CAJ | 학술논문

目的比较雷帕霉素联合顺铂与单用雷帕霉素或单用顺铂对人骨肉瘤细胞增殖和捌亡的影响.方法将人骨肉瘤细胞株随机分为4组:空白组、阳性药1组、阳性药2组和治疗组.阳性药1组应用雷帕霉素20mg/L,阳性药2组应用顺铂10 mg/L,治疗组联合雷帕霉索20mg/L和顺铂10mg/L,空白组加入等量生理盐水.利用噻唑蓝(MTT)比色法俭测雷帕霉素联合顺铂或单用雷帕霉素/顺铂对骨肉瘤细胞的增殖抑制率；流式细胞术检测雷帕霉素联合顺铂或单用雷帕霉素/顺铂对骨肉瘤细胞凋亡率的影响.结果联合应用两种药物对人骨肉瘤的抑制率较单独使用明显增强,且差异有统计学意义(P＜0.05),空白组:0.33±0.02,阳性药1组:0.56±0.05,阳性药2组:0.55±0.03,治疗组:0.95 ±0.01 单独应用雷帕霉素和顺铂,可诱导骨肉瘤细胞凋亡；联合应用时,诱导凋亡作用显著增强,且差异有统计学意义(P＜0.05).结论雷帕霉素与顺铂联合应用,可明显抑制骨肉瘤细胞的增殖并诱导其凋亡,比单独应用效果更佳.
목적 비교뢰파매소연합순박여단용뢰파매소혹단용순박대인골육류세포증식화팔망적영향.방법 장인골육류세포주수궤분위4조:공백조、양성약1조、양성약2조화치료조.양성약1조응용뢰파매소20mg/L,양성약2조응용순박10 mg/L,치료조연합뢰파매색20mg/L화순박10mg/L,공백조가입등량생리염수.이용새서람(MTT)비색법검측뢰파매소연합순박혹단용뢰파매소/순박대골육류세포적증식억제솔；류식세포술검측뢰파매소연합순박혹단용뢰파매소/순박대골육류세포조망솔적영향.결과 연합응용량충약물대인골육류적억제솔교단독사용명현증강,차차이유통계학의의(P＜0.05),공백조:0.33±0.02,양성약1조:0.56±0.05,양성약2조:0.55±0.03,치료조:0.95 ±0.01 단독응용뢰파매소화순박,가유도골육류세포조망；연합응용시,유도조망작용현저증강,차차이유통계학의의(P＜0.05).결론 뢰파매소여순박연합응용,가명현억제골육류세포적증식병유도기조망,비단독응용효과경가.
Objective By the cormparision of the rapamycin combined with cisplatin with the rapamycin or cisplatin alone towards osteosarcoma' s proliferation and apoptosis,to provide new method in treating osteosarcoma in the clinical.Methods Bone sarcoma cell line were randomly divided into 4 groups:The blank group,positive medicine group 1,positive medicine group 2 and the treatment group.to add 20 mg/L rapamycin in the positive medicine group 1,to add 10 mg/L cisplatin In the positive medicine group 2,to add 20 mg/L rapamycin and 10 mg/L cisplatin in the treatment group,and to add the equal amount of normal saline in the blank group.Then detect the Inhibition rate of every group with MMT,andthe apoptosis rate with flow evtometrv.Results to mean ± standard deviation (（x） ±（s）) said.Results The inhibition rate and apoptosis rate of combination towards osteosareoma enhances obviously compared to the separate application,and the difference is statistically significant(P ＜ 0.05).The blank group:0.33 ± 0.02,positive medicine group 1:0.56 ± 0.05,positive medicine group 2:0.55 ± 0.03,The treatment group:0.95 ±0.01.The apoptosis rate of combination towards osteosarcoma enhances obviously compared to the separate application,and the difference is statistically significant(P ＜ 0.05).Conclusion The combination of rapamycin and cisplatin can Inhibit osteosarcoma cell proliferation and induce its apoptosis obviously,better than the separate application.then can trear the osteosarcoma better.