中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
6期
1232-1234
,共3页
白细胞介素-6%细胞增殖%生长周期
白細胞介素-6%細胞增殖%生長週期
백세포개소-6%세포증식%생장주기
Interleukin-6%Cell proliferation%Cell circle
目的 观察肝癌细胞株HepG2自身分泌的白细胞介素-6(IL-6)对其增殖能力和细胞周期的影响.方法 RNA干扰技术沉默IL-6基因,同时设空白对照组(不加转染试剂)、正常对照组(转染空脂质体)、无义对照组[转染无义对照小干扰RNA(siRNA)],实验对照组(转染沉默IL-6的siRNA).噻唑蓝(MTT)比色法检测细胞的增殖能力,用流式细胞技术检测细胞的生长周期.结果 逆转录-聚合酶链反应(RT-PCR)检测各组IL-6 mRNA的表达量分别为104.00±10.71、309.00±15.19、162.50±9.85、26.75±7.54; MTT法检测各组细胞增殖率分别为100.00％、160.72％、130.39％、55.54％；流式细胞技术检测空白对照组,正常对照组,无义对照组,实验对照组不同细胞周期时细胞比例分别为:G0/G1期分别为(68.09±0.98)％、(63.28 ±0.92)％、(67.01±0.93)％、(73.43±1.16)％；S期分别为(23.04±0.99)％、(32.32±0.83)％、(25.11±1.24)％、(20.34±0.20)％；S±G2/M期分别为(31.9l±0.98)％、(36.72±0.92)％、(32.99±0.93)％、(26.57±1.16)％.结论 mRNA的RNA干扰技术能有效的抑制IL-6 mRNA在肝癌细胞株HepG2中的表达,IL-6被沉默能抑制肝癌细胞株HepG2的增殖,使其生长周期延长；肝癌细胞株HepG2 IL-6表达增高能促进其增殖,使其生长周期缩短.
目的 觀察肝癌細胞株HepG2自身分泌的白細胞介素-6(IL-6)對其增殖能力和細胞週期的影響.方法 RNA榦擾技術沉默IL-6基因,同時設空白對照組(不加轉染試劑)、正常對照組(轉染空脂質體)、無義對照組[轉染無義對照小榦擾RNA(siRNA)],實驗對照組(轉染沉默IL-6的siRNA).噻唑藍(MTT)比色法檢測細胞的增殖能力,用流式細胞技術檢測細胞的生長週期.結果 逆轉錄-聚閤酶鏈反應(RT-PCR)檢測各組IL-6 mRNA的錶達量分彆為104.00±10.71、309.00±15.19、162.50±9.85、26.75±7.54; MTT法檢測各組細胞增殖率分彆為100.00％、160.72％、130.39％、55.54％；流式細胞技術檢測空白對照組,正常對照組,無義對照組,實驗對照組不同細胞週期時細胞比例分彆為:G0/G1期分彆為(68.09±0.98)％、(63.28 ±0.92)％、(67.01±0.93)％、(73.43±1.16)％；S期分彆為(23.04±0.99)％、(32.32±0.83)％、(25.11±1.24)％、(20.34±0.20)％；S±G2/M期分彆為(31.9l±0.98)％、(36.72±0.92)％、(32.99±0.93)％、(26.57±1.16)％.結論 mRNA的RNA榦擾技術能有效的抑製IL-6 mRNA在肝癌細胞株HepG2中的錶達,IL-6被沉默能抑製肝癌細胞株HepG2的增殖,使其生長週期延長；肝癌細胞株HepG2 IL-6錶達增高能促進其增殖,使其生長週期縮短.
목적 관찰간암세포주HepG2자신분비적백세포개소-6(IL-6)대기증식능력화세포주기적영향.방법 RNA간우기술침묵IL-6기인,동시설공백대조조(불가전염시제)、정상대조조(전염공지질체)、무의대조조[전염무의대조소간우RNA(siRNA)],실험대조조(전염침묵IL-6적siRNA).새서람(MTT)비색법검측세포적증식능력,용류식세포기술검측세포적생장주기.결과 역전록-취합매련반응(RT-PCR)검측각조IL-6 mRNA적표체량분별위104.00±10.71、309.00±15.19、162.50±9.85、26.75±7.54; MTT법검측각조세포증식솔분별위100.00％、160.72％、130.39％、55.54％；류식세포기술검측공백대조조,정상대조조,무의대조조,실험대조조불동세포주기시세포비례분별위:G0/G1기분별위(68.09±0.98)％、(63.28 ±0.92)％、(67.01±0.93)％、(73.43±1.16)％；S기분별위(23.04±0.99)％、(32.32±0.83)％、(25.11±1.24)％、(20.34±0.20)％；S±G2/M기분별위(31.9l±0.98)％、(36.72±0.92)％、(32.99±0.93)％、(26.57±1.16)％.결론 mRNA적RNA간우기술능유효적억제IL-6 mRNA재간암세포주HepG2중적표체,IL-6피침묵능억제간암세포주HepG2적증식,사기생장주기연장；간암세포주HepG2 IL-6표체증고능촉진기증식,사기생장주기축단.
Objective To study the effect of self-secreted interleukin-6 (IL-6) of proliferation and cell cycle of liver cancer cell line HepG2.Methods The interleukin-6 was made to be silence by using RNA interference technology,and then the control groups were established accordindy by using small interfering RNA (siRNA) with insignificant order,liposome only and no transfection and transfected IL-6-RNA.Detect the proliferation and cell cycle by using methyl thiazol tetrazolium (MTT) and flow cytometry technology.Results The expression of IL-6 mRNA were 104.00 ± 10.71,309.00 ± 15.19,162.50 ±9.85,26.75 ±7.54; The cell proliferation rate were 100.00％,160.72％,130.39％,55.54％ by using MTT; the cell proportion in different cell circles was detected by using Flow cytometry technology in different teams as follows:G0/G1 phase account for (68.09 ±0.98)％,(63.28 ±0.92)％,(67.01 ±0.93)％,(73.43±1.16)％; S phase account for (23.04 ±0.99)％,(32.32 ±0.83)％,(25.11 ±1.24)％,(20.34 ±0.20)％; S ±G2/M phase account for (31.91 ±0.98)％,(36.72 ±0.92)％,(32.99 ± 0.93) ％,(26.57 ± 1.16) ％.Conclusion It can supress the expression of IL-6 mRNA in liver cancer cell HepG2 by using RNAi interference technique; it can supress the proliferation and extend the cell circle of liver cancer cell HepG2 when IL-6was silence; its proliferation was promoted and cell circle was shorten when it secreted higher interleukin-6 in liver cancer cell line HepG2.
