中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
6期
1161-1163
,共3页
荣禄%姚友生%林明恩%吕夷松%邓毕华
榮祿%姚友生%林明恩%呂夷鬆%鄧畢華
영록%요우생%림명은%려이송%산필화
间质性膀胱炎%肥大细胞%干细胞因子%白细胞介素-6
間質性膀胱炎%肥大細胞%榦細胞因子%白細胞介素-6
간질성방광염%비대세포%간세포인자%백세포개소-6
Interstitial cystitis%Mast cell%Stern cell factor%Interleukin 6
目的 检测间质性膀胱炎/膀胱疼痛综合征(IC/BPS)中干细胞因子(SCF)、白细胞介素-6(IL-6)和肥大细胞特异性类胰蛋白酶(tryptase)在膀胱上皮中的表达及定位,探讨其相关性及其与肥大细胞增殖的关系.方法 采用免疫组织化学法检测SCF、IL-6及tryptase在IC/BPS和正常膀胱上皮组织中的表达及定位;采用实时定量聚合酶链反应(Real-time PCR)检测IC/BPS和正常膀胱上皮组织中上述3种基因mRNA的表达差异;Western blot检测SCF、tryptase蛋白水平的表达差异.结果 在IC患者膀胱上皮组织中SCF、IL-6和tryptase在mRNA及蛋白水平的表达均显著高于对照组(P<0.05);在IC患者膀胱上皮组织中肥大细胞明显增多,并且表现出与膀胱黏膜层的密切关系,此外在mRNA水平SCF表达量与tryptase表达量呈正相关(r=0.844,P<0.01).结论 IC患者膀胱上皮组织中肥大细胞的增多和激活,可能是由于膀胱黏膜层共表达SCF和IL-6升高过度刺激的结果.
目的 檢測間質性膀胱炎/膀胱疼痛綜閤徵(IC/BPS)中榦細胞因子(SCF)、白細胞介素-6(IL-6)和肥大細胞特異性類胰蛋白酶(tryptase)在膀胱上皮中的錶達及定位,探討其相關性及其與肥大細胞增殖的關繫.方法 採用免疫組織化學法檢測SCF、IL-6及tryptase在IC/BPS和正常膀胱上皮組織中的錶達及定位;採用實時定量聚閤酶鏈反應(Real-time PCR)檢測IC/BPS和正常膀胱上皮組織中上述3種基因mRNA的錶達差異;Western blot檢測SCF、tryptase蛋白水平的錶達差異.結果 在IC患者膀胱上皮組織中SCF、IL-6和tryptase在mRNA及蛋白水平的錶達均顯著高于對照組(P<0.05);在IC患者膀胱上皮組織中肥大細胞明顯增多,併且錶現齣與膀胱黏膜層的密切關繫,此外在mRNA水平SCF錶達量與tryptase錶達量呈正相關(r=0.844,P<0.01).結論 IC患者膀胱上皮組織中肥大細胞的增多和激活,可能是由于膀胱黏膜層共錶達SCF和IL-6升高過度刺激的結果.
목적 검측간질성방광염/방광동통종합정(IC/BPS)중간세포인자(SCF)、백세포개소-6(IL-6)화비대세포특이성류이단백매(tryptase)재방광상피중적표체급정위,탐토기상관성급기여비대세포증식적관계.방법 채용면역조직화학법검측SCF、IL-6급tryptase재IC/BPS화정상방광상피조직중적표체급정위;채용실시정량취합매련반응(Real-time PCR)검측IC/BPS화정상방광상피조직중상술3충기인mRNA적표체차이;Western blot검측SCF、tryptase단백수평적표체차이.결과 재IC환자방광상피조직중SCF、IL-6화tryptase재mRNA급단백수평적표체균현저고우대조조(P<0.05);재IC환자방광상피조직중비대세포명현증다,병차표현출여방광점막층적밀절관계,차외재mRNA수평SCF표체량여tryptase표체량정정상관(r=0.844,P<0.01).결론 IC환자방광상피조직중비대세포적증다화격활,가능시유우방광점막층공표체SCF화IL-6승고과도자격적결과.
Objective To investigate the expression of stem cell factor (SCF),interleukin 6 (IL-6) and tryptase in bladder epithelia,and their relationship with mast cells in interstitial cystitis/bladder pain syndrome (IC/BPS).Methods The protein expression levels and localizations of SCF,IL-6 and tryptase in IC/BPS and normal bladder epithelial tissues were determined by using immunohistochemistry.The mRNA and protein expression levels of SCF,IL-6 and tryptase were detected by using real-time quantitative polymerase chain reaction (PCR) and Western blotting in IC/BPS and normal bladder epithelial tissues respectively.Results The mRNA and protein expression levels of SCF,IL-6 and tryptase in IC/BPS bladder epithelial tissues were higher than those in normal bladder epithelial tissues (both P < 0.05).The number of mast cells in IC/BPS bladder epithelia tissues was significantly greater than in normal bladder epithelial tissues,and mast cells were found in close proximity of bladder epithelial cells.In addition,the mRNA expression levels of SCF and tryptase displayed a strong positive correlation (r =0.844,P < 0.01).Conclusion The responses of mast cells in IC/BPS may be the result of hyperstimulation by local bladder epithelial SCF and IL-6 co-expression.