中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
8期
1585-1588
,共4页
吴江华%王耀勇%王桦%叶旭军
吳江華%王耀勇%王樺%葉旭軍
오강화%왕요용%왕화%협욱군
非小细胞肺癌%肿瘤坏死因子%基因多态性
非小細胞肺癌%腫瘤壞死因子%基因多態性
비소세포폐암%종류배사인자%기인다태성
Non small-cell lung cancer%Tumor necrosis factor%Gene polymorphism
目的 探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因多态性及单倍型与非小细胞肺癌(NSCLC)的关系.方法 收集NSCLC患者592例,健康对照者636例,聚合酶链反应(PCR)扩增TRAIL目的基因后,直接测序检测TRAIL基因3’非编码区(G1525A/G1588A/C1595T)3种单核苷酸多态性,并做TRAIL单倍型分析.结果 与对照组比较,TRAIL G1525A突变等位基因(A)和基因型(GA+ AA)的频率在NSCLC组中明显降低(P＜0.01)；NSCLC组TRAIL GI588A和C1595T两位点突变等位基因(A)和(T)的频率亦明显低于对照组(P＜0.01).进一步分层分析显示,Ⅰ期+Ⅱ期NSCLC患者中TRAIL C1595T突变等位基因(T)和(CT+ TT)基因型频率分别为47.89％和62.01％,Ⅲ期+Ⅳ期NSCLC患者中分别为58.80％和74.65％,两组差异均有统计学意义[优势比(OR)值分别=1.553和1.804,95％可信区间(CI):1.234 ～1.955和1.268～2.567,P＜0.01].Ⅲ期+Ⅳ期NSCLC患者中TRAIL G1525A突变等位基因(A)的频率为47.54％,较Ⅰ期+Ⅱ期NSCLC患者明显增加(40.75％,OR=1.318,95％ CI:1.658 ～1.047,P＜0.05).单倍型分析TRAIL基因(G1525A/G1588A/C1595T)3个位点紧密连锁,NSCLC组中AAT单倍型频率显著低于对照组(42.45％比58.23％,95％CI:1.525～2.824,P＜0.01)；GAT单倍型频率在NSCLC组中明显增高(9.98％比0.21％,95％ CI:0.015～0.059,P＜0.01).结论 TRAIL(G1525A/G1588A/C1595T)基因多态性及单倍型与NSCLC的易感性密切相关.
目的 探討腫瘤壞死因子相關凋亡誘導配體(TRAIL)基因多態性及單倍型與非小細胞肺癌(NSCLC)的關繫.方法 收集NSCLC患者592例,健康對照者636例,聚閤酶鏈反應(PCR)擴增TRAIL目的基因後,直接測序檢測TRAIL基因3’非編碼區(G1525A/G1588A/C1595T)3種單覈苷痠多態性,併做TRAIL單倍型分析.結果 與對照組比較,TRAIL G1525A突變等位基因(A)和基因型(GA+ AA)的頻率在NSCLC組中明顯降低(P＜0.01)；NSCLC組TRAIL GI588A和C1595T兩位點突變等位基因(A)和(T)的頻率亦明顯低于對照組(P＜0.01).進一步分層分析顯示,Ⅰ期+Ⅱ期NSCLC患者中TRAIL C1595T突變等位基因(T)和(CT+ TT)基因型頻率分彆為47.89％和62.01％,Ⅲ期+Ⅳ期NSCLC患者中分彆為58.80％和74.65％,兩組差異均有統計學意義[優勢比(OR)值分彆=1.553和1.804,95％可信區間(CI):1.234 ～1.955和1.268～2.567,P＜0.01].Ⅲ期+Ⅳ期NSCLC患者中TRAIL G1525A突變等位基因(A)的頻率為47.54％,較Ⅰ期+Ⅱ期NSCLC患者明顯增加(40.75％,OR=1.318,95％ CI:1.658 ～1.047,P＜0.05).單倍型分析TRAIL基因(G1525A/G1588A/C1595T)3箇位點緊密連鎖,NSCLC組中AAT單倍型頻率顯著低于對照組(42.45％比58.23％,95％CI:1.525～2.824,P＜0.01)；GAT單倍型頻率在NSCLC組中明顯增高(9.98％比0.21％,95％ CI:0.015～0.059,P＜0.01).結論 TRAIL(G1525A/G1588A/C1595T)基因多態性及單倍型與NSCLC的易感性密切相關.
목적 탐토종류배사인자상관조망유도배체(TRAIL)기인다태성급단배형여비소세포폐암(NSCLC)적관계.방법 수집NSCLC환자592례,건강대조자636례,취합매련반응(PCR)확증TRAIL목적기인후,직접측서검측TRAIL기인3’비편마구(G1525A/G1588A/C1595T)3충단핵감산다태성,병주TRAIL단배형분석.결과 여대조조비교,TRAIL G1525A돌변등위기인(A)화기인형(GA+ AA)적빈솔재NSCLC조중명현강저(P＜0.01)；NSCLC조TRAIL GI588A화C1595T량위점돌변등위기인(A)화(T)적빈솔역명현저우대조조(P＜0.01).진일보분층분석현시,Ⅰ기+Ⅱ기NSCLC환자중TRAIL C1595T돌변등위기인(T)화(CT+ TT)기인형빈솔분별위47.89％화62.01％,Ⅲ기+Ⅳ기NSCLC환자중분별위58.80％화74.65％,량조차이균유통계학의의[우세비(OR)치분별=1.553화1.804,95％가신구간(CI):1.234 ～1.955화1.268～2.567,P＜0.01].Ⅲ기+Ⅳ기NSCLC환자중TRAIL G1525A돌변등위기인(A)적빈솔위47.54％,교Ⅰ기+Ⅱ기NSCLC환자명현증가(40.75％,OR=1.318,95％ CI:1.658 ～1.047,P＜0.05).단배형분석TRAIL기인(G1525A/G1588A/C1595T)3개위점긴밀련쇄,NSCLC조중AAT단배형빈솔현저저우대조조(42.45％비58.23％,95％CI:1.525～2.824,P＜0.01)；GAT단배형빈솔재NSCLC조중명현증고(9.98％비0.21％,95％ CI:0.015～0.059,P＜0.01).결론 TRAIL(G1525A/G1588A/C1595T)기인다태성급단배형여NSCLC적역감성밀절상관.
Objective To explore the associations between genetic polymorphisms and haplotypes of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) and patients with non small-cell lung cancer (NSCLC).Methods A total of 592 patients with NSCLC and 636 healthy controls were collected.After PCR amplification,TRAIL (G1525A/G1588A/CI595T) gene polymorphisms were detected by using direct sequencing.Haplotype analysis was also performed on all study subjects.Results Frequencies of variant allele (A) and genotype (GA + AA) in TRAIL G1525A were significantly lower in NSCLC patients than in healthy controls (both P＜0.01).Frequencies of variant allele (A) and (T) in TRAIL G1588A and C1595T were also significantly lower in NSCLC patients than those in the healthy controls (both P ＜ 0.01).In the further stratification analysis,frequencies of variant allele (T) and genotype (CT + TT) in TRAIL C1595T significantly differed between (stage Ⅰ + Ⅱ) and (stage Ⅲ + Ⅳ) NSCLC patients [47.89％ vs.58.80％,OR =2.710,95％ confidence interval (CI):1.598-4.596 ; 62.01％ vs.74.65％,OR =2.935,95 ％ CI:1.188-7.249,respectively,both P ＜ 0.05).Moreover,frequency of variant allele (A) in TRAIL G1525A was significantly higher in (stage Ⅲ+ Ⅳ) NSCLC patients than that in (stage Ⅰ + Ⅱ) NSCLC patients (47.54％ vs.40.75％,OR =1.318,95 ％ CI:1.658-1.047,P ＜ 0.05).In addition,TRAIL (G1525A/G1588A/C1595T) genes were found to be in a complete disequilibrium linkage in all study subjects.In contrast with healthy controls,frequency of AAT haplotype was significantly decreased (42.45％ vs.58.23％,95％ CI:1.525-2.824,P ＜0.01),wherease frequency of GAT haplotype was significantly increased in NSCLC patients (9.98％ vs.0.21％,95％ CI:0.015-0.059,P ＜0.01).Conclusion Genetic polymorphisms and haplotypes of TRAIL (G1525A/G1588A/C1595T) genes may be significantly correlated with the susceptibility to NSCLC in Chinese patients.
