 ±0.050,0.763 ±0.093,1.215 ±0.076 in the stage Ⅰ,Ⅱ,Ⅲand Ⅳof cutaneous malignant melanoma.The level of RhoC was correlated with increasing of clinical stage or the presence of lymphatic and distant metastases,however it was not correlated with age and gender.Conclusion Overexpression of RhoC,which as a molecular switch to control tumor metastasis,was associated with the carcinogenesis and progression of cutaneous malignant melanoma,and promoted invasion and metastasis in cutaneous malignant melanoma.