CAJ | 학술논문

目的观察部分液体通气对无心跳供体(NHBD)肺的保护作用.方法 36只清洁级SD大鼠随机分为3组:氧气组(C组)、盐水组(B组)和部分液体通气组(A组).建立NHBD肺模型后行机械通气2h并监测大鼠气道压,2h后测量P-V曲线,切除大鼠左肺下叶,观察供体肺病理特点.行右肺支气管灌洗,测量灌洗液中肺表面活性物质相关蛋白B、C(SP-B、SP-C)的浓度.切除大鼠右肺上叶,并进一步行SP-B、SP-C的实时定量聚合酶链反应(Real-time PCR)检测.结果 A组大鼠动态顺应性和静态顺应性在2h热缺血期间都优于B和C组(P＜0.05)；病理组织评分A组优于B组和C组(P＜0.05)；肺泡灌洗液酶联免疫吸附试验(ELISA)检测和肺组织的Real-time PCR检测说明SP-B和SP-C蛋白和RNA含量差异有统计学意义,其中A组含量明显高于B组和C组(P＜0.05).结论部分液体通气可以对热缺血期间供肺的肺功能和肺组织起到很好的保护作用,并对SP-B和SP-C有较好的保护作用.
목적 관찰부분액체통기대무심도공체(NHBD)폐적보호작용.방법 36지청길급SD대서수궤분위3조:양기조(C조)、염수조(B조)화부분액체통기조(A조).건립NHBD폐모형후행궤계통기2h병감측대서기도압,2h후측량P-V곡선,절제대서좌폐하협,관찰공체폐병리특점.행우폐지기관관세,측량관세액중폐표면활성물질상관단백B、C(SP-B、SP-C)적농도.절제대서우폐상협,병진일보행SP-B、SP-C적실시정량취합매련반응(Real-time PCR)검측.결과 A조대서동태순응성화정태순응성재2h열결혈기간도우우B화C조(P＜0.05)；병리조직평분A조우우B조화C조(P＜0.05)；폐포관세액매련면역흡부시험(ELISA)검측화폐조직적Real-time PCR검측설명SP-B화SP-C단백화RNA함량차이유통계학의의,기중A조함량명현고우B조화C조(P＜0.05).결론 부분액체통기가이대열결혈기간공폐적폐공능화폐조직기도흔호적보호작용,병대SP-B화SP-C유교호적보호작용.
Objective To investigate the protection of non-heart-beating-donor lung with part liquid ventilation.Methods Thirty-six health SD rats were randomly divided into three groups,n =12:oxygen group (group C),salt water group (group B),and part liquid ventilation group (group A).Mechanical ventilation was done for 2 h after establishment of non-heart-beating donor lung model,and airway pressure was measured continuously during 2 h.After 2 h,P-V curve was measured.Resection the under leaves of the rats,observe the different pathological characteristics by electron microscopy of the lung.Right lung was taking irrigation,measurement of bronchial lung lavaged suffactant protein B,C (SP-B,the SP-C) concentrations.Cut the upper lobe toward the right lung and measurement SP-B and SP-C with real-time quantitative polymerase chain reaction (Real-time PCR) testing.Results The dynamic compliance and static compliance of group A are better than group B and C during 2 hours hot ischemia(P ＜0.05) ;Pathology organization score of group A is better than group B and C (P ＜ 0.05) ; SP-B and SP-C enzyme linked immunosorbent assay (ELISA) test and lung tissue of the Real-time PCR testing instructions protein and RNA content are difference,group A is higher than group B and C (P ＜ 0.05).Conclusion Part liquid ventilation protect pulmonary lung function and lung tissue well during heat during ischemia,and have very good protection effect to the SP-B and SP-C.