中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
8期
1583-1584
,共2页
钟宏%冯毅%郑贵雄%梁彦%冯旭
鐘宏%馮毅%鄭貴雄%樑彥%馮旭
종굉%풍의%정귀웅%량언%풍욱
非小细胞肺癌%细胞周期调节蛋白A1%乳腺癌易感基因1%甲基化
非小細胞肺癌%細胞週期調節蛋白A1%乳腺癌易感基因1%甲基化
비소세포폐암%세포주기조절단백A1%유선암역감기인1%갑기화
Non-small cell lung cancer%Cyclin A1%Breast vancer dusceptibility gene 1%Methylation
目的 探讨细胞周期调节蛋白A1(细胞周期素A1,CCNA1)及乳腺癌易感基因1(BRCA1)甲基化在非小细胞肺癌(NSCLC)患者中的发生频率.方法 采用甲基化特异性聚合酶链反应(MSP)方法检测50例临床上经病理确诊为非小细胞肺癌患者(实验组)和20例良性肺部疾病患者(对照组)CCNA1及BRCA1甲基化的状态,以建立广西非小细胞肺癌相关基因的甲基化谱式.结果 在50例非小细胞肺癌患者CCNA1及BRCA1甲基化率分别为70.0% (35/50)和84.0%(42/50).对照组中20例良性肺部疾病患者这2个基因均为去甲基化.结论 检测非小细胞肺癌患者CCNA1及BRCA1基因DNA甲基化状态有助于诊断非小细胞肺癌.
目的 探討細胞週期調節蛋白A1(細胞週期素A1,CCNA1)及乳腺癌易感基因1(BRCA1)甲基化在非小細胞肺癌(NSCLC)患者中的髮生頻率.方法 採用甲基化特異性聚閤酶鏈反應(MSP)方法檢測50例臨床上經病理確診為非小細胞肺癌患者(實驗組)和20例良性肺部疾病患者(對照組)CCNA1及BRCA1甲基化的狀態,以建立廣西非小細胞肺癌相關基因的甲基化譜式.結果 在50例非小細胞肺癌患者CCNA1及BRCA1甲基化率分彆為70.0% (35/50)和84.0%(42/50).對照組中20例良性肺部疾病患者這2箇基因均為去甲基化.結論 檢測非小細胞肺癌患者CCNA1及BRCA1基因DNA甲基化狀態有助于診斷非小細胞肺癌.
목적 탐토세포주기조절단백A1(세포주기소A1,CCNA1)급유선암역감기인1(BRCA1)갑기화재비소세포폐암(NSCLC)환자중적발생빈솔.방법 채용갑기화특이성취합매련반응(MSP)방법검측50례림상상경병리학진위비소세포폐암환자(실험조)화20례량성폐부질병환자(대조조)CCNA1급BRCA1갑기화적상태,이건립엄서비소세포폐암상관기인적갑기화보식.결과 재50례비소세포폐암환자CCNA1급BRCA1갑기화솔분별위70.0% (35/50)화84.0%(42/50).대조조중20례량성폐부질병환자저2개기인균위거갑기화.결론 검측비소세포폐암환자CCNA1급BRCA1기인DNA갑기화상태유조우진단비소세포폐암.
Objective To explore the frequency of Cyclin A1 (CCNA1) and breast vancer dusceptibility gene 1 (BRCA1) gene methylation in patients with non-small cell lung cancer (NSCLC).Methods The methylation specific polymerase chain reaction (MSP) was used to measure the gene methylation status of CCNA1 and BRCA1 in 50 patients with clinically pathologically confirmed NSCLC (experimental group) and 20 patients with benign pulmonary diseases (control group) in order to establish the spectral type of Guangxi NSCLC related genes.Results In 50 cases of NSCLC,CCNA1 and BRCA1 methylation rate was 70.0% (35/50) and 84.0% (42/50) respectively.In control group,the DNA of these two genes was demethylated.Conclusion The detection of CCNA1 and BRCA1 gene DNA methylation status is beneficial for the diagnosis of Guangxi NSCLC.