CAJ | 학술논문

目的观察Kruppel样因子4(KLF4)在创伤新生血管中的表达及在体外对内皮细胞功能的影响.方法用24只大鼠作皮肤创伤模型,分别于创伤后即刻、2、4、6、8、10、12、14 d处死并取创缘组织作免疫组织化学染色；用KLF4小干扰RNA(siRNA)转染人脐静脉内皮细胞(HUVECs)48 h后,检测其增殖、迁移和成管能力变化.结果 KLF4在新生血管内皮细胞中有表达；转染KLF4 siRNA后,HUVECs的吸光度(A)值、迁移距离和成管环数明显降低,分别为0.948±0.043、(206.0±5.6)μm、(12.0±0.7)个(P＜0.05).结论 KLF4对皮肤创伤新生血管的形成起重要作用.
목적 관찰Kruppel양인자4(KLF4)재창상신생혈관중적표체급재체외대내피세포공능적영향.방법 용24지대서작피부창상모형,분별우창상후즉각、2、4、6、8、10、12、14 d처사병취창연조직작면역조직화학염색；용KLF4소간우RNA(siRNA)전염인제정맥내피세포(HUVECs)48 h후,검측기증식、천이화성관능력변화.결과 KLF4재신생혈관내피세포중유표체；전염KLF4 siRNA후,HUVECs적흡광도(A)치、천이거리화성관배수명현강저,분별위0.948±0.043、(206.0±5.6)μm、(12.0±0.7)개(P＜0.05).결론 KLF4대피부창상신생혈관적형성기중요작용.
Objective To investigate the expression of Kruppel-like factor4 (KLF4) in neovascular endothelial cells during the process of wound healing and the effect on endothelial cells function in vitro.Methods The skin wound model was established in 24 rats,and the marginal tissues were taken for immnunohistochemical staining after the rats were killed immediately,2,4,6,8,10,12,and 14 days after the trauma.Proliferation,migration and tube formation of human unbilical vein endothelial cells (HUVECs) were measured after transfection with KLF4 small interfering RNA (siRNA) for 48 h.Results KLF4 was expressed in neovascular endothelial cells.The absorbance of HUVECs proliferation,the migration distance and tube number were 0.948 ±0.043,(206.0 ±5.6) μm and 12.0 ±0.7 respectively (P ＜0.05) after transfection of KLF4 siRNA.Conclusion KLF4 plays an important role in the vascular formation (angiogenesis) during cutaneous wound healing.