中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
5期
1013-1015
,共3页
潘建锋%郭常安%沈剑锋%刘嘉%阎作勤
潘建鋒%郭常安%瀋劍鋒%劉嘉%閻作勤
반건봉%곽상안%침검봉%류가%염작근
滑膜间充质干细胞%细胞因子%趋化%迁移
滑膜間充質榦細胞%細胞因子%趨化%遷移
활막간충질간세포%세포인자%추화%천이
Synovium-derived mesenchymal stem cells%Cytokine%Chemotaxis%Migration
目的 观察血清、转化生长因子(TGF)-β3、血小板衍生生长因子(PDGF)-AB、骨形态发生蛋白(BMP)-2、成纤维细胞生长因子(FGF)对兔滑膜间充质干细胞(SMSCs)迁移的影响.方法 选取含浓度0.1％、0.5％、1.0％、2.0％、5.0％、10.0％、20.0％血清以及含50μg/LTGF-β3、PDG F-AB、BMP-2、FGF的低糖DMEM作用于兔SMSCs.Transwell小室检测其迁移能力.结果 血清、TGF-33、PDGF-AB、BMP-2、FGF均可趋化兔SMSCs迁移,血L清浓度为5.0％时趋化作用较其他浓度组明显,每×100倍视野下迁移细胞数为(169.00±21.74)个,4种因子叶中 PDGF-AB趋化作用较TGF-β3、BMP-2、FGF明显(219.83±11.29比84.50±10.33、146.67±17.91、99.37±12.27).结论 兔SMSCs可在TGF-β3、PDGF-AB、BMP-2、FGF作用下迁移,其中PDGF-AB趋化最明显,可用于促进SMSCs迁移归巢,进行软骨原位再生的修复研究.
目的 觀察血清、轉化生長因子(TGF)-β3、血小闆衍生生長因子(PDGF)-AB、骨形態髮生蛋白(BMP)-2、成纖維細胞生長因子(FGF)對兔滑膜間充質榦細胞(SMSCs)遷移的影響.方法 選取含濃度0.1％、0.5％、1.0％、2.0％、5.0％、10.0％、20.0％血清以及含50μg/LTGF-β3、PDG F-AB、BMP-2、FGF的低糖DMEM作用于兔SMSCs.Transwell小室檢測其遷移能力.結果 血清、TGF-33、PDGF-AB、BMP-2、FGF均可趨化兔SMSCs遷移,血L清濃度為5.0％時趨化作用較其他濃度組明顯,每×100倍視野下遷移細胞數為(169.00±21.74)箇,4種因子葉中 PDGF-AB趨化作用較TGF-β3、BMP-2、FGF明顯(219.83±11.29比84.50±10.33、146.67±17.91、99.37±12.27).結論 兔SMSCs可在TGF-β3、PDGF-AB、BMP-2、FGF作用下遷移,其中PDGF-AB趨化最明顯,可用于促進SMSCs遷移歸巢,進行軟骨原位再生的脩複研究.
목적 관찰혈청、전화생장인자(TGF)-β3、혈소판연생생장인자(PDGF)-AB、골형태발생단백(BMP)-2、성섬유세포생장인자(FGF)대토활막간충질간세포(SMSCs)천이적영향.방법 선취함농도0.1％、0.5％、1.0％、2.0％、5.0％、10.0％、20.0％혈청이급함50μg/LTGF-β3、PDG F-AB、BMP-2、FGF적저당DMEM작용우토SMSCs.Transwell소실검측기천이능력.결과 혈청、TGF-33、PDGF-AB、BMP-2、FGF균가추화토SMSCs천이,혈L청농도위5.0％시추화작용교기타농도조명현,매×100배시야하천이세포수위(169.00±21.74)개,4충인자협중 PDGF-AB추화작용교TGF-β3、BMP-2、FGF명현(219.83±11.29비84.50±10.33、146.67±17.91、99.37±12.27).결론 토SMSCs가재TGF-β3、PDGF-AB、BMP-2、FGF작용하천이,기중PDGF-AB추화최명현,가용우촉진SMSCs천이귀소,진행연골원위재생적수복연구.
Objective To explore the chemotactic migration effects of serum,transforming growth factor (TGF)-β3,platelet derived growtb factor (PDGF)-AB,bone morphogenetic protein (BMP)-2,and fibroblast growth factor (FGF) on rabbit synovium-derived mesenchymal stem cells (SMSCs).Methods The SMSCs were obtained with passage isolation,and then stimulated bv low-glucose DEME containing TGF-β3,PDGF-AB,BMP-2,FGF with 50 μg/L concentration as well as 0.1％,0.5％,1.0％,2.0％,5.0％,10.0％,20.0％ serum.The migration of SMSCs under five different chemotactic factors was observed in Transwell chamber assays.Results TGF-β3,PDGF-AB,BMP-2,FGF and serum consistently enhanced the migration activity of rabbit SMSCs.Compared witb other concentration,5.0％ is the appropriate concentration for serum to induce the migration of rabbit SMSCs.The average numbers of migratory cells (SMSCs) at × 100 magnification was 169.00 ±21.74 in lower chamber under the 5.0％ serum within the Transwell assay.Among the four growth factors,PDGF-AB showed more potent chemotactic effect than otherthree (219.83±11.29 vs.84.50±10.33,146.67±17.91,99.37±12.27).Conclusion The migration of SMSCs from rabbit could be enhanced by TGF-β3,PDGF-AB,BMP-2 and FGF.PDGF-AB is the most potent chemotactic factor among the four growth factors,and may be useful to promote cell homing and cartilage regeneration in situ during cartilage repair.
