CAJ | 학술논문

目的观察大黄素在体外对胆管癌QBC939细胞生长的抑制作用及其对磷酸肌醇3激酶(PI3K)/蛋白激酶B(Akt)/雷帕霉素靶蛋白(mTOR)信号转导通路的影响.方法用大黄素作为干预因素,时间效应组以含大黄素的培养液分别培养QBC939细胞不同时间,剂量效应组分别用不同浓度大黄素的培养液与QBC939细胞共培养；检测细胞增殖,逆转录-聚合酶链反应(RT-PCR)检测细胞中B淋巴细胞/白血病-2 (bcl-2) mRNA表达,Western blot检测细胞中bcl-2、Akt、磷酸化Akt (p-Akt)、核因子(NF)-κB、磷酸化NF-κB(p-NF-κB)、mTOR、磷酸化mTOR(p-mTOR)蛋白质的表达.结果 10、20、40、80 μmol/L大黄素对QBC939细胞增殖抑制率分别为17.3％、28.6％、46.5％和66.4％ (P ＜0.05),bcl-2、p-Akt、NF-κB、p-NF-κB、mTOR、p-mTOR表达明显下降,Akt表达无变化.结论大黄素抑制QBC939细胞增殖,可能通过抑制PI3K/Akt/mTOR信号转导途径.
목적 관찰대황소재체외대담관암QBC939세포생장적억제작용급기대린산기순3격매(PI3K)/단백격매B(Akt)/뢰파매소파단백(mTOR)신호전도통로적영향.방법 용대황소작위간예인소,시간효응조이함대황소적배양액분별배양QBC939세포불동시간,제량효응조분별용불동농도대황소적배양액여QBC939세포공배양；검측세포증식,역전록-취합매련반응(RT-PCR)검측세포중B림파세포/백혈병-2 (bcl-2) mRNA표체,Western blot검측세포중bcl-2、Akt、린산화Akt (p-Akt)、핵인자(NF)-κB、린산화NF-κB(p-NF-κB)、mTOR、린산화mTOR(p-mTOR)단백질적표체.결과 10、20、40、80 μmol/L대황소대QBC939세포증식억제솔분별위17.3％、28.6％、46.5％화66.4％ (P ＜0.05),bcl-2、p-Akt、NF-κB、p-NF-κB、mTOR、p-mTOR표체명현하강,Akt표체무변화.결론 대황소억제QBC939세포증식,가능통과억제PI3K/Akt/mTOR신호전도도경.
Objective To investigate the effects of Emodin on proliferation inhibition of cholangiocarcinoma QBC939 cells and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) in vitro.Methods QBC939 cells were cultured with Emodin for different time periods,or with different concentrations of Emodin.The proliferation inhibition of cholangiocarcinoma QBC939 cells was detected by using methyl thiazol tetrazolium (MTT) assay.The expression of B lymphocytes/leukemia-2 (bcl-2) mRNA and protein was detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting.Akt,p-Akt,nuclear factor-κB (NF-κB),phosphorylated NF-κB (p-NF-κB),mTOR,and phosphorylated mTOR (p-mTOR) were examined by using Western blotting.Results Inhibitory rate of QBC939 cells treated with 10,20,40 and 80 μmol/L Emodin for 48 h was 17.3％,28.6％,46.5％,and 66.4％ respectively (P ＜0.05).Emodin could down-regulate the expression levels of bcl-2,p-Akt,NF-κB,p-NF-κB,mTOR and p-mTOR (P ＜ 0.05),but had no signficnat effect on the Akt expression.Conclusion Emodin can inhibit proliferation of QBC939 cells probably by down-regulating PI3K/Akt/mTOR.