中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
6期
1245-1247
,共3页
胃癌%微小RNA%微小RNA-98%细胞增殖
胃癌%微小RNA%微小RNA-98%細胞增殖
위암%미소RNA%미소RNA-98%세포증식
Gastric cancer%MicroRNAs%MicroRNA-98%Cell proliferation
目的 观察微小RNA(miR)-98在胃癌组织和细胞中的表达及其在胃癌发生发展中的调控作用.方法 采用探针法实时定量逆转录聚合酶链反应(RT-qPCR)法,检测32例胃癌中心组织和其匹配的癌旁正常组织及胃癌细胞株中miR-98的表达水平,并与临床病理资料进行相关分析；转染miR-98模拟物(mimic)提高miR-98表达水平,观察其对细胞增殖和周期的影响.结果 对32例胃癌组织标本分析,癌中心组织miR-98的表达水平为癌旁正常组织的24.52％,差异有统计学意义(P＜0.01)；胃癌上皮细胞株中miR-98表达水平均显著降低,差异有统计学意义(P＜0.01)；miR-98表达水平与胃癌临床分期和淋巴结转移相关(P＜0.05)；转染miR-98 mimic组细胞增殖率下降至阴性组的40.79％,G0/G1期细胞比例明显增多,S期细胞比例明显减少,差异均有统计学意义(P＜0.05).结论 胃癌组织和细胞中miR-98表达水平下降,miR-98可通过细胞G1期阻滞,抑制细胞增殖,在胃癌发生发展过程中发挥抑癌基因作用.
目的 觀察微小RNA(miR)-98在胃癌組織和細胞中的錶達及其在胃癌髮生髮展中的調控作用.方法 採用探針法實時定量逆轉錄聚閤酶鏈反應(RT-qPCR)法,檢測32例胃癌中心組織和其匹配的癌徬正常組織及胃癌細胞株中miR-98的錶達水平,併與臨床病理資料進行相關分析；轉染miR-98模擬物(mimic)提高miR-98錶達水平,觀察其對細胞增殖和週期的影響.結果 對32例胃癌組織標本分析,癌中心組織miR-98的錶達水平為癌徬正常組織的24.52％,差異有統計學意義(P＜0.01)；胃癌上皮細胞株中miR-98錶達水平均顯著降低,差異有統計學意義(P＜0.01)；miR-98錶達水平與胃癌臨床分期和淋巴結轉移相關(P＜0.05)；轉染miR-98 mimic組細胞增殖率下降至陰性組的40.79％,G0/G1期細胞比例明顯增多,S期細胞比例明顯減少,差異均有統計學意義(P＜0.05).結論 胃癌組織和細胞中miR-98錶達水平下降,miR-98可通過細胞G1期阻滯,抑製細胞增殖,在胃癌髮生髮展過程中髮揮抑癌基因作用.
목적 관찰미소RNA(miR)-98재위암조직화세포중적표체급기재위암발생발전중적조공작용.방법 채용탐침법실시정량역전록취합매련반응(RT-qPCR)법,검측32례위암중심조직화기필배적암방정상조직급위암세포주중miR-98적표체수평,병여림상병리자료진행상관분석；전염miR-98모의물(mimic)제고miR-98표체수평,관찰기대세포증식화주기적영향.결과 대32례위암조직표본분석,암중심조직miR-98적표체수평위암방정상조직적24.52％,차이유통계학의의(P＜0.01)；위암상피세포주중miR-98표체수평균현저강저,차이유통계학의의(P＜0.01)；miR-98표체수평여위암림상분기화림파결전이상관(P＜0.05)；전염miR-98 mimic조세포증식솔하강지음성조적40.79％,G0/G1기세포비례명현증다,S기세포비례명현감소,차이균유통계학의의(P＜0.05).결론 위암조직화세포중miR-98표체수평하강,miR-98가통과세포G1기조체,억제세포증식,재위암발생발전과정중발휘억암기인작용.
Objective To explore the role of microRNA (miR)-98 in gastric tumorigenesis.Methods Total RNA from tissue samples and cell lines was obtained with the Trizol isolation reagent.The real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) was used for detecting the expression of miR-98 in 32 pairs of gastric cancer tissues and matched normal gastric tissues and gastric cancer cell lines.The cell proliferation assay was done with a cell counting kit (CCK-8).DNA content analysis was done by flow cytometry with a FACS Calibur system.Results The expression of miR-98 was significantly lower in gastric cancer tissues than in normal tissues with a median change of 0.24-fold (P ＜ 0.01).The expression of miR-98 was significantly down-regulated in BGC-823 (0.79-fold),AGS (0.74-fold),HGC-27 (0.70-fold),MKN-28 (0.64-fold),and MKN-45 cells (0.28-fold) as compared with GES-1 cells.There was a significant correlation between miR-98 expression and clinical stage (P ＜ 0.05),and lymph node metastasis (P ＜ 0.05).The over-expression of miR-98 resulted in 40.79％ decrease in the rate of cell proliferation (P ＜0.01).DNA content analysis by flow cytometry revealed miR-98 restoration induced an accumulation in the percentage of cells at the G1 phase and a reduction of cells in S phase (P ＜0.05).Conclusion This study revealed that miR-98 is low-expressed in gastric cancer and acts as an anti-oncogene in regulating gastric cell growth.
