CAJ | 학술논문

目的观察囊状动脉瘤形成过程中蛋白质表达的变化.方法应用双向荧光差异凝胶电泳技术和质谱技术分析兔实验性囊状动脉瘤形成过程的蛋白质组学变化,比较不同形成阶段(0、3、7、14、28 d)囊状动脉瘤组织蛋白质表达谱的差异.结果 113个蛋白点在各实验组间蛋白含量差异有统计学意义(两组间蛋白含量比值≥1.5,P＜0.01)；经质谱分析,15个蛋白质被确定.其中转铁蛋白、乳酸脱氢酶、肌酸激酶、甘油醛-3-磷酸脱氢酶(GAPDH)、钙通道蛋白TRPC1A和碳酸酐酶6个蛋白质功能涉及细胞代谢；α-平滑肌肌动蛋白(α-SMA)、γ非肌动蛋白、SM22和微管蛋白等5个蛋白质与细胞骨架与平滑肌细胞凋亡有关；谷胱甘肽S转移酶、维生素D3结合蛋白、膜联蛋白-1和纤维蛋白原与氧化应激与炎性反应有关.结论应用蛋白质组技术分析实验性囊状动脉瘤形成过程中蛋白质组变化,表明细胞损害和炎症等机制可能参与了囊状动脉瘤的形成.
목적 관찰낭상동맥류형성과정중단백질표체적변화.방법 응용쌍향형광차이응효전영기술화질보기술분석토실험성낭상동맥류형성과정적단백질조학변화,비교불동형성계단(0、3、7、14、28 d)낭상동맥류조직단백질표체보적차이.결과 113개단백점재각실험조간단백함량차이유통계학의의(량조간단백함량비치≥1.5,P＜0.01)；경질보분석,15개단백질피학정.기중전철단백、유산탈경매、기산격매、감유철-3-린산탈경매(GAPDH)、개통도단백TRPC1A화탄산항매6개단백질공능섭급세포대사；α-평활기기동단백(α-SMA)、γ비기동단백、SM22화미관단백등5개단백질여세포골가여평활기세포조망유관；곡광감태S전이매、유생소D3결합단백、막련단백-1화섬유단백원여양화응격여염성반응유관.결론 응용단백질조기술분석실험성낭상동맥류형성과정중단백질조변화,표명세포손해화염증등궤제가능삼여료낭상동맥류적형성.
Objective To identify the proteins involved in the formation of caccular aneurysm.Methods The differentially expressed proteins in rabbit different stage experimental saccular aneurysms were studied by using two-dimensional difference gel electrophoresis.Results A total of 113 spots were found to be significantly different in rabbit different stage experimental saccular aneurysms (the ratio of protein expression in different stage aneurysms ≥ 1.5,P ＜ 0.01).Fifteen proteins were identified by Mass spectrometer among these spots,including 6 metabolic enzymes,5 cell skeleton or cell apoptotic proteins and 4 oxidative stress and inflammation related proteins.Conclusion Proteome alterations in experimental saccular aneurysm tissues were profiled firstly,and celluar injury and inflammation mav be involved in the process of saccular aneurysm formation.