CAJ | 학술논문

目的通过对蚕丝丝素-聚乳酸(PLA)-聚羟基乙酸(PGA)纤维混合编织绳状支架的力学性能分析及生物学评价,探讨使用该支架构建组织工程韧带的可行性.方法将蚕丝-PLA-PGA纤维混合编织,捻拧成绳状支架,蚕丝丝素-PLA-PGA的质量比约为1∶1∶1,每个支架共含单丝7200根.使用电子万能试验机对支架材料进行力学性能测试,并进行如下生物学评价:细胞毒性实验、急性全身毒性试验、皮内刺激试验、热原试验、肌肉埋植实验,并进行分析、评价.结果蚕丝-PLA-PGA纤维混合绳状支架韧性强,为螺旋上升的绳索状,直径2.7mm,力学性能优良.支架无细胞毒性(细胞毒性分级0～1级),无急性全身毒性作用,无皮内刺激反应,不具热原反应,组织相容性良好.结论蚕丝-PLA-PGA编织绳状支架具有优良的力学性能及良好的生物相容性.
목적 통과대잠사사소-취유산(PLA)-취간기을산(PGA)섬유혼합편직승상지가적역학성능분석급생물학평개,탐토사용해지가구건조직공정인대적가행성.방법 장잠사-PLA-PGA섬유혼합편직,념녕성승상지가,잠사사소-PLA-PGA적질량비약위1∶1∶1,매개지가공함단사7200근.사용전자만능시험궤대지가재료진행역학성능측시,병진행여하생물학평개:세포독성실험、급성전신독성시험、피내자격시험、열원시험、기육매식실험,병진행분석、평개.결과 잠사-PLA-PGA섬유혼합승상지가인성강,위라선상승적승색상,직경2.7mm,역학성능우량.지가무세포독성(세포독성분급0～1급),무급성전신독성작용,무피내자격반응,불구열원반응,조직상용성량호.결론 잠사-PLA-PGA편직승상지가구유우량적역학성능급량호적생물상용성.
Objective To evaluate mechanical property and biological property of a rope-shaped scaffold braided by silk-poly (L-lactic acid) (PLA)-polyglycolic acid (PGA) fiber,and investigate the feasibility of fabricating tissue-engineered ligament by using such scaffold.Methods Firstly,a hybrid rope-shaped scaffold containing monofilament 7200 was prepared by twisting a mixture of silkworm silk fibroin,PLA,and PGA fiber filament with a mass ratio of 1∶1∶1.The biomechanical property of the abovementioned silk-PLA-PGA scaffold was measured by using the electric universal testing machine.Secondly,the biological property of the silk-PLA-PGA braided scaffold materials was assessed according to the relevant experiments,including cvtotoxicitv test,acute systematic toxicity test,intracutaneous irritation test,pyrogen test,and muscle implant test.The experimental results were analvzed and assessed by criterion.Results The appearance of the scaffold braided by silk-PLA-PGA was a strong obdurability with a diamter of 2.7 mm.The scaffold had excellent mechanical property,good histocompatibility,no cytotoxicity,no acute systematic toxicity,no skin stimulation,and no pyrogen reaction.Conclusion The scaffold has good biomechanical property and biocompatibility.