CAJ | 학술논문

目的观察T-bet在胃癌组织中的表达及对患者的无病生存期、总生存期的影响.方法应用免疫组织化学染色技术,检测152例胃癌患者手术标本中T-bet阳性淋巴细胞的浸润数量,观察患者总生存期和无病生存期.结果 T-bet阳性淋巴细胞的浸润数量与肿瘤大小(=2.35,P＜0.05)、浸润深度(t=2.50,P＜0.05)、UICC分期(t=3.40,P＜0.05)有关,差异有统计学意义.所有患者中位无病生存期为28个月,中位总生存期为37个月.Kaplan-Meier生存分析结果表明,T-bet高表达组无病生存期(x2=3.91,P＜0.05)及总生存期(x2=4.00,P＜0.05)均优于低表达组,差异有统计学意义.COX多因素分析显示,调整年龄、性别、肿瘤部位、浸润深度、肿瘤大小和UICC分期后,T-bet高表达组胃癌患者的复发[风险比(HR)=0.56,95％可信区间(95％ CI)=0.32 ～0.98,P＜0.05]及死亡风险降低(HR=0.55,95％ CI=0.30～0.99,P＜0.05),差异有统计学意义(P＜0.05).结论 T-bet阳性淋巴细胞的浸润数量与胃癌患者的生存时间呈正相关,证实了T-bet为1个正性调节因子,对肿瘤微环境的免疫调节有重要意义.
목적 관찰T-bet재위암조직중적표체급대환자적무병생존기、총생존기적영향.방법 응용면역조직화학염색기술,검측152례위암환자수술표본중T-bet양성림파세포적침윤수량,관찰환자총생존기화무병생존기.결과 T-bet양성림파세포적침윤수량여종류대소(=2.35,P＜0.05)、침윤심도(t=2.50,P＜0.05)、UICC분기(t=3.40,P＜0.05)유관,차이유통계학의의.소유환자중위무병생존기위28개월,중위총생존기위37개월.Kaplan-Meier생존분석결과표명,T-bet고표체조무병생존기(x2=3.91,P＜0.05)급총생존기(x2=4.00,P＜0.05)균우우저표체조,차이유통계학의의.COX다인소분석현시,조정년령、성별、종류부위、침윤심도、종류대소화UICC분기후,T-bet고표체조위암환자적복발[풍험비(HR)=0.56,95％가신구간(95％ CI)=0.32 ～0.98,P＜0.05]급사망풍험강저(HR=0.55,95％ CI=0.30～0.99,P＜0.05),차이유통계학의의(P＜0.05).결론 T-bet양성림파세포적침윤수량여위암환자적생존시간정정상관,증실료T-bet위1개정성조절인자,대종류미배경적면역조절유중요의의.
Objective To anabze the expression of T-bet in gastric carcinoma tissues and the impact on the survival time of patients.Methods The number of T-bet positive lymphocyte in the surgical specimens of gastric cancer tissues was detected by using immunohistochemistry in 152 patients.The time of disease-free survival and overall survival were observed.Data was analyzed by using SPSS 13.0.Results The T-bet expression level was significantly correlated with tumor size (t =2.35,P ＜ 0.05),invasion depth (t =2.50,P ＜ 0.05) and UICC stage (t =3.40,P ＜ 0.05).The median time of disease-free survival and overall survival were 28 months and 37 months.Kaplan-Meier survival analysis demonstrated that the disease free survival as well as the overall survival for the group with the higher density of T-bet positive lymphocyte was significantly better than the group with lower T-bet positive lymphocyte (X2 =3.91,P ＜0.05 and x2 =4.00,P ＜ 0.05,respectively).COX multivariate analysis showed that,after regulating age,sex,tumor location,depth of invasion,tumor size and UICC stage,the high infiltration group of T-bet positive lymphocyte had the trend of recurrence [HR =0.56,95％ confidence interval (95％ CI) =0.32-0.98,P ＜ 0.05] and death (HR =0.55,95％ CI =0.30-0.99,P ＜ 0.05) risk reduction in gastric cancer patients,the difference was statistical significance.Conclusion The number of T-bet positive lymphocyte in gastric carcinoma was positively correlated with patient' s survival,which supports the notion that the T-bet was a positive regulator,which played an important role in tumor micro-environment.