中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
6期
1242-1244
,共3页
张伟国%安伟德%苗鑫%邓中慧
張偉國%安偉德%苗鑫%鄧中慧
장위국%안위덕%묘흠%산중혜
胃癌%腹膜转移%CCL22%CC类趋化因子受体-4
胃癌%腹膜轉移%CCL22%CC類趨化因子受體-4
위암%복막전이%CCL22%CC류추화인자수체-4
Gastric carcinoma%Peritoneal metastasis%CCL22%CC chemokine receptor-4
目的 探讨趋化因子CCL22及其受体CC类趋化因子受体-4(CCR4)在胃癌腹膜乳斑区转移的作用.方法 经Dil标记的胃癌细胞株MFC,小鼠腹腔内注射Dil-MFC细胞悬液0.2ml(CCR4表达阳性细胞数为5×106个),注射48 h后,免疫组织化学方法检测大网膜乳斑区和非乳斑区的CCL22、CCR4表达.615小鼠腹腔注射0.2 rnl胃癌细胞株MFC悬液(胃癌细胞数为5×106个),分别于注射后第6、8、10天,采用酶联免疫吸附试验(ELISA)法检测其腹水中CCL22的浓度.结果 苏木素-伊红(HE)染色、免疫组织化学检测可见胃癌腹膜转移早期乳斑区域CCL22、CCR4表达均为阳性,而非乳斑区域没有趋化因子CCL22,也没有胃癌细胞转移.小鼠腹水中CCL22浓度在腹腔注射MFC悬液6、8、10d分别为(345.29±1.98)、(378.29±3.87)、(371.57±2.44) ng/L,随着腹水的增加而升高,与空白组比较差异有统计学意义(P＜0.01).结论 趋化因子CCL22及其受体CCR4在胃癌腹膜乳斑转移中起作用.
目的 探討趨化因子CCL22及其受體CC類趨化因子受體-4(CCR4)在胃癌腹膜乳斑區轉移的作用.方法 經Dil標記的胃癌細胞株MFC,小鼠腹腔內註射Dil-MFC細胞懸液0.2ml(CCR4錶達暘性細胞數為5×106箇),註射48 h後,免疫組織化學方法檢測大網膜乳斑區和非乳斑區的CCL22、CCR4錶達.615小鼠腹腔註射0.2 rnl胃癌細胞株MFC懸液(胃癌細胞數為5×106箇),分彆于註射後第6、8、10天,採用酶聯免疫吸附試驗(ELISA)法檢測其腹水中CCL22的濃度.結果 囌木素-伊紅(HE)染色、免疫組織化學檢測可見胃癌腹膜轉移早期乳斑區域CCL22、CCR4錶達均為暘性,而非乳斑區域沒有趨化因子CCL22,也沒有胃癌細胞轉移.小鼠腹水中CCL22濃度在腹腔註射MFC懸液6、8、10d分彆為(345.29±1.98)、(378.29±3.87)、(371.57±2.44) ng/L,隨著腹水的增加而升高,與空白組比較差異有統計學意義(P＜0.01).結論 趨化因子CCL22及其受體CCR4在胃癌腹膜乳斑轉移中起作用.
목적 탐토추화인자CCL22급기수체CC류추화인자수체-4(CCR4)재위암복막유반구전이적작용.방법 경Dil표기적위암세포주MFC,소서복강내주사Dil-MFC세포현액0.2ml(CCR4표체양성세포수위5×106개),주사48 h후,면역조직화학방법검측대망막유반구화비유반구적CCL22、CCR4표체.615소서복강주사0.2 rnl위암세포주MFC현액(위암세포수위5×106개),분별우주사후제6、8、10천,채용매련면역흡부시험(ELISA)법검측기복수중CCL22적농도.결과 소목소-이홍(HE)염색、면역조직화학검측가견위암복막전이조기유반구역CCL22、CCR4표체균위양성,이비유반구역몰유추화인자CCL22,야몰유위암세포전이.소서복수중CCL22농도재복강주사MFC현액6、8、10d분별위(345.29±1.98)、(378.29±3.87)、(371.57±2.44) ng/L,수착복수적증가이승고,여공백조비교차이유통계학의의(P＜0.01).결론 추화인자CCL22급기수체CCR4재위암복막유반전이중기작용.
Objective To investigate the function of CCL22 and CC chemokine receptor-4 (CCR4) in the milky spots peritoneal metastasis of gastric cancer.Methods Dil-MFC cells (CCR4 expression positive,5 × 106 cells) were injected into mice.The expression of CCR4 and CCL22 in the milky spots region and non-milky sports region was compared by using immunohistochemistry 48 h after injection.The concentrations of CCL22 in ascitic fluid were determined by using enzyme linked immunosorbent assay (ELISA) at 6th,8th and 10th day after 0.2 mL of MFC cells (5 × 106 cells) was injected into the 615 mice.Results Hematoxylin and eosin (HE) staining and immunohistochemistry indicated that in the early stages of peritoneal metastasis,the CCL22 and CCR4 expression was positive on milky spots,but on the non-milky spots region,there was no CCL22 expression and no metastasis of gastric cancer cells.The concentration of CCL22 in bloody ascites of mice was (345.29 ± 1.98),(378.29 ± 3.87) and (371.57 ± 2.44) ng/L respectively at 6th,8th and 10th day after intraperitoneal injection (P ＜ 0.01).Conclusion CCL22 and CCR4 play an important role in the early peritoneal metastasis.
