CAJ | 학술논문

目的建立大鼠慢传输型便秘(STC)模型,观察STC组大鼠结肠黏膜上的水通道蛋白1(AQP1)表达变化,探讨其与STC的关系.方法将32只SD大鼠随机分为对照组(n=16)和STC组(n=16),采用复方地芬诺酯灌胃的方法建立大鼠慢传输便秘模型成功后,利用逆转录聚合酶链反应(RT-PCR)方法测定STC组及对照组大鼠升降结肠黏膜的AQP1 mRNA表达.结果 STC组大鼠日均粪便质量[(1.279 ±0.743) g/100 g]比对照组[(1.571 ±0.783) g/100 g]明显减少(P＜0.05)；大鼠消化道传输速度检测STC组首粒黑便排出时间为(461.6 ± 150.8)min,较对照组大鼠[(351.3 ±119.9) min]显著延长(P＜0.05).实验组和对照组升结肠AQP1灰度比平均值分别是0.281 ±0.212和0.502 ±0.239(P＜ 0.05)、降结肠AQP1灰度比平均值分别是0.429±0.309和0.509 ±0.308(P ＞0.05).结论 AQP1在STC组升结肠黏膜表达下调,可能对STC的发生、发展有一定的作用.
목적 건립대서만전수형편비(STC)모형,관찰STC조대서결장점막상적수통도단백1(AQP1)표체변화,탐토기여STC적관계.방법 장32지SD대서수궤분위대조조(n=16)화STC조(n=16),채용복방지분낙지관위적방법건립대서만전수편비모형성공후,이용역전록취합매련반응(RT-PCR)방법측정STC조급대조조대서승강결장점막적AQP1 mRNA표체.결과 STC조대서일균분편질량[(1.279 ±0.743) g/100 g]비대조조[(1.571 ±0.783) g/100 g]명현감소(P＜0.05)；대서소화도전수속도검측STC조수립흑편배출시간위(461.6 ± 150.8)min,교대조조대서[(351.3 ±119.9) min]현저연장(P＜0.05).실험조화대조조승결장AQP1회도비평균치분별시0.281 ±0.212화0.502 ±0.239(P＜ 0.05)、강결장AQP1회도비평균치분별시0.429±0.309화0.509 ±0.308(P ＞0.05).결론 AQP1재STC조승결장점막표체하조,가능대STC적발생、발전유일정적작용.
Objective To investigate the expression of aquaporinl in colonic mucosa of rats with slow transit constipation (STC),and its role in the pathologenesis of STC.Methods Thirty-two SpragneDawley rats were randomly divided into two groups:control group (n =16) and STC group (n =16).STC model was created by giving the rats the solution of compound diphenoxylate.The reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of apuaporin 1 (AQP1) mRNA in colonic mucosa of rats with STC (trial group) and the normal rats (control group).Results The fecal mass of STC rats was (1.279 ± 0.743) g/100 g,significantly less than that in the normal rats [(1.571 ±0.783) g/100 g] (P ＜ 0.05).In STC rats,the time of discharge of the first black fecal was 461.6 ±150.8 min,significantly longer than that in the normal rats [(351.3 ± 119.9) min] (P ＜ 0.05).RT-PCR revealed that the average values of gray scale ratios of AQP1 in proximal colon of trial group and control group were 0.281 ± 0.212 and 0.502 ± 0.239 respectively (P ＜ 0.05).The average values of gray scale ratios of AQP1 in distal colon of trial group and control group were 0.429 ± 0.309 and 0.509 ± 0.308 respectively (P ＞0.05).Conclusion Expression of AQP1 in proximal colon of rats with STC was downregulated,which may play roles in water absorption.