中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
5期
986-988
,共3页
董永强%梁江水%殷桂林%朱水波%张晓明%纪涛
董永彊%樑江水%慇桂林%硃水波%張曉明%紀濤
동영강%량강수%은계림%주수파%장효명%기도
组织因子途径抑制物-2%甲基化%非小细胞肺癌
組織因子途徑抑製物-2%甲基化%非小細胞肺癌
조직인자도경억제물-2%갑기화%비소세포폐암
Tissue factor pathway inhibitor 2%Methylation%Non-small cell lung cancer
目的 检测非小细胞肺癌(NSCLC)组织子途径抑制物-2(TFPI-2)基胞嘧啶-磷酸-鸟嘌呤基序(CpG)岛甲基化的状态,探讨甲基化状态与非小细胞肺癌发生发展及浸润转移的关系.方法 应用甲基化特异性聚合酶链反应(MS-PCR)检测60例非小细胞肺癌组织及相应癌旁组织和10例正常肺组织的TFPI-2基因甲基化状态.结果 非小细胞肺癌组织中TFPI-2基因的甲基化阳性率为38.3％ (23/60),明显高于癌旁组织的6.7％ (4/60)(P＜0.01).10例正常肺组织中均未发生甲基化.临床分期为Ⅰ、Ⅱ、Ⅲ期的患者中TFPI-2基因甲基化阳性率分别为18.2％、53.8％、66.7％ (P＜0.01),肿瘤直径＞5 cm和≤5 cm的患者中TFPI-2基六因甲基化阳性率分别为80.0％、17.5％(P＜0.01),无淋巴结转移和有淋巴结转移的患者中TFPI-2基因甲基化阳性率分别为25.6％、61.9％(P＜0.01).结论 TFPI-2基因在非小细胞肺癌组织中出现异常甲基化,且与临床分期、肿瘤大小及淋巴结是否转移密切相关.
目的 檢測非小細胞肺癌(NSCLC)組織子途徑抑製物-2(TFPI-2)基胞嘧啶-燐痠-鳥嘌呤基序(CpG)島甲基化的狀態,探討甲基化狀態與非小細胞肺癌髮生髮展及浸潤轉移的關繫.方法 應用甲基化特異性聚閤酶鏈反應(MS-PCR)檢測60例非小細胞肺癌組織及相應癌徬組織和10例正常肺組織的TFPI-2基因甲基化狀態.結果 非小細胞肺癌組織中TFPI-2基因的甲基化暘性率為38.3％ (23/60),明顯高于癌徬組織的6.7％ (4/60)(P＜0.01).10例正常肺組織中均未髮生甲基化.臨床分期為Ⅰ、Ⅱ、Ⅲ期的患者中TFPI-2基因甲基化暘性率分彆為18.2％、53.8％、66.7％ (P＜0.01),腫瘤直徑＞5 cm和≤5 cm的患者中TFPI-2基六因甲基化暘性率分彆為80.0％、17.5％(P＜0.01),無淋巴結轉移和有淋巴結轉移的患者中TFPI-2基因甲基化暘性率分彆為25.6％、61.9％(P＜0.01).結論 TFPI-2基因在非小細胞肺癌組織中齣現異常甲基化,且與臨床分期、腫瘤大小及淋巴結是否轉移密切相關.
목적 검측비소세포폐암(NSCLC)조직자도경억제물-2(TFPI-2)기포밀정-린산-조표령기서(CpG)도갑기화적상태,탐토갑기화상태여비소세포폐암발생발전급침윤전이적관계.방법 응용갑기화특이성취합매련반응(MS-PCR)검측60례비소세포폐암조직급상응암방조직화10례정상폐조직적TFPI-2기인갑기화상태.결과 비소세포폐암조직중TFPI-2기인적갑기화양성솔위38.3％ (23/60),명현고우암방조직적6.7％ (4/60)(P＜0.01).10례정상폐조직중균미발생갑기화.림상분기위Ⅰ、Ⅱ、Ⅲ기적환자중TFPI-2기인갑기화양성솔분별위18.2％、53.8％、66.7％ (P＜0.01),종류직경＞5 cm화≤5 cm적환자중TFPI-2기륙인갑기화양성솔분별위80.0％、17.5％(P＜0.01),무림파결전이화유림파결전이적환자중TFPI-2기인갑기화양성솔분별위25.6％、61.9％(P＜0.01).결론 TFPI-2기인재비소세포폐암조직중출현이상갑기화,차여림상분기、종류대소급림파결시부전이밀절상관.
Objective To investigate the methvlation of Cytosine-phosphoric acid-guanine motif (CpG) island of tissue factor pathway inhibitor 2 (TFPI-2) gene in non-small cell long cancer (NSCLC)and the relationship between the aberrant methylation of CpG islaud of TFPI-2 gene and the occurrence,progression,invasion and metastasis of NSCLC.Methods Methylation specific PCR was used to detect the promoter methylation of TFPI-2 gene in cancer tissues from 60 patients with NSCLC and normal lung tissues from 10 patients with pulmonary bullae.Results Promoter methylation of TFPI-2 gene was found in 38.3％ (23/60) of NSCLC tissuees and 6.7％ (4/60) of carcinoma adjacent tissues respectively (P ＜0.01).Aberrant methylation was not found in 10 normal lung tissues.TFPI-2 gene methylation positive rate was 18.2％,53.8％ and 66.7％ in the patients with NSCLC of clinical stage Ⅰ,Ⅱ,Ⅲ (P＜0.01),that was 80.0％ and 17.5％ in tumor diameter ＞ 5 cmand ≤ 5 cm (P＜0.01),and that was 25.6％ and 61.9％ in patients without lymph node metastasis and lymph node metastasis (P ＜ 0.01).Conclusion Aberrant methvlation of TFPI-2 gene was found in NSCLC,which may be associated with the clinical stage,tumor size,and lymph node metastasis.
