CAJ | 학술논문

目的从U251细胞株中分离胶质瘤干细胞并探讨胶质瘤干细胞的抗辐射性能.方法应用无血清培养基悬浮培养法从U251细胞株中分离鉴定胶质瘤干细胞,X线辐射处理72 h后,噻唑蓝(MTT)比色法检测细胞活性,流式细胞仪及免疫荧光染色法检测辐射前后CD133和三磷酸腺苷结合盒转运体成员2 (ABCG2)的表达.结果 U251细胞株中经过无血清培养可以得到悬浮生长的胶质瘤细胞球,具有较强的自我更新和增殖能力；在细胞球中,有3.17％的细胞表达CD133,1.17％表达ABCG2；9 Gy辐射处理72 h后,CD133表达阳性率上升到13.24％,ABCG2表达阳性率上升到8.76％；辐射处理后,细胞活性随辐射剂量增大而显著下降(P＜0.05).结论悬浮培养法得到的肿瘤细胞球有小部分是胶质瘤干细胞；X线辐射处理后,细胞活性随辐射剂量增大而显著下降；胶质瘤干细胞表现出更强的抗辐射损伤性能.
목적 종U251세포주중분리효질류간세포병탐토효질류간세포적항복사성능.방법 응용무혈청배양기현부배양법종U251세포주중분리감정효질류간세포,X선복사처리72 h후,새서람(MTT)비색법검측세포활성,류식세포의급면역형광염색법검측복사전후CD133화삼린산선감결합합전운체성원2 (ABCG2)적표체.결과 U251세포주중경과무혈청배양가이득도현부생장적효질류세포구,구유교강적자아경신화증식능력；재세포구중,유3.17％적세포표체CD133,1.17％표체ABCG2；9 Gy복사처리72 h후,CD133표체양성솔상승도13.24％,ABCG2표체양성솔상승도8.76％；복사처리후,세포활성수복사제량증대이현저하강(P＜0.05).결론 현부배양법득도적종류세포구유소부분시효질류간세포；X선복사처리후,세포활성수복사제량증대이현저하강；효질류간세포표현출경강적항복사손상성능.
Objective To explore the radioresistence of glioma stem cell iso-lated from U251 cell line.Methods U251 cells were cultured in serum-free DMEM/F12 medium,There were forming U251 brain tumor spheres,In order to observe the characteristic of the cell of tumor spheres,the cell of tumor spheres were identifyed by immunohistochemistry and were irradiated by X-rays with different doses,The activ-ity of cell were measured by methyl thiazol tetrazolium (MTT),the marker CD133 and ABCG2 were measured by Flow cytometer.Results Some U251 glioma cells have the capacity to self-renew and generate free-floating brain tumor spheres; CD133 + cells were 3.17％ and ABCG2 ± cells were 1.17％ in tumur sphere,After 9Gy irradiated,CD133 + cells increased to13.24％ and ABC-G2 + cells were 8.76％ ; After ir-radiated,the activity of cells decreased obviously.Conclusion Glioma stem cell can achieve bv cultured in serum-free medium and Glioma stem cell show more radioresistance than glioma cell.