CAJ | 학술논문

目的观察聚集素(Clusterin)在特发性弱精子症发生机制中的作用.方法采用非连续密度梯度离心法分离纯化22例特发性弱精子症和24例正常男性精子,通过实时荧光定量聚合酶链反应(FQ-PCR)和Western blot方法,从基因和蛋白水平分别检测Clnsterin的表达差异.结果 FQ-PCR结果表明,Clusterin mRNA在特发性弱精子症患者精子中的表达较正常男性显著降低(0.11 ±0.07比0.98±0.26,t=9.15,P ＜0.001)；Western blot结果示,Clusterin蛋白在特发性弱精子症患者精子中的表达较正常男性亦显著降低(0.66±0.21比1.44 ±0.38,t =6.29,P＜0.05).结论特发性弱精子症患者精子中Clusterin表达量显著降低,可能是导致弱精子症发生的重要环节之一.
목적 관찰취집소(Clusterin)재특발성약정자증발생궤제중적작용.방법 채용비련속밀도제도리심법분리순화22례특발성약정자증화24례정상남성정자,통과실시형광정량취합매련반응(FQ-PCR)화Western blot방법,종기인화단백수평분별검측Clnsterin적표체차이.결과 FQ-PCR결과표명,Clusterin mRNA재특발성약정자증환자정자중적표체교정상남성현저강저(0.11 ±0.07비0.98±0.26,t=9.15,P ＜0.001)；Western blot결과시,Clusterin단백재특발성약정자증환자정자중적표체교정상남성역현저강저(0.66±0.21비1.44 ±0.38,t =6.29,P＜0.05).결론 특발성약정자증환자정자중Clusterin표체량현저강저,가능시도치약정자증발생적중요배절지일.
Objective To investigate the role of clusterin in the pathogenesis of idiopathic asthenozoospermia.Methods We separated and purified the ejaculated spermatozoa from 22 idiopathic asthenozoospermia patients and 24 normozoospermic men by Percoll discontinuous density gradients,and detected the distribution and the expressions of clusterin mRNA and protein by using real-time fluorescent quantitatire polymerase chain reaction (FQ-PCR) and Western blotting separately.Results FQ-PCR revealed that the expression of clusterin mRNA was significantly lower in the spermatozoa of patients with idiopathic asthenozoospermia than in those of the normozoospermic men (0.11 ± 0.07 vs 0.98 ± 0.26,t =9.15,P ＜0.001),and Western blotting confirmed the results of FQ-PCR (0.66 ± 0.21 vs 1.44 ± 0.38,t =6.29,P ＜0.05).Conclusion The expression of clusterin is significantly decreased in the ejaculated spermatozoa of patients with idiopathic asthenozoospermia,which might be one of the causes of idiopathic asthenozoospermia.