中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
5期
1048-1050
,共3页
胃癌%细胞周期蛋白依赖性激酶抑制蛋白27%基因突变%启动子甲基化
胃癌%細胞週期蛋白依賴性激酶抑製蛋白27%基因突變%啟動子甲基化
위암%세포주기단백의뢰성격매억제단백27%기인돌변%계동자갑기화
Gastric carcinoma%Cyclin dependent kinase inhibitor protein 27%Mutation%Promoter Methylation
目的 探讨胃癌中细胞周期蛋白依赖性激酶抑制蛋白27基因(P27KiP1)蛋白表达、基因突变及启动子甲基化的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)法、聚合酶链式反应-单链构象多态性分析(PCR-SSCP)技术、甲基化特异性聚合酶链式反应(MSP)方法分别检测97例胃癌中P27KiP1基因蛋白表达、基因突变及启动子甲基化,并选取25例正常胃黏膜组织作对照.结果 正常胃黏膜组织中P27KiP1蛋白高表达率明显高于胃癌组(x2=31.02,P＜0.01).早期胃癌组中P27KiP1蛋白高表达率也高于进展期胃癌组(x2=13.55,P＜0.01).正常胃黏膜组织中未检测到基因突变及启动子甲基化,胃癌组织中检测到P27KiP1基因突变及启动子甲基化,但在早期胃癌及进展期胃癌中两者差异无统计学意义(x2 =0.13、0.32,P＞0.05).46例P27KiP1蛋白高表达病例中仅有5例出现启动子甲基化,而在51例P27KiP1蛋白低表达病例中启动子甲基化高达21例,另有5例出现基因突变(x2=17.89,P ＜0.01).结论 P27KiP1蛋白的表达下调或缺失与胃癌的发生发展有关.P27KiP1基因突变及启动子甲基化在胃癌的早期阶段就可能已经发生并保存下来.P27KiP1基因启动子甲基化及基因突变,特别是启动子甲基化可以引起P27KiP1蛋白表达下调或缺失.
目的 探討胃癌中細胞週期蛋白依賴性激酶抑製蛋白27基因(P27KiP1)蛋白錶達、基因突變及啟動子甲基化的關繫.方法 採用免疫組織化學鏈黴菌抗生物素蛋白-過氧化物酶(SP)法、聚閤酶鏈式反應-單鏈構象多態性分析(PCR-SSCP)技術、甲基化特異性聚閤酶鏈式反應(MSP)方法分彆檢測97例胃癌中P27KiP1基因蛋白錶達、基因突變及啟動子甲基化,併選取25例正常胃黏膜組織作對照.結果 正常胃黏膜組織中P27KiP1蛋白高錶達率明顯高于胃癌組(x2=31.02,P＜0.01).早期胃癌組中P27KiP1蛋白高錶達率也高于進展期胃癌組(x2=13.55,P＜0.01).正常胃黏膜組織中未檢測到基因突變及啟動子甲基化,胃癌組織中檢測到P27KiP1基因突變及啟動子甲基化,但在早期胃癌及進展期胃癌中兩者差異無統計學意義(x2 =0.13、0.32,P＞0.05).46例P27KiP1蛋白高錶達病例中僅有5例齣現啟動子甲基化,而在51例P27KiP1蛋白低錶達病例中啟動子甲基化高達21例,另有5例齣現基因突變(x2=17.89,P ＜0.01).結論 P27KiP1蛋白的錶達下調或缺失與胃癌的髮生髮展有關.P27KiP1基因突變及啟動子甲基化在胃癌的早期階段就可能已經髮生併保存下來.P27KiP1基因啟動子甲基化及基因突變,特彆是啟動子甲基化可以引起P27KiP1蛋白錶達下調或缺失.
목적 탐토위암중세포주기단백의뢰성격매억제단백27기인(P27KiP1)단백표체、기인돌변급계동자갑기화적관계.방법 채용면역조직화학련매균항생물소단백-과양화물매(SP)법、취합매련식반응-단련구상다태성분석(PCR-SSCP)기술、갑기화특이성취합매련식반응(MSP)방법분별검측97례위암중P27KiP1기인단백표체、기인돌변급계동자갑기화,병선취25례정상위점막조직작대조.결과 정상위점막조직중P27KiP1단백고표체솔명현고우위암조(x2=31.02,P＜0.01).조기위암조중P27KiP1단백고표체솔야고우진전기위암조(x2=13.55,P＜0.01).정상위점막조직중미검측도기인돌변급계동자갑기화,위암조직중검측도P27KiP1기인돌변급계동자갑기화,단재조기위암급진전기위암중량자차이무통계학의의(x2 =0.13、0.32,P＞0.05).46례P27KiP1단백고표체병례중부유5례출현계동자갑기화,이재51례P27KiP1단백저표체병례중계동자갑기화고체21례,령유5례출현기인돌변(x2=17.89,P ＜0.01).결론 P27KiP1단백적표체하조혹결실여위암적발생발전유관.P27KiP1기인돌변급계동자갑기화재위암적조기계단취가능이경발생병보존하래.P27KiP1기인계동자갑기화급기인돌변,특별시계동자갑기화가이인기P27KiP1단백표체하조혹결실.
Objective To explore the relationship between the expression,mutation and promoter methylation of cyclin dependent kinase inhibitor protein 27 (P27KiP1) gene in gastric carcinoma.Methods Expression of P27KiP1 was investigated by using SP immunohistochemical staining,mutation was assayed by single-strand conformation polymorphism analysis (PCR-SSCP) and Promoter Methylation was assayed by methylation-specific PCR (MSP) in 97 cases of carcinoma,25 cases normal gastric tissues.Results The expression of P27KiP1 in normal gastric cases was higher than its in gastric carcinoma tissues (x2 =31.02,P ＜ 0.01).The expression of P27KiP1 in early gastric cases was also higher than its in advanced gastric carcinoma tissues (x2 =13.55,P ＜0.01).mutation and promoter methylation chould not be assayed in normal gastric tissues aud they were chould be assayed in carcinoma tissues,but there were no difference between early and advanced carcinoma tissues (x2 =0.13,0.32. P ＞ 0.05).There was a inverse association between expression of P27KiP1 and Promoter Methylation,mution (x2 =17.89,P ＜ 0.01).Conclusion The expression of P27KiP1 was associated with the occurrence and development of gastric carcinoma.P27KiP1 gene mutation and promoter methylation maybe occurred in early stage of this carcinoma and they might lead to the down or loss exprssion of P27KiP1 of gastric carcinoma.
