CAJ | 학술논문

目的从手术切除的人胃肠道间质瘤组织中建立间质瘤细胞株,并探讨其生物学特性.方法将胃肠间质瘤组织切成小块,置于含20％胎牛血清(FBS)的RPMI 1640培养基中进行培养,待细胞达80％～ 90％融合后消化传代培养,并进行形态学、免疫荧光、免疫组织化学、Westernblot及流式细胞术等生物学特性研究.结果成功建立胃肠道间质瘤(GIST)细胞株,该细胞株可在体外培养生存1个月,传代7～8代.经流式细胞仪的免疫荧光分析显示该细胞:CD117+ CD34+.且所建立的GIST细胞表达c-kit蛋白,STI571抑制所建立的GIST细胞增殖.结论间质瘤细胞株可在体外长期培养,且具有明显的间质瘤细胞特征.
목적 종수술절제적인위장도간질류조직중건립간질류세포주,병탐토기생물학특성.방법 장위장간질류조직절성소괴,치우함20％태우혈청(FBS)적RPMI 1640배양기중진행배양,대세포체80％～ 90％융합후소화전대배양,병진행형태학、면역형광、면역조직화학、Westernblot급류식세포술등생물학특성연구.결과 성공건립위장도간질류(GIST)세포주,해세포주가재체외배양생존1개월,전대7～8대.경류식세포의적면역형광분석현시해세포:CD117+ CD34+.차소건립적GIST세포표체c-kit단백,STI571억제소건립적GIST세포증식.결론 간질류세포주가재체외장기배양,차구유명현적간질류세포특정.
Objective To establish the cell lines from specimens of surgically resected human gastrointestihal stromal tumors (GIST) and to identify their biological characters.Methods The tumor tissues from human GIST were cultured in RPMI 1640 media supplemented with 20％ fetal bovine serum.After growing to 80％-90％ confluence,the cells were detached for subculture and their characteristics,including morphology,immunofluorescence staining,immunohistochemical analysis,Western bloting and flow cytometry were determined.Results GIST cells were successfully established.The cell line was passaged for about 7-8 times in one mouth.Immunofluosescence statining showed CDll7 + CD34 +.Moreover,the GIST cell lines expressed c-kit protein,and STI571 inhibited the proliferation of the GIST cell lines.Conclusion The GIST cell line was successfully established in vitro and had specific biological characters of GIST.