CAJ | 학술논문

目的建立显微镜下小切口-单椎板切除后钳夹型脊髓损伤(SCI)模型.方法将30只成年SD大鼠随机分为实验组(n=18)和对照组(n=12),两组均在显微镜下行小切口-胸11椎板咬除,实验组用动脉瘤夹夹闭脊髓2 min,对照组不夹闭脊髓.术后进行组织学染色、磁共振(MRI)扫描及BBB评分.结果实验组大鼠术后出现不同程度尿储留和肠功能下降,组织学染色见脊髓结构不规则、片状出血、神经元坏死及髓鞘空泡化.MRI提示出血和水肿并存；对照组未见明显异常；对照组术后BBB评分均为21分,实验组术后1d评分均为0分,1周后为(4.58 ±0.67)分,2周后恢复至(13.00±1.04)分,各时间点均低于对照组(P＜0.01).结论显微镜下行小切口-单椎板切除术建立钳夹型脊髓损伤模型具有良好的稳定性和可重复性.
목적 건립현미경하소절구-단추판절제후겸협형척수손상(SCI)모형.방법 장30지성년SD대서수궤분위실험조(n=18)화대조조(n=12),량조균재현미경하행소절구-흉11추판교제,실험조용동맥류협협폐척수2 min,대조조불협폐척수.술후진행조직학염색、자공진(MRI)소묘급BBB평분.결과 실험조대서술후출현불동정도뇨저류화장공능하강,조직학염색견척수결구불규칙、편상출혈、신경원배사급수초공포화.MRI제시출혈화수종병존；대조조미견명현이상；대조조술후BBB평분균위21분,실험조술후1d평분균위0분,1주후위(4.58 ±0.67)분,2주후회복지(13.00±1.04)분,각시간점균저우대조조(P＜0.01).결론 현미경하행소절구-단추판절제술건립겸협형척수손상모형구유량호적은정성화가중복성.
Objective The establish the clip compressive spinal cord injury model through small incision and mono-laminectomy under microscope.Methods Thirty Sprague-Dawley (SD) rats were randomly divided into the injury group (n =18) and the control group (n =12).Small incision and monolaminectomy at T11 were performed to the injury group,followed by 2-min compressive injury induced by the aneurysm clip.The same procedure was done to the control group except spinal cord injury.Histological staining was performed one day post-operation,as well as MRI scanning and BBB Locomotor Rating Scale.Results Urinary retention and declination of intestinal function could be witnessed in the injury group,along with hemorrhage,edema,necrosis of neurons and myelin vacuolization histologically.MRI showed mixed signals intramedullarily.None of these were witnessed in the control group.BBB scores in the control group remained at 21 throughout the whole experiment,while those in the injury group possessed 0 immediately after the operation,4.58 ±0.67 and 13.00 ± 1.04 at 1st and 2nd week post-operation respectively (P ＜ 0.01).Conclusion Meticulous operation,small non-related injury and disturbance to the spinal cord are the merits of small incision and mono-laminectomy under microscope.