中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
6期
1177-1180
,共4页
组蛋白乙酰转移酶抑制剂%前列腺癌%脱噬作用%腰果酸
組蛋白乙酰轉移酶抑製劑%前列腺癌%脫噬作用%腰果痠
조단백을선전이매억제제%전렬선암%탈서작용%요과산
Histone acetyltransferase inhibitor%Prostate cancer%Apoptosis%Anacardic acid
目的 观察组蛋白乙酰转移酶抑制剂C646分子联合腰果酸(AA)对雄激素非依赖性前列癌细胞的影响,并探讨其机制.方法 将实验分为4组即对照组、C646组、AA组及联合用药组,通过细胞增殖与毒性检测试剂盒MTS、Transwell实验、流式细胞仪、倒置显微镜等检测药物对细胞的影响.结果 C646半数抑制浓度约为20 μmol/L,AA的安全浓度约为25 μmol/L,C646组、AA组及联合用药组药物分别作用于DU-145细胞后,MTS检测发现细胞存活率分别为0.529％、0.763％、0.239％,联合用药组毒性作用明显增强(P＜0.05)；流式细胞仪检测细胞凋亡及Western blot分析凋亡标志物结果均显示联合用药组凋亡诱导能力较各单独用药组大大增强(P＜0.05)；Transwell实验发现迁移细胞占对照组百分率分别为77.82％、88.35％、65.19％,联合用药组作用最为明显(P＜0.05).结论 两药单用时均能有效抑制前列腺癌细胞株DU145的增殖,并促进其凋亡,两药联用时效果最明显,此外,两种药物对细胞迁移也有一定影响.
目的 觀察組蛋白乙酰轉移酶抑製劑C646分子聯閤腰果痠(AA)對雄激素非依賴性前列癌細胞的影響,併探討其機製.方法 將實驗分為4組即對照組、C646組、AA組及聯閤用藥組,通過細胞增殖與毒性檢測試劑盒MTS、Transwell實驗、流式細胞儀、倒置顯微鏡等檢測藥物對細胞的影響.結果 C646半數抑製濃度約為20 μmol/L,AA的安全濃度約為25 μmol/L,C646組、AA組及聯閤用藥組藥物分彆作用于DU-145細胞後,MTS檢測髮現細胞存活率分彆為0.529％、0.763％、0.239％,聯閤用藥組毒性作用明顯增彊(P＜0.05)；流式細胞儀檢測細胞凋亡及Western blot分析凋亡標誌物結果均顯示聯閤用藥組凋亡誘導能力較各單獨用藥組大大增彊(P＜0.05)；Transwell實驗髮現遷移細胞佔對照組百分率分彆為77.82％、88.35％、65.19％,聯閤用藥組作用最為明顯(P＜0.05).結論 兩藥單用時均能有效抑製前列腺癌細胞株DU145的增殖,併促進其凋亡,兩藥聯用時效果最明顯,此外,兩種藥物對細胞遷移也有一定影響.
목적 관찰조단백을선전이매억제제C646분자연합요과산(AA)대웅격소비의뢰성전렬암세포적영향,병탐토기궤제.방법 장실험분위4조즉대조조、C646조、AA조급연합용약조,통과세포증식여독성검측시제합MTS、Transwell실험、류식세포의、도치현미경등검측약물대세포적영향.결과 C646반수억제농도약위20 μmol/L,AA적안전농도약위25 μmol/L,C646조、AA조급연합용약조약물분별작용우DU-145세포후,MTS검측발현세포존활솔분별위0.529％、0.763％、0.239％,연합용약조독성작용명현증강(P＜0.05)；류식세포의검측세포조망급Western blot분석조망표지물결과균현시연합용약조조망유도능력교각단독용약조대대증강(P＜0.05)；Transwell실험발현천이세포점대조조백분솔분별위77.82％、88.35％、65.19％,연합용약조작용최위명현(P＜0.05).결론 량약단용시균능유효억제전렬선암세포주DU145적증식,병촉진기조망,량약련용시효과최명현,차외,량충약물대세포천이야유일정영향.
Objective To study the effects of C646 combined with anacardic acid (AA) on the androgen-independent prostate cancer (AIPC) cell line and the mechanisms.Methods Four groups were designed in experiment:control,C646,AA,and combination (C646 ± AA).The inhibitory effect of proliferation,migration,and the ability to induce apoptosis,and changes of cell cycles were detected by using MTS,Transwell assay,flow cytometry,inverted microscope,etc.Results The half inhibitory concentration of C646 was about 20 μmol/L,and the safe concentration of AA was 25 μmol/L.MTS showed that the survival rate in C646,AA and combination groups was 0.529％,0.763％,and 0.239％ respectively,and the cytotoxic effects in combination group were obviously enhanced (P ＜ 0.05).The flow cytometry and Western blotting revealed that the apoptosis rate was significantly increased in combination group (P ＜0.05).Transwell assay demonstrated that the proportion of migrating cells in C646,AA and combination groups accounted for 77.82％,88.35％ and 65.19％ of control group,respectively,most significant in combination group (P ＜ 0.05).Conclusion C646 and AA used alone could effectively inhibit the proliferation and promote apoptosis of DU-145 cells in vitro,and combined use of them exerted the synergic effects.In addition,they could inhibit cell migration to some extent.
