CAJ | 학술논문

目的观察壳聚糖介导细胞因子反应调节因子A(CrmA)对软骨细胞白细胞介素(IL)-1β和肿瘤坏死因子(TNF)-α表达的作用.方法体外培养兔关节软骨细胞,分别加入磷酸盐缓冲液(PBS)、10 mg/L壳聚糖和壳聚糖/pCDNA3.1+CrmA微粒处理6h后,加入10 μg/L IL-1β共培养48 h,采用实时定量聚合酶链反应(Real-time PCR)方法检测软骨细胞中IL-1β和TNF-α mRNA的表达.结果 IL-1 β mRNA在壳聚糖/pCDNA3.1+CrmA处理组(0.55 ±0.08)软骨细胞中的表达明显低于壳聚糖组(0.69±0.06,P＜0.01)和PBS组(0.99 ±0.04,P＜0.01),壳聚糖组软骨细胞IL-1β的表达与PBS组比较差异有统计学意义(p＜0.01).TNF-α mRNA在壳聚糖/pCDNA3.1+ CrmA处理组(0.57±0.07)软骨细胞中的表达与壳聚糖组(0.71±0.05)和PBS组(0.99±0.06)比较均显著下降(P＜0.01),壳聚糖处理组TNF-α的表达与PBS组比较差异有统计学意义(P＜0.01).结论壳聚糖介导CrmA能够明显抑制IL-1β诱导的软骨细胞IL-1β和TNF-α的表达.
목적 관찰각취당개도세포인자반응조절인자A(CrmA)대연골세포백세포개소(IL)-1β화종류배사인자(TNF)-α표체적작용.방법 체외배양토관절연골세포,분별가입린산염완충액(PBS)、10 mg/L각취당화각취당/pCDNA3.1+CrmA미립처리6h후,가입10 μg/L IL-1β공배양48 h,채용실시정량취합매련반응(Real-time PCR)방법검측연골세포중IL-1β화TNF-α mRNA적표체.결과 IL-1 β mRNA재각취당/pCDNA3.1+CrmA처리조(0.55 ±0.08)연골세포중적표체명현저우각취당조(0.69±0.06,P＜0.01)화PBS조(0.99 ±0.04,P＜0.01),각취당조연골세포IL-1β적표체여PBS조비교차이유통계학의의(p＜0.01).TNF-α mRNA재각취당/pCDNA3.1+ CrmA처리조(0.57±0.07)연골세포중적표체여각취당조(0.71±0.05)화PBS조(0.99±0.06)비교균현저하강(P＜0.01),각취당처리조TNF-α적표체여PBS조비교차이유통계학의의(P＜0.01).결론 각취당개도CrmA능구명현억제IL-1β유도적연골세포IL-1β화TNF-α적표체.
Objective To investigate the effects of chitosan/pCDNA3.1 + cytokine rcsponse modifier A (CrmA) nanoparticles on interkin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) expression of chondrocytes.Methods Rabbit chondrocytes were isolated and cultured,and treated with PBS,10 mg/L chitosan (CS) or chitosan/pCDNA3.1 + CrmA nanoparticles,respectively for 6 h.Then 10 μ g/L IL-1β was added into the culture medium.After 48 h,the mRNA expression of IL-13 and TNF-α in chondrocytes was detected by using real-time polymerase chain reaction.Results In chitosan/pCDNA3.1 + CrmA-treated group (0.55 ±0.08),the mRNA expression of IL-1β in chondrocytes was significantly suppressed as compared with corresponding samples of chitosan-treated group (0.69 ± 0.06,P ＜ 0.01) and PBS-treated group (0.99 ±0.04,P ＜ 0.01).There was significant difference in the I L-1 β expression between chitosantreated group and PBS-treated group (P ＜ 0.01).The TNF-α mRNA expression of chondrocytes in chitosan/pCDNA3.1 + CrmA-treated group (0.57 ± 0.07) was lower than that in chitosan-treated group (0.71 ± 0.05,P ＜ 0.01) and PBS-treated group (0.99 ± 0.06,P ＜ 0.01).Significant difference of TNF-α expression between chitoson treated group and PBS treated group was observed (P ＜ 0.01).Conclusion CrmA mediated by chitosan could significantly suppress the mRNA expression of IL-1β and TNF-α of chondrocytes induced bv interkin-1 β.chitosan/pCDNA3.1 * CrmA nanoparticles maybe a potential candidate for therapy of osteoarthritis.